Rabbit Recombinant Monoclonal KLC3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Images
![Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--western-blot-img103845.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96073.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)")
![Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--immunoprecipitation-img68981.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)")
![Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--immunocytochemistry-immunofluorescence-img1985.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)")
![Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--flow-cytometry-intracellular-img51668.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (AB250220)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | WB | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Target data
Function
Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Plays a role during spermiogenesis in the development of the sperm tail midpiece and in the normal function of spermatozoa (By similarity). May play a role in the formation of the mitochondrial sheath formation in the developing spermatid midpiece (By similarity).
Alternative names
KLC2, KLC2L, KLC3, Kinesin light chain 3, KLC2-like, kinesin light chain 2
Recommended products
Rabbit Recombinant Monoclonal KLC3 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra), IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR11149(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250220 is the carrier-free version of Anti-KLC3 antibody [EPR11149(2)] ab180523.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--western-blot-img103845.jpg "Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)")
Western blot - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
This data was developed using Anti-KLC3 antibody [EPR11149(2)] ab180523, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-KLC3 antibody [EPR11149(2)] (Anti-KLC3 antibody [EPR11149(2)] ab180523) at 1/50000 dilution
Lane 1: Jurkat cell lysate at 20 µg
Lane 2: U-87 MG cell lysate at 20 µg
Lane 3: HepG2 cell lysate at 20 µg
Lane 4: BxPC-3 cell lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 55 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img96073.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
This data was developed using Anti-KLC3 antibody [EPR11149(2)] ab180523, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling KLC3 with Anti-KLC3 antibody [EPR11149(2)] ab180523 at 1/250 dilution followed by ImmunoHistoprobe (Ready to use) HRP Polymer for Rabbit IgG secondary and counter stained with Hematoxylin. Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--immunoprecipitation-img68981.jpg "Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)")
Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
This data was developed using Anti-KLC3 antibody [EPR11149(2)] ab180523, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from BxPC-3 cell lysate immunoprecipitated using Anti-KLC3 antibody [EPR11149(2)] ab180523 at 1/40 dilution (lane 1). Negative control (lane 2). Rabbit TrueBlot®:Anti-Rabbit IgG HRP at 1/1500 dilution was used as secondary.
All lanes: Immunoprecipitation - Anti-KLC3 antibody [EPR11149(2)] (Anti-KLC3 antibody [EPR11149(2)] ab180523)
Predicted band size: 55 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--immunocytochemistry-immunofluorescence-img1985.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)")
Immunocytochemistry/ Immunofluorescence - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
This data was developed using Anti-KLC3 antibody [EPR11149(2)] ab180523, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HepG2 cells (4% paraformaldehyde-fixed) labeling KLC3 with Anti-KLC3 antibody [EPR11149(2)] ab180523 at 1/1000 dilution followed by Goat anti-rabbit IgG (Dylight 555) at 1/250 dilution and counterstained with DAPI.
![Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220), expandable thumbnail](https://content.abcam.com/products/images/klc3-antibody-epr111492-bsa-and-azide-free-ab250220--flow-cytometry-intracellular-img51668.jpg "Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)")
Flow Cytometry (Intracellular) - Anti-KLC3 antibody [EPR11149(2)] - BSA and Azide free (ab250220)
This data was developed using Anti-KLC3 antibody [EPR11149(2)] ab180523, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of Jurkat cells (2% paraformaldehyde-fixed) labeling KLC3 with Anti-KLC3 antibody [EPR11149(2)] ab180523 at 1/10 dilution. Goat anti-rabbit IgG (FITC) at 1/75 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control.
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