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AB308133

Anti-KLF17 antibody [RP23040191]

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Rabbit Recombinant Multiclonal KLF17 antibody. Suitable for WB and reacts with Mouse, Rat samples. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLF17.

View Alternative Names

ZNF393, KLF17, Krueppel-like factor 17, Zinc finger protein 393

1 Images
Western blot - Anti-KLF17 antibody [RP23040191] (AB308133)
  • WB

Supplier Data

Western blot - Anti-KLF17 antibody [RP23040191] (AB308133)

Western blot analysis was performed on Nuclear enriched extracts (30 µg lysate) of RAW 264.7 (Lane 1) and Tissue extracts of Mouse Testis (Lane 2) and Rat Testis (Lane 3). The blots were probed with Anti-KLF17 Recombinant Rabbit Polyclonal Antibody (ab308133, 1-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (0.4 µg/mL, 1 : 2500 dilution). A 55 kDa band corresponding to KLF17 was observed across the cell lines and tissues tested. Known quantity of protein samples were electrophoresed using a 4-12% Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed (ECL)

Lane 1:

Nuclear enriched extracts of RAW 264.7 at 30 µg

Lane 2:

Mouse Testis tissue lysate at 30 µg

Lane 3:

Rat Testis tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody HRP conjugate at 1/2500 dilution

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040191

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KLF17.

Q5JT82

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Reactivity data

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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.4 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF17 also known as Krüppel-like factor 17 is a zinc-finger transcription factor with a mass of approximately 35 kDa. This protein plays a role in DNA-binding and regulation of transcription. It regulates the expression of target genes by binding to GC-rich regions. Researchers have observed KLF17 expression in various tissues with notable levels in the reproductive organs such as the ovary and testis as well as in embryonic tissues which indicates its involvement in reproductive biology and development.
Biological function summary

KLF17 influences cellular processes by modulating the expression of genes involved in cell differentiation and proliferation. It functions primarily as a transcriptional repressor acting independently and not as part of a larger multiprotein complex. Its regulatory role affects key cellular functions contributing to the control of epithelial-mesenchymal transition (EMT) which is critical during embryogenesis and cancer metastasis.

Pathways

KLF17 plays a significant role in the TGF-β signaling pathway which is important in numerous cellular functions such as growth and differentiation. KLF17 interacts with other transcription factors like SNAIL and TWIST influencing pathways that control EMT and metastasis. This regulation within TGF-β signaling highlights KLF17's role in maintaining cellular architecture and regulating processes critical to tissue development and disease progression.

KLF17 shows a connection to cancer and fibrosis. Researchers have identified its involvement in the suppression of metastasis in several cancers including breast cancer by interacting with proteins like E-cadherin which maintain cell adhesion. In fibrosis alterations in KLF17 expression can disrupt normal tissue repair processes exacerbating the progression of fibrotic diseases by interacting with mediators involved in the TGF-β signaling pathway.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development (By similarity).
See full target information KLF17
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Product promise

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