AB221602

Anti-KLF12 antibody

Name: Anti-KLF12 antibody (ab221602) | Abcam
Brand: Abcam Limited
SKU: AB221602

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(3 Publications)

Rabbit Polyclonal KLF12 antibody. Suitable for ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLF12 aa 1-100.

View Alternative Names

AP2REP, HSPC122, KLF12, Krueppel-like factor 12, Transcriptional repressor AP-2rep

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-KLF12 antibody (AB221602)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLF12 antibody (AB221602)

PFA-fixed, Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells stained for KLF12 (green) using ab221602 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KLF12 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF12 also known as Krüppel-like factor 12 is a transcription factor with a mass of approximately 37 kDa. This protein is expressed in diverse tissues including the liver heart and brain. KLF12 modulates gene expression by binding to specific DNA sequences with its zinc finger domains. The factor plays an important role in transcription regulation by interacting with promoter regions of target genes.

Biological function summary

KLF12 influences cell differentiation proliferation and apoptosis. It operates as part of transcriptional complexes often interacting with co-regulators such as histone deacetylases. The protein controls the expression of critical genes involved in cellular growth and maintenance exerting broad impacts on cellular phenotype. KLF12's interaction with co-repressors alters chromatin structure influencing transcriptional landscapes.

Pathways

KLF12 regulates the Wnt signaling pathway which is significant for controlling cell fate and migration. KLF12 interacts with components like beta-catenin altering the pathway's output and affecting cellular responses to developmental signals. Additionally KLF12 is linked to the TGF-beta signaling pathway influencing related proteins and impacting cellular processes such as proliferation and differentiation.

KLF12 shows strong associations with cancer particularly colorectal cancer. It interacts with proteins like E-cadherin affecting the epithelial-to-mesenchymal transition an important step in cancer metastasis. Furthermore KLF12 also connects to neurodegenerative disorders influencing factors linked to neural integrity and function underlining its potential role in pathological processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Confers strong transcriptional repression to the AP-2-alpha gene. Binds to a regulatory element (A32) in the AP-2-alpha gene promoter.

See full target information KLF12

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 15:2526 PubMed38514666

2024

Trimethylamine N-oxide impairs β-cell function and glucose tolerance.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Kong,Qijin Zhao,Xiaojing Jiang,Jinping Hu,Qian Jiang,Li Sheng,Xiaohong Peng,Shusen Wang,Yibing Chen,Yanjun Wan,Shaocong Hou,Xingfeng Liu,Chunxiao Ma,Yan Li,Li Quan,Liangyi Chen,Bing Cui,Pingping Li

Cancer management and research 13:3789-3801 PubMed34007212

2021

Depletion of Circular RNA circ_CORO1C Suppresses Gastric Cancer Development by Modulating miR-138-5p/KLF12 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongqiang Fan,Min Liu,Anquan Liu,Nailing Cui,Zhimei Chen,Qian Yang,Aihua Su

Oncology letters 19:2475-2480 PubMed32194747

2020

Long non-coding RNA ELF3-antisense RNA 1 promotes osteosarcoma cell proliferation by upregulating Kruppel-like factor 12 potentially via methylation of the microRNA-205 gene.

Applications

Unspecified application

Species

Unspecified reactive species

Jianmin Yuan,Jianhui Kang,Min Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLF12 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KLF12 antibody (AB221602)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise