AB129459

Anti-KLF12 antibody

Name: Anti-KLF12 antibody (ab129459) | Abcam
Brand: Abcam Limited
SKU: AB129459
Rating: 4 (1 reviews)

(1 Review)

(13 Publications)

Rabbit Polyclonal KLF12 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Synthetic Peptide within Human KLF12 aa 1-100.

View Alternative Names

AP2REP, HSPC122, KLF12, Krueppel-like factor 12, Transcriptional repressor AP-2rep

2 Images

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Immunoprecipitation - Anti-KLF12 antibody (AB129459)

Detection of KLF12 by Western Blot of Immunoprecipitates.

ab129459 at 0.4µg/ml staining KLF12 in Jurkat whole cell lysate immunoprecipitated using ab129459 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-KLF12 antibody (ab129459)

Predicted band size: 44 kDa

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Western blot - Anti-KLF12 antibody (AB129459)

All lanes:

Western blot - Anti-KLF12 antibody (ab129459) at 0.1 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

HeLa whole cell lysate at 50 µg

Predicted band size: 44 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human KLF12 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9Y4X4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab129459 is affinity purified using an epitope specific to KLF12 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF12 also known as Krüppel-like factor 12 is a transcription factor with a mass of approximately 37 kDa. This protein is expressed in diverse tissues including the liver heart and brain. KLF12 modulates gene expression by binding to specific DNA sequences with its zinc finger domains. The factor plays an important role in transcription regulation by interacting with promoter regions of target genes.

Biological function summary

KLF12 influences cell differentiation proliferation and apoptosis. It operates as part of transcriptional complexes often interacting with co-regulators such as histone deacetylases. The protein controls the expression of critical genes involved in cellular growth and maintenance exerting broad impacts on cellular phenotype. KLF12's interaction with co-repressors alters chromatin structure influencing transcriptional landscapes.

Pathways

KLF12 regulates the Wnt signaling pathway which is significant for controlling cell fate and migration. KLF12 interacts with components like beta-catenin altering the pathway's output and affecting cellular responses to developmental signals. Additionally KLF12 is linked to the TGF-beta signaling pathway influencing related proteins and impacting cellular processes such as proliferation and differentiation.

KLF12 shows strong associations with cancer particularly colorectal cancer. It interacts with proteins like E-cadherin affecting the epithelial-to-mesenchymal transition an important step in cancer metastasis. Furthermore KLF12 also connects to neurodegenerative disorders influencing factors linked to neural integrity and function underlining its potential role in pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.

See full target information KLF12

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of women's health 17:1165-1174 PubMed40308269

2025

MiR-484 Regulates the IL-6/STAT3 Signaling Pathway by Down-Regulating KLF12 to Inhibit the Malignant Progression of Cervical Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Keyong Bao,Xue Zhang,Lihong Bao,Xiaoyu Tao,Li Zhang,Dong Wang

Molecular oncology 17:2659-2674 PubMed37606530

2023

KLF12 transcriptionally regulates PD-L1 expression in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaohui Pan,Wenxin Zhang,Longsheng Wang,Hongjie Guo,Mingming Zheng,Honghai Wu,Qinjie Weng,Qiaojun He,Ling Ding,Bo Yang

Journal for immunotherapy of cancer 11: PubMed37586772

2023

KLF12 overcomes anti-PD-1 resistance by reducing galectin-1 in cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yujia Zheng,Hao Zhang,Chu Xiao,Ziqin Deng,Tao Fan,Bo Zheng,Chunxiang Li,Jie He

Cell death & disease 14:313 PubMed37156774

2023

KLF12 promotes the proliferation of breast cancer cells by reducing the transcription of p21 in a p53-dependent and p53-independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Yanan Li,Shujing Li,Xiaoxia Shi,Zhiqiang Xin,Yuxi Yang,Binggong Zhao,Yvlin Li,Linlin Lv,Ping Ren,Huijian Wu

Journal of bone oncology 37:100455 PubMed36276300

2022

Silencing of circCYP51A1 represses cell progression and glycolysis by regulating miR-490-3p/KLF12 axis in osteosarcoma under hypoxia.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Yang,Zhiwei Liu,Ben Liu,Lishan Sun

Genetics and molecular biology 45:e20210370 PubMed36121916

2022

Identification of super-enhancer-associated transcription factors regulating glucose metabolism in poorly differentiated thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Kun Liu,Yongrui Du,Hui Li,Xuexia Lin

Open medicine (Warsaw, Poland) 16:1415-1427 PubMed34616917

2021

circ_0000467 promotes the proliferation, metastasis, and angiogenesis in colorectal cancer cells through regulating KLF12 expression by sponging miR-4766-5p.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Chen,Chen Wu,Liang Luo,Yuan Wang,Fangxing Peng

Cell & bioscience 11:68 PubMed33820555

2021

Long non-coding RNA NEAT1 transported by extracellular vesicles contributes to breast cancer development by sponging microRNA-141-3p and regulating KLF12.

Applications

Unspecified application

Species

Unspecified reactive species

DaoPing Zhou,Juan Gu,YuePing Wang,HuaiGuo Wu,Wei Cheng,QingPing Wang,GuoPei Zheng,XueDong Wang

Cancer management and research 13:2717-2731 PubMed33790646

2021

Downregulation of Circ_0071589 Suppresses Cisplatin Resistance in Colorectal Cancer by Regulating the MiR-526b-3p/KLF12 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Weitong Zhang,Zhenfen Wang,Guohao Cai,Ping Huang

Journal of bioenergetics and biomembranes 53:235-245 PubMed33604734

2021

Long non-coding RNA AGAP2-AS1 promotes proliferation and metastasis in papillary thyroid cancer by miR-628-5p/KLF12 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chongwen Xu,Yuan Shao,Junsong Liu,Xiaobao Yao,Fang Quan,Qian Zhao,Ruimin Zhao,Bo Kou,Honghui Li,Peng Han,Xuan Wang,Yanxia Bai,Zhiwei Zheng,Shaoqiang Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLF12 antibody

Immunoprecipitation - Anti-KLF12 antibody (AB129459)

Western blot - Anti-KLF12 antibody (AB129459)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

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