Rabbit Recombinant Monoclonal KLC1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Images
![Western blot - Anti-KLC1 antibody [EPR12441(B)] (AB174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--western-blot-img146701.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KLC1 antibody [EPR12441(B)] (AB174273)")
![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (AB174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunocytochemistry-immunofluorescence-img14161.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (AB174273)")
![Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (AB174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunoprecipitation-img53254.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (AB174273)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (AB174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78360.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (AB174273)")
![Western blot - Anti-KLC1 antibody [EPR12441(B)] (AB174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--western-blot-img115486.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KLC1 antibody [EPR12441(B)] (AB174273)")
Publications
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- Frontiers in aging neuroscience 14:10494182022Time-dependent alterations in the rat nigrostriatal system after intrastriatal injection of fibrils formed by α-Syn and tau fragments.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoman Yang,Jialing Wang,Weiqi Zeng,Xiaoqian Zhang,Xiaomei Yang,Yu Xu,Yan Xu,Xuebing CaoPubMed 36518823
- Cell death and differentiation 27:2383-24012020Drp1 overexpression induces desmin disassembling and drives kinesin-1 activation promoting mitochondrial trafficking in skeletal muscle.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMatteo Giovarelli et. al.PubMed 32042098
- EBioMedicine 45:92-1072019A role for kinesin-1 subunits KIF5B/KLC1 in regulating epithelial mesenchymal plasticity in breast tumorigenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlaa Moamer et. al.PubMed 31204277
- Cell reports 26:94-107.e72019Microtubule-Driven Stress Granule Dynamics Regulate Inhibitory Immune Checkpoint Expression in T Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDon-Marc Franchini,Olivia Lanvin,Marie Tosolini,Emilie Patras de Campaigno,Anne Cammas,Sarah Péricart,Clara-Maria Scarlata,Morgane Lebras,Cédric Rossi,Laetitia Ligat,Fréderic Pont,Paola B Arimondo,Camille Laurent,Maha Ayyoub,Fabien Despas,Maryse Lapeyre-Mestre,Stefania Millevoi,Jean-Jacques FourniéPubMed 30605689
- Cell reports 20:2792-27992017Localized Phosphorylation of a Kinesin-1 Adaptor by a Capsid-Associated Kinase Regulates HIV-1 Motility and Uncoating.Applications:Unspecified applicationReactive species:Unspecified reactive speciesViacheslav Malikov,Mojgan H NaghaviPubMed 28930676
- Development (Cambridge, England) 142:218-282014Nesprins anchor kinesin-1 motors to the nucleus to drive nuclear distribution in muscle cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeredith H Wilson,Erika L F HolzbaurPubMed 25516977
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/100 | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/500 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10 - 1/100 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport (PubMed:21385839). The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity (By similarity).
Alternative names
KLC, KNS2, KLC1, Kinesin light chain 1, KLC 1
Recommended products
Rabbit Recombinant Monoclonal KLC1 antibody. Suitable for IHC-P, IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 6 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9% PBS, 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR12441(B)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
![Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--western-blot-img146701.jpg "Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Lanes 1-3: Merged signal (red and green). Green - ab174273 observed at 65 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.ab174273 Anti-KLC1 antibody [EPR12441(B)] was shown to specifically react with KLC1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line Human KLC1 knockout HeLa cell line ab265284 (knockout cell lysate Human KLC1 knockout HeLa cell lysate ab257496) was used. Wild-type and KLC1 knockout samples were subjected to SDS-PAGE. ab174273 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: KLC1 knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human KLC1 knockout HeLa cell line (Human KLC1 knockout HeLa cell line ab265284)
Lane 3: U-87 MG cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 65 kDa
Observed band size: 65 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunocytochemistry-immunofluorescence-img14161.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) labelling KLC1 with purified ab174273 at 1/500. Cells were fixed with 100% methanol. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077). Nuclei counterstained with DAPI (blue).
Control: PBS only
![Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunoprecipitation-img53254.jpg "Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Western blot analysis on immunoprecipitation pellet from SH-SY5Y cell lysate using ab174273 at 1/10 dilution.
All lanes: Immunoprecipitation - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Predicted band size: 65 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img78360.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling KLC1 with ab174273 at 1/50 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
![Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--western-blot-img115486.jpg "Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
All lanes: Western blot - Anti-KLC1 antibody [EPR12441(B)] (ab174273) at 1/1000 dilution
Lane 1: U87-MG cell lysate at 10 µg
Lane 2: 293T cell lysate at 10 µg
Lane 3: SH-SY5Y cell lysate at 10 µg
Lane 4: HeLa cell lysate at 10 µg
Predicted band size: 65 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunocytochemistry-immunofluorescence-img100177.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Immunofluorescent analysis of U87-MG cells labeling KLC1 with ab174273 at 1/50 dilution.
![Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--flow-cytometry-intracellular-img26364.jpg "Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)
Intracellular flow cytometric analysis of permeabilized HeLa cells labeling KLC1with ab174273 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
![Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--immunocytochemistry-immunofluorescence-img100177.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
![Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273), expandable thumbnail](https://content.abcam.com/products/images/klc1-antibody-epr12441b-ab174273--flow-cytometry-intracellular-img26364.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KLC1 antibody [EPR12441(B)] (ab174273)")
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