AB184182

Anti-KLF10 antibody [EPR12102(2)]

RabMAb
Recombinant
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(7 Publications )

Rabbit Recombinant Monoclonal KLF10 antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

2 Images

Supplier Data

Western blot - Anti-KLF10 antibody [EPR12102(2)] (AB184182)

All lanes:

Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182) at 1/2000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

K652 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-KLF10 antibody [EPR12102(2)] (AB184182)

All lanes:

Western blot - Anti-KLF10 antibody [EPR12102(2)] (ab184182) at 1/10000 dilution

Lane 1:

HepG2 cell lysate at 20 µg

Lane 2:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12102(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF10 also known as TIEG1 is a transcription factor belonging to the Kruppel-like factor family. It weighs approximately 54 kDa and is expressed in various tissues including bone liver and muscle. KLF10 plays an important role in regulating gene expression influencing numerous cellular processes such as proliferation differentiation and apoptosis. Its expression levels often respond to external stimuli like transforming growth factor-beta (TGF-β) reflecting its versatile function in cellular responses.

Biological function summary

KLF10 affects several pathways through its interactions with other proteins and DNA elements. As a member of the human transcription machinery it acts as a coregulator often partnering with SMAD proteins to modulate gene expression in TGF-β signaling. KLF10 influences cellular activities by regulating genes involved in tissue development and repair making it integral to maintaining cellular homeostasis and function across numerous organ systems.

Pathways

This protein integrates into key signaling cascades most notably TGF-β and bone morphogenetic protein (BMP) signaling pathways. In TGF-β signaling KLF10 works closely with SMAD4 to regulate genes that control cellular growth and differentiation. This partnership is important for transmitting signals that govern cell fate decisions often influencing how cells respond to their immediate environment and driving critical cellular processes.

KLF10 links to conditions such as osteoporosis and cancer. Alterations in its expression or function can disrupt normal cellular processes contributing to the pathogenesis of these diseases. For example in osteoporosis KLF10's role in bone remodeling and maintenance becomes dysregulated affecting bone density and strength. In cancer its interactions with other proteins like SMAD4 and p53 can influence cell cycle control and tumor suppression highlighting its dual role in both health and disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor which binds to the consensus sequence 5'-GGTGTG-3'. Plays a role in the regulation of the circadian clock; binds to the GC box sequence in the promoter of the core clock component ARTNL/BMAL1 and represses its transcriptional activity. Regulates the circadian expression of genes involved in lipogenesis, gluconeogenesis, and glycolysis in the liver. Represses the expression of PCK2, a rate-limiting step enzyme of gluconeogenesis (By similarity). May play a role in the cell cycle regulation.

See full target information KLF10

Alternative Names

TIEG, TIEG1, KLF10, Krueppel-like factor 10, EGR-alpha, Transforming growth factor-beta-inducible early growth response protein 1, TGFB-inducible early growth response protein 1, TIEG-1

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Publications (7)

Recent publications for all applications. Explore the full listand refine your search

The FEBS journal 291:4714-4731 PubMed39206622

2024

PELO regulates erythroid differentiation through interaction with MYC to upregulate KLF10.

Applications

Unspecified application

Species

Unspecified reactive species

Jinglan Hao,Guiqin Han,Xin Liang,Yongtong Ruan,Chen Huang,Naer Sa,Hang Hu,Bixi Hu,Zhongqi Li,Kai Zhang,Ping Gao,Xiaoming Dong

Translational lung cancer research 12:2520-2537 PubMed38205211

2024

Exosomal ITGB6 from dormant lung adenocarcinoma cells activates cancer-associated fibroblasts by KLF10 positive feedback loop and the TGF-β pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Feng,Xianling Liu,Juanjuan Xiang,Jiaqi Xu,Na Yin,Lujuan Wang,Chaoyuan Liu,Yuyao Liu,Tiantian Zhao,Zengyi Zhao,Yawen Gao

Polish journal of pathology : official journal of the Polish Society of Pathologists 74:182-193 PubMed37955537

2023

S100A16 cooperates with DEPDC1 to promote the progression and angiogenesis of nephroblastoma through PI3K/Akt/mTOR pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Geng Geng,Yongtao Xu,Qingfang Li,Qinghao Li,Lili Yuan,Mengyao Dong,Ming Ming

Cell death discovery 9:82 PubMed36878898

2023

Downregulation of KLF10 contributes to the regeneration of survived renal tubular cells in cisplatin-induced acute kidney injury via ZBTB7A-KLF10-PTEN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Zhang,Siyu Bao,Daxi Wang,Wei Lu,Sujuan Xu,Weiran Zhou,Xiaoyan Wang,Xialian Xu,Xiaoqiang Ding,Shuan Zhao

Oncology letters 23:175 PubMed35497935

2022

KLF10 upregulates ACSM3 via the PI3K/Akt signaling pathway to inhibit the malignant progression of melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhirong Zhao,Yuanchang Zhan,Li Jing,Huali Zhai

Aging 13:14399-14415 PubMed34031263

2021

MicroRNA-126 engineered muscle-derived stem cells attenuates cavernosa injury-induced erectile dysfunction in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zihao Zou,Muyuan Chai,Feixiang Guo,Xin Fu,Yu Lan,Shuqi Cao,Jianan Liu,Long Tian,Geng An

Cancer research 77:2387-2400 PubMed28249899

2017

Krüppel-like Transcription Factor KLF10 Suppresses TGFβ-Induced Epithelial-to-Mesenchymal Transition via a Negative Feedback Mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Vivek Kumar Mishra,Malayannan Subramaniam,Vijayalakshmi Kari,Kevin S Pitel,Simon J Baumgart,Ryan M Naylor,Sankari Nagarajan,Florian Wegwitz,Volker Ellenrieder,John R Hawse,Steven A Johnsen

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLF10 antibody [EPR12102(2)]

Western blot - Anti-KLF10 antibody [EPR12102(2)] (AB184182)

Western blot - Anti-KLF10 antibody [EPR12102(2)] (AB184182)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (7)

Product promise