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Rabbit Multiclonal KLF17 antibody. Suitable for WB and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLF17.

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Images

Western blot - Anti-KLF17 antibody [RP23040191] (AB308133), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.4
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS

Form

Liquid

Clonality

Multiclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human KLF17. Database link Q5JT82

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Mouse
Tested
Rat
Tested

Tested
Tested

Species

Mouse

Dilution info

1-2 µg/mL

Notes

-

Species

Rat

Dilution info

1-2 µg/mL

Notes

-

Target data

Function

Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development (By similarity).

Alternative names

Recommended products

Rabbit Multiclonal KLF17 antibody. Suitable for WB and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLF17.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogen
  • Recombinant Full Length Protein corresponding to Human KLF17. Database link Q5JT82
Clone number

RP23040191

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.

Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KLF17 also known as Krüppel-like factor 17 is a zinc-finger transcription factor with a mass of approximately 35 kDa. This protein plays a role in DNA-binding and regulation of transcription. It regulates the expression of target genes by binding to GC-rich regions. Researchers have observed KLF17 expression in various tissues with notable levels in the reproductive organs such as the ovary and testis as well as in embryonic tissues which indicates its involvement in reproductive biology and development.

Biological function summary

KLF17 influences cellular processes by modulating the expression of genes involved in cell differentiation and proliferation. It functions primarily as a transcriptional repressor acting independently and not as part of a larger multiprotein complex. Its regulatory role affects key cellular functions contributing to the control of epithelial-mesenchymal transition (EMT) which is critical during embryogenesis and cancer metastasis.

Pathways

KLF17 plays a significant role in the TGF-β signaling pathway which is important in numerous cellular functions such as growth and differentiation. KLF17 interacts with other transcription factors like SNAIL and TWIST influencing pathways that control EMT and metastasis. This regulation within TGF-β signaling highlights KLF17's role in maintaining cellular architecture and regulating processes critical to tissue development and disease progression.

Associated diseases and disorders

KLF17 shows a connection to cancer and fibrosis. Researchers have identified its involvement in the suppression of metastasis in several cancers including breast cancer by interacting with proteins like E-cadherin which maintain cell adhesion. In fibrosis alterations in KLF17 expression can disrupt normal tissue repair processes exacerbating the progression of fibrotic diseases by interacting with mediators involved in the TGF-β signaling pathway.

Product promise

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1 product image

  • Western blot - Anti-KLF17 antibody [RP23040191] (ab308133), expandable thumbnail

    Western blot - Anti-KLF17 antibody [RP23040191] (ab308133)

    Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1) and Tissue extracts of Mouse Testis (Lane 2) and Rat Testis (Lane 3). The blots were probed with Anti-KLF17 Recombinant Rabbit Polyclonal
    Antibody (ab308133, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1:2500 dilution). A 55 kDa band corresponding to KLF17 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL)

    Lane 1: Nuclear enriched extracts of RAW 264.7 at 30 µg

    Lane 2: Mouse Testis tissue lysate at 30 µg

    Lane 3: Rat Testis tissue lysate at 30 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody HRP conjugate at 1/2500 dilution

    Developed using the ECL technique.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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