Rabbit Recombinant Monoclonal KLF4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, ChIP-seq and reacts with Human samples.
Images
![Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunoprecipitation-img20591.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235)")
![Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunocytochemistry-immunofluorescence-img14834.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115360.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235)")
![Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--flow-cytometry-intracellular-img140513.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88900.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (AB222235)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | ChIP-seq | |
|---|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Tested | Tested | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ChIP-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Alternative names
EZF, GKLF, KLF4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor
Recommended products
Rabbit Recombinant Monoclonal KLF4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P, ChIP-seq and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19590
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab222235 is the carrier-free version of Anti-KLF4 antibody [EPR19590] ab215036.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.
- Biological function summary
KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.
- Pathways
The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/β-catenin and Notch pathways. Within the Wnt/β-catenin pathway KLF4 may cooperate with β-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.
- Associated diseases and disorders
Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
9 product images
![Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunoprecipitation-img20591.jpg "Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
Immunoprecipitation - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
Anti-KLF4 antibody [EPR19590] ab215036 (purified) at 1/30 dilution immunoprecipitating KLF4 in NCCIT whole cell lysate.
Lane 1 (input): NCCIT (Human pluripotent embryonic carcinoma epithelial cell) whole cell lysate 10 μg
Lane 2 (+): Anti-KLF4 antibody [EPR19590] ab215036 & NCCIT whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-KLF4 antibody [EPR19590] ab215036 in NCCIT whole cell lysate
For western blotting, Anti-KLF4 antibody [EPR19590] ab215036 at 1/500 dilution (0.117 μg/ml) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution were used.
Blocking and diluting buffer: 5% NFDM /TBST .
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR19590] ab215036)All lanes: Immunoprecipitation - Anti-KLF4 antibody [EPR19590] (Anti-KLF4 antibody [EPR19590] ab215036)
Predicted band size: 15 kDa, 54 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunocytochemistry-immunofluorescence-img14834.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling KLF4 with Anti-KLF4 antibody [EPR19590] ab215036 at 1/100 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on NCCIT cell line.
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 (Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594)) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR19590] ab215036).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115360.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
Immunohistochemical analysis of paraffin-embedded human stomach tissue labeling KLF4 with Anti-KLF4 antibody [EPR19590] ab215036 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Nuclear staining on human stomach is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR19590] ab215036).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--flow-cytometry-intracellular-img140513.jpg "Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
Flow Cytometry (Intracellular) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed NCCIT (Human pluripotent embryonic carcinoma cell line) cells labeling KLF4 with Anti-KLF4 antibody [EPR19590] ab215036 at 1/50 dilution (red) compared with a rabbit monoclonal IgG isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR19590] ab215036).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88900.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling KLF4 with Anti-KLF4 antibody [EPR19590] ab215036 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Nuclear staining on tumor cells of human gastric cancer is observed [PMID: 17614846].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR19590] ab215036).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74723.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling KLF4 with Anti-KLF4 antibody [EPR19590] ab215036 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
Nuclear staining on tumor cells of human seminoma is observed [PMID: 17614846].
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR19590] ab215036).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--chip-sequencing-img439388.jpg "ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
This data was developed using the same antibody clone in a different buffer formulation (Anti-KLF4 antibody [EPR19590] ab215036).
Chromatin was prepared from NCCIT cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of Anti-KLF4 antibody [EPR19590] ab215036 [EPR19590]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.
![ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--chip-sequencing-img439386.jpg "ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
This data was developed using the same antibody clone in a different buffer formulation (Anti-KLF4 antibody [EPR19590] ab215036).
Chromatin was prepared from NCCIT cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of Anti-KLF4 antibody [EPR19590] ab215036 [EPR19590]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.
![ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--chip-sequencing-img439387.jpg "ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)
This data was developed using the same antibody clone in a different buffer formulation (Anti-KLF4 antibody [EPR19590] ab215036).
Chromatin was prepared from NCCIT cells. Cells were fixed with 1% formaldehyde for 10 minutes. ChIP was performed with 107 cells and 8 µg of Anti-KLF4 antibody [EPR19590] ab215036 [EPR19590]. ChIP DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 30 million reads. The input control is also shown.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img74723.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
![ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--chip-sequencing-img439388.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
![ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--chip-sequencing-img439386.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
![ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr19590-bsa-and-azide-free-ab222235--chip-sequencing-img439387.jpg/_jcr_content/renditions/webp-475.webp "ChIP-sequencing - Anti-KLF4 antibody [EPR19590] - BSA and Azide free (ab222235)")
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