Rabbit Polyclonal KLF4 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human KLF4.
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- Pharmacology 109:34-422023Impairment of Autophagy Mediates the Uric-Acid-Induced Phenotypic Transformation of Vascular Smooth Muscle Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYan Lu,Hanlin Zhang,Min Han,Ping Wang,Liping MengPubMed 38011839
- Nature communications 14:53602023FAM3A reshapes VSMC fate specification in abdominal aortic aneurysm by regulating KLF4 ubiquitination.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuxiang Lei,Haoxuan Kan,Xiangyu Xian,Wenlin Chen,Wenxuan Xiang,Xiaohong Song,Jianqiang Wu,Dan Yang,Yuehong ZhengPubMed 37660071
- iScience 25:1051942022Oncofetal protein IGF2BP1 regulates IQGAP3 expression to maintain stem cell potential in cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKhine Myint,Linda Shyue Huey Chuang,Yu Xuan Teh,Nur Astiana Mawan,Edward Jizhong Shi,Michelle Meng Huang Mok,Napat Nuttonmanit,Junichi Matsuo,Ying Li,Henry Yang,Atsushi Okabe,Atsushi Kaneda,Motomi Osato,Jimmy Bok-Yan So,Wei Peng Yong,Patrick Tan,Khay Guan Yeoh,Yoshiaki ItoPubMed 36217548
- Bioactive materials 21:69-852022An esterase-responsive ibuprofen nano-micelle pre-modified embryo derived nucleus pulposus progenitor cells promote the regeneration of intervertebral disc degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKai-Shun Xia,Dong-Dong Li,Cheng-Gui Wang,Li-Wei Ying,Jing-Kai Wang,Biao Yang,Jia-Wei Shu,Xian-Peng Huang,Yu-Ang Zhang,Chao Yu,Xiao-Peng Zhou,Fang-Cai Li,Nigel K H Slater,Jian-Bin Tang,Qi-Xin Chen,Cheng-Zhen LiangPubMed 36017070
- Cell reports 40:1112402022Krüppel-like factor 5 rewires NANOG regulatory network to activate human naive pluripotency specific LTR7Ys and promote naive pluripotency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhipeng Ai,Xinyu Xiang,Yangquan Xiang,Iwona Szczerbinska,Yuli Qian,Xiao Xu,Chenyang Ma,Yaqi Su,Bing Gao,Hao Shen,Muhammad Nadzim Bin Ramli,Di Chen,Yue Liu,Jia-Jie Hao,Huck Hui Ng,Dan Zhang,Yun-Shen Chan,Wanlu Liu,Hongqing LiangPubMed 36001968
- Cell death discovery 8:1662022DUB3/KLF4 combats tumor growth and chemoresistance in hepatocellular carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuebing Jia,Lei Li,Fan Wang,Ying Xue,Tongde Wu,Qianqian Jia,Yunhui Li,Chenming Wu,Yuping Chen,Jinhuan Wu,Yang Su,Xinshu Wang,Tao Zhuang,Xiao Dong,Jing Ling,Jian Yuan,Qi LiPubMed 35383144
- Frontiers in physiology 12:7602232022circKLF4 Upregulates and to Promote Odontoblastic Differentiation of Mouse Dental Papilla Cells Sponging miRNA-1895 and miRNA-5046.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYue Zhang,Hao Zhang,Guohua Yuan,Guobin YangPubMed 35222058
- Cells 10:2021Death-Associated Protein Kinase 1 Inhibits Progression of Thyroid Cancer by Regulating Stem Cell Markers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMi-Hyeon You,Woo Kyung Lee,Meihua Jin,Dong Eun Song,Sheue-Yann Cheng,Tae Yong Kim,Won Bae Kim,Min Ji Jeon,Won Gu KimPubMed 34831219
- PloS one 16:e02579772021Transcription factors Krüppel-like factor 4 and paired box 5 regulate the expression of the Grainyhead-like genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGrzegorz Kotarba,Agnieszka Taracha-Wisniewska,Michal Miller,Michal Dabrowski,Tomasz WilanowskiPubMed 34570823
- Frontiers in cell and developmental biology 9:6587202021Sevoflurane Inhibits Traumatic Brain Injury-Induced Neuron Apoptosis EZH2-Downregulated KLF4/p38 Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhongyu Wang,Juan Li,Anqi Wang,Zhaoyang Wang,Junmin Wang,Jingjing Yuan,Xin Wei,Fei Xing,Wei Zhang,Na XingPubMed 34422795
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KLF4. The exact immunogen used to generate this antibody is proprietary information. Database link O43474
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Expected | Tested |
| Rat | Expected | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
Species Mouse | Dilution info 2 µg/mL | Notes - |
Species Rat | Dilution info 2 µg/mL | Notes - |
Associated Products
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Target data
Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Alternative names
EZF, GKLF, KLF4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor
Recommended products
Rabbit Polyclonal KLF4 antibody. Suitable for WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 43 publications. Immunogen corresponding to Synthetic Peptide within Human KLF4.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KLF4. The exact immunogen used to generate this antibody is proprietary information. Database link O43474
- Purification technique
- Affinity purification Immunogen
- Specificity
ab106629 will detect all three known isoforms. It does not cross-react with other KLF members.
- Concentration
- Purification notes
ab106629 is purified by affinity chromatography via a peptide column.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
Notes
For information on sample preparation for western blot, please refer here.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.
- Biological function summary
KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.
- Pathways
The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/β-catenin and Notch pathways. Within the Wnt/β-catenin pathway KLF4 may cooperate with β-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.
- Associated diseases and disorders
Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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4 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody (ab106629)
Immunohistochemical analysis of paraffin-embedded human colon tissue labeling KLF4 with ab106629 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody (ab106629)
Immunohistochemical analysis of paraffin-embedded rat colon tissue labeling KLF4 with ab106629 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody (ab106629)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue labeling KLF4 with ab106629 at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH 6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Western blot - Anti-KLF4 antibody (ab106629)
1 h incubation at RT in 5% NFDM/TBST. ab106629 detected the expression of KLF4 in MCF-7 cells, but not in Jurkat cells, which matches the RNA expression pattern in these two cell lines based on Human Protein Atlas data.
All lanes: Western blot - Anti-KLF4 antibody (ab106629) at 1 µg/mL
Lane 1: MCF-7 cell lysate at 15 µg
Lane 2: Jurkat cell lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
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