Rabbit Polyclonal Kisspeptin antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KISS1.
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Publications
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- Acta biochimica Polonica 70:183-1902023Timosaponin AIII attenuates precocious puberty in mice through downregulating the hypothalamic-pituitary-gonadal axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLili Zhou,Yaoquan Ren,Dongmei Li,Weiwei Zhou,Chengke Li,Qiang Wang,Xiangzheng YangPubMed 36928746
- Frontiers in microbiology 13:9307472022Effects of High-Fat Diet During Childhood on Precocious Puberty and Gut Microbiota in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTingbei Bo,Min Liu,Liqiu Tang,Jinzhen Lv,Jing Wen,Dehua WangPubMed 35910597
- Reproductive biology and endocrinology : RB&E 20:222022TGF-β1 inhibits human trophoblast cell invasion by upregulating kisspeptin expression through ERK1/2 but not SMAD signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanlan Fang,Yang Yan,Yibo Gao,Ze Wu,Zhen Wang,Sizhu Yang,Jung-Chien Cheng,Ying-Pu SunPubMed 35101033
- Cell communication and signaling : CCS 19:1012021EGF stimulates human trophoblast cell invasion by downregulating ID3-mediated KISS1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLanlan Fang,Yibo Gao,Zhen Wang,Yuxi Li,Yang Yan,Ze Wu,Jung-Chien Cheng,Ying-Pu SunPubMed 34620174
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KISS1. The exact immunogen used to generate this antibody is proprietary information. Database link Q15726
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
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Target data
Function
Metastasis suppressor protein in malignant melanomas and in some breast cancers. May regulate events downstream of cell-matrix adhesion, perhaps involving cytoskeletal reorganization. Generates a C-terminally amidated peptide, metastin which functions as the endogenous ligand of the G-protein coupled receptor GPR54. Activation of the receptor inhibits cell proliferation and cell migration, key characteristics of tumor metastasis. Kp-10 is a decapeptide derived from the primary translation product, isolated in conditioned medium of first trimester trophoblast. Kp-10, but not other kisspeptins, increased intracellular Ca(2+) levels in isolated first trimester trophoblasts. Kp-10 is a paracrine/endocrine regulator in fine-tuning trophoblast invasion generated by the trophoblast itself. The receptor is also essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/GPR54 system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood.
Alternative names
Metastasis-suppressor KiSS-1, Kisspeptin-1, PP5098, KISS1
Recommended products
Rabbit Polyclonal Kisspeptin antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KISS1.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KISS1. The exact immunogen used to generate this antibody is proprietary information. Database link Q15726
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Kisspeptin also known as metastin is a peptide with significant roles in reproductive physiology. Found within the hypothalamus and several peripheral tissues it comprises a sequence of 54 amino acids and has a molecular mass of around 12.8 kDa. Kisspeptin binds to the G protein-coupled receptor GPR54 facilitating its regulatory functions. Widely expressed throughout the brain particularly in the arcuate nucleus and anteroventral periventricular nucleus it acts on neurons to influence GnRH secretion critically.
- Biological function summary
Kisspeptin regulates the hypothalamic-pituitary-gonadal axis by promoting the release of gonadotropin-releasing hormone (GnRH). This stimulation enhances the synthesis and release of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) from the pituitary gland key components in reproductive maturation and fertility. Kisspeptin integrates into the neuroendocrine control network interacting closely with neurotransmitters and signaling molecules to maintain reproductive homeostasis.
- Pathways
Kisspeptin plays an instrumental role in the reproductive signaling cascade. It serves as a critical upstream regulator in the GnRH signaling pathway essential for the control of puberty onset and reproductive function. The kisspeptin-GPR54 axis interacts with related proteins like neurokinin B and dynorphin which modulate its effect on GnRH neurons. These interactions create an intricate regulatory network essential to reproductive hormone feedback loops.
- Associated diseases and disorders
Altered kisspeptin signaling can contribute to conditions such as hypogonadotropic hypogonadism and polycystic ovary syndrome (PCOS). Mutations or deficiencies in the kisspeptin receptor GPR54 have been linked to hypogonadotropic hypogonadism impacting sexual development and fertility. PCOS involves complex kisspeptin-related pathways alongside insulin signaling dysregulation illustrating its intersection with metabolic pathways and potential treatment targets. Kisspeptin modulation affects significant pathways related to reproductive health making it a target of interest for therapeutic intervention in these conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-Kisspeptin antibody (ab226786)
15% SDS-PAGE gel.
All lanes: Western blot - Anti-Kisspeptin antibody (ab226786) at 1/1000 dilution
All lanes: Mouse testis tissue extract at 50 µg
Predicted band size: 15 kDa
Western blot - Anti-Kisspeptin antibody (ab226786)
15% SDS-PAGE gel.
All lanes: Western blot - Anti-Kisspeptin antibody (ab226786) at 1/1000 dilution
Lane 1: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract at 30 µg
Lane 2: Raji (human Burkitt's lymphoma cell line) whole cell extract at 30 µg
Lane 3: NCI-H929 whole cell extract at 30 µg
Predicted band size: 15 kDa
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