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AB172012

Anti-Kisspeptin antibody

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(1 Publication)

Mouse Polyclonal Kisspeptin antibody. Carrier free. Suitable for WB and reacts with Transfected cell lysate samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KISS1.

View Alternative Names

PP5098, KISS1, Metastasis-suppressor KiSS-1, Kisspeptin-1

1 Images
Western blot - Anti-Kisspeptin antibody (AB172012)
  • WB

Supplier Data

Western blot - Anti-Kisspeptin antibody (AB172012)

All lanes:

Western blot - Anti-Kisspeptin antibody (ab172012) at 1 µg/mL

Lane 1:

Kisspeptin 293T transfected lysate at 15 µL

Lane 2:

Non-transfected 293T lysate at 15 µL

Predicted band size: 15 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KISS1.

Q15726

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Kisspeptin also known as metastin is a peptide with significant roles in reproductive physiology. Found within the hypothalamus and several peripheral tissues it comprises a sequence of 54 amino acids and has a molecular mass of around 12.8 kDa. Kisspeptin binds to the G protein-coupled receptor GPR54 facilitating its regulatory functions. Widely expressed throughout the brain particularly in the arcuate nucleus and anteroventral periventricular nucleus it acts on neurons to influence GnRH secretion critically.
Biological function summary

Kisspeptin regulates the hypothalamic-pituitary-gonadal axis by promoting the release of gonadotropin-releasing hormone (GnRH). This stimulation enhances the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary gland key components in reproductive maturation and fertility. Kisspeptin integrates into the neuroendocrine control network interacting closely with neurotransmitters and signaling molecules to maintain reproductive homeostasis.

Pathways

Kisspeptin plays an instrumental role in the reproductive signaling cascade. It serves as a critical upstream regulator in the GnRH signaling pathway essential for the control of puberty onset and reproductive function. The kisspeptin-GPR54 axis interacts with related proteins like neurokinin B and dynorphin which modulate its effect on GnRH neurons. These interactions create an intricate regulatory network essential to reproductive hormone feedback loops.

Altered kisspeptin signaling can contribute to conditions such as hypogonadotropic hypogonadism and polycystic ovary syndrome (PCOS). Mutations or deficiencies in the kisspeptin receptor GPR54 have been linked to hypogonadotropic hypogonadism impacting sexual development and fertility. PCOS involves complex kisspeptin-related pathways alongside insulin signaling dysregulation illustrating its intersection with metabolic pathways and potential treatment targets. Kisspeptin modulation affects significant pathways related to reproductive health making it a target of interest for therapeutic intervention in these conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
See full target information KISS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open life sciences 16:667-681 PubMed34250246

2021

Long noncoding RNA HULC contributes to paclitaxel resistance in ovarian cancer via miR-137/ITGB8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Huang,Min Wei,Li Hong
View all publications
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