AB73537

Anti-KLF10 antibody

Name: Anti-KLF10 antibody (ab73537) | Abcam
Brand: Abcam Limited
SKU: AB73537
Price: 190 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(15 Publications)

Rabbit Polyclonal KLF10 antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 15 publications.

View Alternative Names

TIEG, TIEG1, KLF10, Krueppel-like factor 10, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1, TGFB-inducible early growth response protein 1, TIEG-1

1 Images

Project

Western blot - Anti-KLF10 antibody (AB73537)

All lanes:

Western blot - Anti-KLF10 antibody (ab73537) at 1 µg/mL

Lane 1:

Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg

Lane 2:

Liver (Mouse) Tissue Lysate at 10 µg

Lane 3:

Human skeletal muscle tissue lysate - total protein (<a href='/en-us/products/unavailable/human-skeletal-muscle-tissue-lysate-total-protein-ab29330'>ab29330</a>) at 10 µg

Lane 4:

Testis (Mouse) Tissue Lysate at 10 µg

Lane 5:

Thymus (Rat) Tissue Lysate at 10 µg

Lane 6:

Ramos (Human Burkitt's lymphoma cell line) Whole Cell Lysate at 10 µg

Lane 7:

Human brain tissue lysate - total protein (<a href='/en-us/products/unavailable/human-brain-tissue-lysate-total-protein-ab29466'>ab29466</a>) at 10 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa,85 kDa

false

Exposure time: 12min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF10 also known as TIEG1 is a transcription factor belonging to the Kruppel-like factor family. It weighs approximately 54 kDa and is expressed in various tissues including bone liver and muscle. KLF10 plays an important role in regulating gene expression influencing numerous cellular processes such as proliferation differentiation and apoptosis. Its expression levels often respond to external stimuli like transforming growth factor-beta (TGF-β) reflecting its versatile function in cellular responses.

Biological function summary

KLF10 affects several pathways through its interactions with other proteins and DNA elements. As a member of the human transcription machinery it acts as a coregulator often partnering with SMAD proteins to modulate gene expression in TGF-β signaling. KLF10 influences cellular activities by regulating genes involved in tissue development and repair making it integral to maintaining cellular homeostasis and function across numerous organ systems.

Pathways

This protein integrates into key signaling cascades most notably TGF-β and bone morphogenetic protein (BMP) signaling pathways. In TGF-β signaling KLF10 works closely with SMAD4 to regulate genes that control cellular growth and differentiation. This partnership is important for transmitting signals that govern cell fate decisions often influencing how cells respond to their immediate environment and driving critical cellular processes.

KLF10 links to conditions such as osteoporosis and cancer. Alterations in its expression or function can disrupt normal cellular processes contributing to the pathogenesis of these diseases. For example in osteoporosis KLF10's role in bone remodeling and maintenance becomes dysregulated affecting bone density and strength. In cancer its interactions with other proteins like SMAD4 and p53 can influence cell cycle control and tumor suppression highlighting its dual role in both health and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.

See full target information KLF10

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Publications (15)

Recent publications for all applications. Explore the full list and refine your search

Journal of biomedical science 30:39 PubMed37308977

2023

Krüppel-like factor 10 modulates stem cell phenotypes of pancreatic adenocarcinoma by transcriptionally regulating notch receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chih Tsai,Kung Hung Cheng,Shih Sheng Jiang,John R Hawse,Shun En Chuang,Su Liang Chen,Tze-Sing Huang,Hui-Ju Ch'ang

EMBO reports 23:e54229 PubMed35492028

2022

KLF10 promotes nonalcoholic steatohepatitis progression through transcriptional activation of zDHHC7.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Yang,Lijing Jia,Jiaqing Xiang,Guangyan Yang,Shanhu Qiu,Lin Kang,Peilin Zheng,Zhen Liang,Yan Lu

Circulation research 130:1662-1681 PubMed35440172

2022

Perivascular Fibrosis Is Mediated by a KLF10-IL-9 Signaling Axis in CD4+ T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rulin Zhuang,Jingshu Chen,Henry S Cheng,Carmel Assa,Anurag Jamaiyar,Arvind K Pandey,Daniel Pérez-Cremades,Bofang Zhang,Aspasia Tzani,Akm Khyrul Wara,Jorge Plutzky,Victor Barrera,Preetida Bhetariya,Richard N Mitchell,Zhongmin Liu,Mark W Feinberg

Laboratory investigation; a journal of technical methods and pathology 102:826-837 PubMed35332261

2022

Bone mesenchymal stem cell-derived extracellular vesicles containing NORAD promote osteosarcoma by miR-30c-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Hongtao He,Muliang Ding,Tao Li,Wenzhi Zhao,Lu Zhang,Peng Yin,Wei Zhang

Frontiers in cell and developmental biology 9:710721 PubMed34805140

2021

Effect of Photodynamic Therapy on Gemcitabine-Resistant Cholangiocarcinoma and Through KLF10 and EGFR.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yang,Jigang Li,Lei Yao,Lile Wu

Bioengineered 13:1779-1790 PubMed34713769

2021

Kruppel like factor 10 up-regulates PDZ and LIM domain containing protein 2 via nuclear factor kappa-B pathway to inhibit proliferation and inflammatory of fibroblastoid synovial cell in rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Shan Wang,Xuwen Zha,Shengting Ruan,Shoulin Yao,Xiaoyu Zhang

Experimental & molecular medicine 53:1623-1635 PubMed34702956

2021

Upregulating sirtuin 6 ameliorates glycolysis, EMT and distant metastasis of pancreatic adenocarcinoma with krüppel-like factor 10 deficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Yi-Chih Tsai,Su-Liang Chen,Shu-Ling Peng,Ya-Li Tsai,Zuong-Ming Chang,Vincent Hung-Shu Chang,Hui-Ju Ch'ang

Cell reports 33:108550 PubMed33378664

2020

KLF10 Deficiency in CD4 T Cells Triggers Obesity, Insulin Resistance, and Fatty Liver.

Applications

Unspecified application

Species

Unspecified reactive species

Akm Khyrul Wara,Shijia Wang,Chun Wu,Fang Fang,Stefan Haemmig,Brittany N Weber,Ceren O Aydogan,Yevgenia Tesmenitsky,Hassan Aliakbarian,John R Hawse,Malayannan Subramaniam,Lei Zhao,Peter T Sage,Ali Tavakkoli,Amanda Garza,Lydia Lynch,Alexander S Banks,Mark W Feinberg

Theranostics 10:7787-7811 PubMed32685020

2020

-derived miRNAs suppress vascular remodeling through regulating OTUD7B/KLF4/NMHC IIA axis.

Applications

Unspecified application

Species

Unspecified reactive species

Gao-Shan Yang,Bin Zheng,Yan Qin,Jing Zhou,Zhan Yang,Xin-Hua Zhang,Hong-Ye Zhao,Hao-Jie Yang,Jin-Kun Wen

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 38:1987-1995 PubMed32144802

2020

KLF10 is a modulatory factor of chondrocyte hypertrophy in developing skeleton.

Applications

IHC-Fr

Species

Mouse

Jong Min Lee,Ji-Yun Ko,Jeong-Won Park,Woon Kyu Lee,Sun U Song,Gun-Il Im

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLF10 antibody

Western blot - Anti-KLF10 antibody (AB73537)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (15)

Product promise