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Rabbit Recombinant Monoclonal KLB antibody. Suitable for WB and reacts with Human, Mouse samples.

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Images

Western blot - Anti-KLB antibody [EPR28904-537] (AB323480), expandable thumbnail
  • Western blot - Anti-KLB antibody [EPR28904-537] (AB323480), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Not recommended
Mouse
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Target data

Function

Contributes to the transcriptional repression of cholesterol 7-alpha-hydroxylase (CYP7A1), the rate-limiting enzyme in bile acid synthesis. Probably inactive as a glycosidase. Increases the ability of FGFR1 and FGFR4 to bind FGF21.

Additional Targets

Beta-klotho

Alternative names

Betakl, Beta-klotho, BKL, BetaKlotho, Klotho beta-like protein

Rabbit Recombinant Monoclonal KLB antibody. Suitable for WB and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28904-537
Purification technique
Affinity purification Protein A
Specificity

The observed molecular weight may vary from the predicted molecular weight due to post-translational modifications.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Western blot - Anti-KLB antibody [EPR28904-537] (ab323480), expandable thumbnail

    Western blot - Anti-KLB antibody [EPR28904-537] (ab323480)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: HeLa.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26505219, PMID: 22696219).

    The observed molecular weight may vary from the predicted molecular weight due to post-translational modifications.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-KLB antibody [EPR28904-537] (ab323480) at 1/1000 dilution

    Lane 1: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 2: Huh7 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 50 µg

    Lane 3: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 140-150 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-KLB antibody [EPR28904-537] (ab323480), expandable thumbnail

    Western blot - Anti-KLB antibody [EPR28904-537] (ab323480)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: kidney, skeletal muscle, stomach (PMID: 11044614).

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 26505219, PMID: 22696219).

    The observed molecular weight may vary from the predicted molecular weight due to post-translational modifications.

    The identity of the lower MW band at approximately 37 kDa (in lanes 2, 4) is unknown.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-KLB antibody [EPR28904-537] (ab323480) at 1/1000 dilution

    Lane 1: Mouse white fat tissue lysate at 50 µg

    Lane 2: Mouse liver tissue lysate at 50 µg

    Lane 3: Mouse kidney tissue lysate at 50 µg

    Lane 4: Mouse skeletal muscle tissue lysate  at 50 µg

    Lane 5: Mouse stomach tissue lysate at 50 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 140-150 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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