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AB327155

Anti-KLF2 Antibody [CL14502]

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Mouse Monoclonal KLF2 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human KLF2.

View Alternative Names

LKLF, KLF2, Krueppel-like factor 2, Lung krueppel-like factor

7 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunohistochemical staining of human small intestine using ab327155 at 1 : 500–1 : 1000 dilution, showing moderate nuclear positivity in glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunohistochemical staining of human testis using ab327155 at 1 : 500–1 : 1000 dilution, showing moderate to strong nuclear positivity in cells in seminiferous ducts.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunohistochemical staining of human lymph node using ab327155 at 1 : 500–1 : 1000 dilution, showing moderate nuclear positivity in germinal and non-germinal center cells.

Immunocytochemistry/ Immunofluorescence - Anti-KLF2 Antibody [CL14502] (AB327155)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunofluorescence staining in U-251MG cell line using ab327155 at 2–10 µg/ml, showing specific staining of nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue respectively (where available).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunohistochemical staining of human heart muscle using ab327155 at 1 : 500–1 : 1000 dilution, showing moderate to strong nuclear positivity in cardiomyocytes.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunohistochemistry analysis in human lymph node and cerebral cortex tissues using ab327155 at 1 : 500–1 : 1000 dilution. Corresponding KLF2 RNA-seq data are presented for the same tissues.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF2 Antibody [CL14502] (AB327155)

Immunohistochemical staining of human cerebral cortex using ab327155 at 1 : 500–1 : 1000 dilution, showing no positivity in neurons as expected.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

CL14502

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Synthetic Peptide within Human KLF2.

Q9Y5W3

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2-10 µg/mL", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Transcription factor that binds to the CACCC box in the promoter of target genes such as HBB/beta globin or NOV and activates their transcription (PubMed : 21063504). Might be involved in transcriptional regulation by modulating the binding of the RARA nuclear receptor to RARE DNA elements (PubMed : 28167758).
See full target information KLF2

Product promise

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