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AB250220

Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal KLC3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

KLC2, KLC2L, KLC3, Kinesin light chain 3, KLC2-like, kinesin light chain 2

5 Images
Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)

This data was developed using ab180523, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)

This data was developed using ab180523, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)

This data was developed using ab180523, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)
  • IP

Supplier Data

Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)

This data was developed using ab180523, the same antibody clone in a different buffer formulation.

Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot® : Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.

All lanes:

Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (<a href='/en-us/products/primary-antibodies/klc3-antibody-epr111492-ab180523'>ab180523</a>)

Predicted band size: 55 kDa

false

Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)
  • WB

Supplier Data

Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)

This data was developed using ab180523, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-KLC3 antibody [EPR11149(2)] (<a href='/en-us/products/primary-antibodies/klc3-antibody-epr111492-ab180523'>ab180523</a>) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

U-87 MG cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

BxPC-3 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11149(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, Flow Cyt (Intra), ICC/IF, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab250220 is the carrier-free version of ab180523.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa (By similarity). May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece (By similarity).
See full target information KLC3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

OncoTargets and therapy 13:13315-13327 PubMed33408482

2020

Circ_0001175 Promotes Hepatocellular Carcinoma Cell Proliferation and Metastasis by Regulating miR-130a-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Liheng Li,Ke He,Siliang Chen,Wenjiang Wei,Zuofu Tian,Yinghong Tang,Chengjiang Xiao,Guoan Xiang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com