AB180523

Anti-KLC3 antibody [EPR11149(2)]

RabMAb
Recombinant
What is this?

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(6 Publications)

Rabbit Recombinant Monoclonal KLC3 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 6 publications.

View Alternative Names

KLC2, KLC2L, KLC3, Kinesin light chain 3, KLC2-like, kinesin light chain 2

5 Images

Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed)labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC)at 1/75 dilution was used as the secondary antibody.Rabbit monoclonal IgG was used as the isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot® : Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.

All lanes:

Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (ab180523)

Predicted band size: 55 kDa

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Supplier Data

Western blot - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

All lanes:

Western blot - Anti-KLC3 antibody [EPR11149(2)] (ab180523) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

U-87 MG cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11149(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/1000", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/10", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa (By similarity). May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece (By similarity).

See full target information KLC3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Oncology reports 53: PubMed40242978

2025

KLC3 activates PI3K/AKT signaling and promotes ovarian cancer cell proliferation and migration through COL3A1.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Yang,Rongxia He,Xiaoling Zhang,Xing Wang,Min Liu,Xiaolong Liu,Yulan Li

Journal of nanobiotechnology 22:548 PubMed39238028

2024

A nanoscale visual exploration of the pathogenic effects of bacterial extracellular vesicles on host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Minjae Kang,Min Jeong Kim,Dokyung Jeong,Hyung-Jun Lim,Ga-Eun Go,Uidon Jeong,Eunyoung Moon,Hee-Seok Kweon,Nae-Gyu Kang,Seung Jin Hwang,Sung Hun Youn,Bo Kyoung Hwang,Doory Kim

Journal of the American Society of Nephrology : JASN 33:1726-1741 PubMed35961787

2022

KLC3 Regulates Ciliary Trafficking and Cyst Progression in CILK1 Deficiency-Related Polycystic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Gyuyeong Rah,Hwayeon Cha,Joohee Kim,Jieun Song,Hyunho Kim,Yun Kyu Oh,Curie Ahn,Minyong Kang,Jongmin Kim,Kyung Hyun Yoo,Min Jung Kim,Hyuk Wan Ko,Je Yeong Ko,Jong Hoon Park

Structure (London, England : 1993) 29:810-822.e3 PubMed34143977

2021

Super-resolution imaging reveals cytoskeleton-dependent organelle rearrangement within platelets at intermediate stages of maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Seokran Go,Dokyung Jeong,Jinkyoung Chung,Geun-Ho Kim,Jaewoo Song,Eunyoung Moon,Yang Hoon Huh,Doory Kim

Scientific reports 11:10511 PubMed34006947

2021

Super-resolution imaging of platelet-activation process and its quantitative analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Jinkyoung Chung,Dokyung Jeong,Geun-Ho Kim,Seokran Go,Jaewoo Song,Eunyoung Moon,Yang Hoon Huh,Doory Kim

Scientific reports 7:16474 PubMed29184084

2017

The flexibility and dynamics of the tubules in the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Pantelis Georgiades,Victoria J Allan,Graham D Wright,Philip G Woodman,Parinya Udommai,Manloeng A Chung,Thomas A Waigh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLC3 antibody [EPR11149(2)]

Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Western blot - Anti-KLC3 antibody [EPR11149(2)] (AB180523)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise