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AB216875

Anti-KLF4 antibody

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(4 Publications)

Rabbit Polyclonal KLF4 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 4 publications.

View Alternative Names

EZF, GKLF, KLF4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (AB216875)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody (AB216875)

Immunofluorescent analysis of 0.5% PFA-fixed (A and C) and 0.5% methanol-fixed (B and D) PC-3 (human prostate adenocarcinoma cell line) cells labeling KLF4 with ab216875 at 10 μg/ml (green).

The nuclear counter stain is DAPI (blue).

Secondary antibody only control : C and D.

Western blot - Anti-KLF4 antibody (AB216875)
  • WB

Supplier Data

Western blot - Anti-KLF4 antibody (AB216875)

Blocking buffer : Blotto 5%.

Dilution buffer : 1X PBS, 0.01% azide.

All lanes:

Western blot - Anti-KLF4 antibody (ab216875) at 1/1000 dilution

Lanes 1 and 13:

MW ladder (opal pre-stained)

Lane 2:

U-87 whole cell lysate

Lane 3:

C2C12 whole cell lysate

Lane 4:

NIH/3T3 whole cell lysate

Lane 5:

THP-1 whole cell lysate

Lane 6:

J774A.1 whole cell lysate

Lane 7:

PC-3 whole cell lysate

Lane 8:

HT-29 whole cell lysate

Lane 9:

Mouse colon lysate

Lane 10:

A549 whole cell lysate

Lane 11:

MOLT-4 whole cell lysate

Lane 12:

HeLa whole cell lysate

Secondary

All lanes:

Rabbit secondary HRP antibody at 1/70000 dilution

Predicted band size: 54 kDa

Observed band size: 55 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.
Biological function summary

KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.

Pathways

The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/β-catenin and Notch pathways. Within the Wnt/β-catenin pathway KLF4 may cooperate with β-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.

Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
See full target information KLF4
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

PLoS biology 23:e3002654 PubMed40720543

2025

Sox5 controls the establishment of quiescence in neural stem cells during postnatal development.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Medina-Menéndez,Paula Tirado-Melendro,Lingling Li,Pilar Rodríguez-Martín,Elena Melgarejo-de la Peña,Mario Díaz-García,María Valdés-Bescós,Rafael López-Sansegundo,Aixa V Morales

Stem cell investigation 10:10 PubMed37155477

2023

Transcriptomic analysis of feeder-free culture system for maintaining naïve-state pluripotency in human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Isono,Tomoyuki Kawasaki,Justin K Ichida,Kazunori Nagasaka,Osamu Hiraike,Akihiro Umezawa,Hidenori Akutsu

Biological research 56:17 PubMed37016436

2023

Spontaneous spheroids from alveolar bone-derived mesenchymal stromal cells maintain pluripotency of stem cells by regulating hypoxia-inducible factors.

Applications

Unspecified application

Species

Unspecified reactive species

Ni Li,Xiaofeng Dai,Fei Yang,Yang Sun,Xingwen Wu,Qianrong Zhou,Kai Chen,Jian Sun,Wei Bi,Le Shi,Youcheng Yu

Regenerative therapy 15:161-168 PubMed33426214

2020

The combination of dibenzazepine and a DOT1L inhibitor enables a stable maintenance of human naïve-state pluripotency in non-hypoxic conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Wataru Isono,Tomoyuki Kawasaki,Justin K Ichida,Takuya Ayabe,Osamu Hiraike,Akihiro Umezawa,Hidenori Akutsu
View all publications
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