Rabbit Polyclonal KLB antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications.
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Publications
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- Heliyon 9:e149522023FGF21/FGFR1-β-KL cascade in cardiomyocytes modulates angiogenesis and inflammation under metabolic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNamrita Kaur,Sanskruti Ravindra Gare,Andrea Ruiz-Velasco,Jessica M Miller,Riham R E Abouleisa,Qinghui Ou,Jiahan Shen,Handrean Soran,Tamer M A Mohamed,Wei LiuPubMed 37123894
- International journal of molecular sciences 23:2022β-Klotho Promotes the Development of Intrauterine Adhesions via the PI3K/AKT Signaling Pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZizhen Guo,Yuqing Wang,Xiaoyang Wen,Xinxin Xu,Lei YanPubMed 36232594
- Cancer management and research 13:6377-63872021βKlotho Inhibits Cell Proliferation by Downregulating ELK4 and Predicts Favorable Prognosis in Prostate Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChanglin Mao,Wei Dong,Jiaju Lu,Zhao Zhang,Hongliang Wu,Armin Ghavamian,Dongbin Bi,Pei Gao,Zhao Liu,Sentai DingPubMed 34408497
- American journal of cancer research 11:1982-20042021βKlotho, a direct target of miR-206, contributes to the growth of hepatoblastoma through augmenting PI3K/Akt/mTOR signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTong Chen,Jianglong Chen,Xiuhao Zhao,Jing Zhou,Qingfeng Sheng,Linlin Zhu,Zhibao LvPubMed 34094665
- Life sciences 274:1193462021The potential role of plasma fibroblast growth factor 21 as a diagnostic biomarker for abdominal aortic aneurysm presence and development.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Xie,Liangying Yin,Dan Guo,Zixin Zhang,Yuexin Chen,Bao Liu,Wei Wang,Yuehong ZhengPubMed 33713667
- EBioMedicine 65:1032492021The KLB rs17618244 gene variant is associated with fibrosing MAFLD by promoting hepatic stellate cell activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNadia Panera,Marica Meroni,Miriam Longo,Annalisa Crudele,Luca Valenti,Emanuele Bellacchio,Luca Miele,Valentina D'Oria,Erika Paolini,Marco Maggioni,Anna Ludovica Fracanzani,Anna Alisi,Paola DongiovanniPubMed 33640795
- Journal of cerebral blood flow and metabolism : of 39:2355-23672019Robust RBM3 and β-klotho expression in developing neurons in the human brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTravis C Jackson et. al.PubMed 31566073
- Experimental neurology 317:34-502019FGF21 promotes functional recovery after hypoxic-ischemic brain injury in neonatal rats by activating the PI3K/Akt signaling pathway via FGFR1/β-klotho.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLixia Ye et. al.PubMed 30802446
- Developmental neuroscience 40:325-3362018Infants Uniquely Express High Levels of RBM3 and Other Cold-Adaptive Neuroprotectant Proteins in the Human Brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTravis C Jackson et. al.PubMed 30399610
- Journal of diabetes investigation 10:171-1812018Decreased placental and muscular expression of the fibroblast growth factor 19 in gestational diabetes mellitus.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDongyu Wang et. al.PubMed 29734515
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Expected |
| Gorilla | Predicted | Predicted |
| Macaque monkey | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Macaque monkey, Gorilla | Dilution info - | Notes - |
IHC-P
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Macaque monkey, Gorilla | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21 (By similarity).
Alternative names
Beta-klotho, BKL, BetaKlotho, Klotho beta-like protein, KLB
Recommended products
Rabbit Polyclonal KLB antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 98.98% PBS, 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Purification technique
- Affinity purification Immunogen
- Specificity
From Mar 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-KLB antibody (ab106794)
KLB contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
All lanes: Western blot - Anti-KLB antibody (ab106794) at 1 µg/mL
All lanes: HepG2 whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 119 kDa
Observed band size: 125 kDa, 39 kDa, 54 kDa
Exposure time: 2min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLB antibody (ab106794)
IHC image of KLB staining in Human normal stomach formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab106794, 5μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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