Rabbit Recombinant Monoclonal KLF4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC - Wmt, IHC-P and reacts with Mouse, Rat samples.
Images
![IHC - Wholemount - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--ihc-wholemount-img68588.jpg/_jcr_content/renditions/webp-475.webp "IHC - Wholemount - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335)")
![Immunoprecipitation - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunoprecipitation-img3891.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21716.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112827.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335)")
![Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunocytochemistry-immunofluorescence-img129758.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (AB255335)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | IHC - Wmt | IHC-P | |
|---|---|---|---|---|---|
| Mouse | Tested | Expected | Tested | Tested | Expected |
| Rat | Expected | Expected | Expected | Expected | Tested |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC - Wmt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
2 products for Alternative Version
Target data
Function
Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Binds to the promoter region of its own gene and can activate its own transcription (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Regulates the expression of key transcription factors during embryonic development (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation (PubMed:10431239, PubMed:10556311, PubMed:15358627, PubMed:16954384, PubMed:17060454, PubMed:19816951, PubMed:20071344, PubMed:29593216). Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription (By similarity).
Alternative names
Ezf, Gklf, Zie, Klf4, Krueppel-like factor 4, Epithelial zinc finger protein EZF, Gut-enriched krueppel-like factor
Recommended products
Rabbit Recombinant Monoclonal KLF4 antibody. Carrier free. Suitable for IP, WB, ICC/IF, IHC - Wmt, IHC-P and reacts with Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20753-25
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab255335 is the carrier-free version of Anti-KLF4 antibody [EPR20753-25] ab214666.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLF4 also known as Kruppel-like factor 4 acts as a transcription factor involved in the regulation of gene expression. It has a molecular weight of approximately 55-60 kDa. Researchers often find KLF4 expressed in epithelial tissues such as the skin and gastrointestinal tract. It regulates cellular processes like differentiation proliferation and apoptosis playing a role in maintaining homeostasis and cell identity.
- Biological function summary
KLF4 impacts cell differentiation and proliferation through its role as a transcription factor. It functions within several cellular complexes and interacts with other transcription factors to modulate gene expression. KLF4 regulates stem cell maintenance by influencing gene networks involved in self-renewal and differentiation contributing particularly to pluripotency in embryonic stem cells.
- Pathways
The transcriptional activity of KLF4 integrates into key signaling pathways like the Wnt/β-catenin and Notch pathways. Within the Wnt/β-catenin pathway KLF4 may cooperate with β-catenin and TCF/LEF transcription factors to modulate gene targets. Similarly in the Notch signaling pathway KLF4 interacts with pathway components to influence cell fate decisions. These interactions highlight KLF4's central role in orchestrating complex signaling networks.
- Associated diseases and disorders
Mutations or dysregulated expression of KLF4 associates with various conditions including colorectal cancer and skin disorders like atopic dermatitis. In colorectal cancer KLF4 often interacts with other oncogenes and tumor suppressor proteins such as p53 to influence tumorigenesis. In skin disorders altered KLF4 expression can impact skin barrier function and inflammatory responses connecting it with proteins involved in immune response regulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![IHC - Wholemount - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--ihc-wholemount-img68588.jpg "IHC - Wholemount - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)")
Image courtesy of Vidur Garg from the Sloan-Kettering InstituteIHC - Wholemount - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)
This data was developed using Anti-KLF4 antibody [EPR20753-25] ab214666, the same antibody clone in a different buffer formulation.
Several embryos at the mid-blastocyst stage (~E3.75) were tested. The embryos were fixed for 10 mins at room temperature in 4% PFA. Fixed embryos were washed for 5 mins in PBS + 0.1% Triton X-100 (PBX) at room temperature. The embryos were then permeabilized in PBS + 0.5% Triton X-100 + 100mM Glycine for 5 mins at room temperature and washed again in PBX for 5 mins at room temperature. Blocking was carried out for 1 hour at room temperature in PBS + 2% horse serum and then the embryos were incubated overnight in Anti-KLF4 antibody [EPR20753-25] ab214666 (1/1000) diluted in blocking solution. The next day embryos were washed three times for 5 mins each in PBX, before blocking again for 1 hour at room temperature. Then incubated in Donkey anti rabbit Alexa Fluor® 488 secondary antibodies (1/500) diluted in blocking solution for 1 hour at 4°C. This was followed by 2 washes of 5 mins each in PBX. The embryos were then incubated in PBS + 5μg/ml DAPI for 10 min, or until mounting for imaging. Images were acquired on a laser-scanning confocal microscope system. Displayed image shows maximum intensity projection of 5 optical sections acquired at 1μm increments. Scale bar: 20 μm
![Immunoprecipitation - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunoprecipitation-img3891.jpg "Immunoprecipitation - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)")
Immunoprecipitation - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)
KLF4 was immunoprecipitated from 0.35 mg F9 (Mouse embryonal carcinoma epithelial cell) whole cell lysate using Anti-KLF4 antibody [EPR20753-25] ab214666 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-KLF4 antibody [EPR20753-25] ab214666 at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used as the secondary at 1/5000 dilution.
Lane 1: F9 whole cell lysate 10 µg (input).
Lane 2: Anti-KLF4 antibody [EPR20753-25] ab214666 IP in F9 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-KLF4 antibody [EPR20753-25] ab214666 in F9 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
Fresh lysate was required and IP test was conducted immediately. Incubation time was shortened from overnight to 2h.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR20753-25] ab214666).
All lanes: Immunoprecipitation - Anti-KLF4 antibody [EPR20753-25] (Anti-KLF4 antibody [EPR20753-25] ab214666)
Predicted band size: 52 kDa, 54 kDa
Observed band size: 52 kDa, 59 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img21716.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling KLF4 with Anti-KLF4 antibody [EPR20753-25] ab214666 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on epithelial cells of rat colon (PMID: 21070761, PMID: 19109406) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR20753-25] ab214666).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112827.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling KLF4 with Anti-KLF4 antibody [EPR20753-25] ab214666 at 1/1000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) secondary antibody. Nuclear staining on epithelial cells of mouse colon (PMID: 21070761, PMID: 19109406) is observed. Counterstained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR20753-25] ab214666).
Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
![Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335), expandable thumbnail](https://content.abcam.com/products/images/klf4-antibody-epr20753-25-bsa-and-azide-free-ab255335--immunocytochemistry-immunofluorescence-img129758.jpg "Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)")
Immunocytochemistry/ Immunofluorescence - Anti-KLF4 antibody [EPR20753-25] - BSA and Azide free (ab255335)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma epithelial cell) cells labeling KLF4 with Anti-KLF4 antibody [EPR20753-25] ab214666 at 1/100 dilution, followed by a AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 dilution (Green). Confocal image showing nuclear staining in F9 cell line is observed. The nuclear counterstain was DAPI (Blue). Tubulin was counterstained using an Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594, Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a AlexaFluor®488 Goat anti-Rabbit secondary (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-KLF4 antibody [EPR20753-25] ab214666).
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