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AB277773

Anti-KLF5 antibody [23HCLC]

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(3 Publications)

Rabbit Recombinant Multiclonal KLF5 antibody. Suitable for ICC, ChIP and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KLF5 aa 350 to C-terminus.

View Alternative Names

BTEB2, CKLF, IKLF, KLF5, Krueppel-like factor 5, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, BTE-binding protein 2

2 Images
Immunocytochemistry - Anti-KLF5 antibody [23HCLC] (AB277773)
  • ICC

Supplier Data

Immunocytochemistry - Anti-KLF5 antibody [23HCLC] (AB277773)

For immunofluorescence analysis, A549 cells were fixed and permeabilized for detection of endogenous KLF5 using Anti- KLF5 Recombinant Rabbit Polyclonal Antibody (ab277773, 2 μg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (1/2000). Panel a) shows representative cells that were stained for detection and localization of KLF5 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (1/300). Panel d) is a composite image of Panels a, b and c demonstrating predominant nuclear localization of KLF5. Panel e) represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ChIP - Anti-KLF5 antibody [23HCLC] (AB277773)
  • ChIP

Supplier Data

ChIP - Anti-KLF5 antibody [23HCLC] (AB277773)

Enrichment of endogenous KLF5 protein at specific gene loci using Anti- KLF5 Recombinant Rabbit Polyclonal Antibody : Chromatin Immunoprecipitation (ChIP) was performed using Anti-KLF5 Recombinant Rabbit Polyclonal Antibody (ab277773, 5 μg) on sheared chromatin from 2 million A549 cells using the "MAGnify ChIP system" kit. Normal Rabbit IgG (1 μg) was used as a negative IP control. The purified DNA was analyzed by 7500 Fast qPCR system with optimized PCR primer pairs for the promoters of the active SOX4, KLF4 region used as positive control target gene, and the region of the inactive KLF4 UPSTRAEM, SAT2 satellite repeat, used as negative control target gene. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

23HCLC

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, ChIP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Synthetic Peptide within Human KLF5 aa 1-50. Database link Q13887

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "2.5-5 µg/cells for 6 µg/cells", "ChIP-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF5 (Kruppel-like factor 5) also referred to as BTEB2 is a transcription factor with a molecular mass of approximately 54 kDa. It belongs to the KLF family characterized by its three C2H2-type zinc finger domains which bind to specific DNA sequences regulating gene expression. KLF5 displays ubiquitous expression but is highly concentrated in epithelial tissues specifically in the gastrointestinal tract and skin. This factor plays an essential role in the regulation of cell proliferation and differentiation.
Biological function summary

Kruppel-like factor 5 modulates gene transcription by binding to GC-rich elements in promoter regions influencing various cellular processes. KLF5 can interact with other transcription factors and co-regulators forming complexes important for its function. It significantly impacts epithelial cell dynamics affecting cell transformation differentiation and migration. In particular it plays a pivotal role in maintaining the proliferative capacity of epithelial cells and supporting wound healing processes.

Pathways

Kruppel-like factor 5 holds a notable position in the TGF-beta and Wnt signaling pathways which govern cellular growth control and development. In the TGF-beta pathway KLF5 can antagonize SMAD-mediated transcription suppression promoting cell proliferation particularly in epithelial stem cells. In the Wnt pathway it influences beta-catenin activity essential for epithelial cell renewal and maintenance. Through these pathways KLF5 interacts with proteins such as SMAD3 and beta-catenin coordinating cell turnover and tissue homeostasis.

KLF5 impacts cancer and cardiovascular diseases. It plays a complex role in oncogenesis where its overexpression is associated with colorectal breast and prostate cancers due to its ability to enhance proliferation. KLF5's interaction with proteins like p53 influences tumorigenic potentials. In cardiovascular disorders KLF5 contributes to vascular pathology affecting smooth muscle cell proliferation and atherosclerosis. Through its regulation of cyclin D1 it integrates into pathways leading to abnormal vascular smooth muscle growth linking it further to cardiovascular complications.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
See full target information KLF5
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of cell communication and signaling 19:e12060 PubMed39720765

2024

M2-polarized tumor-associated macrophage-secreted exosomal lncRNA NEAT1 upregulates galectin-3 by recruiting KLF5 and promotes HCC immune escape.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Yuan,Qigang Sun,Xiaodan Zhu,Bo Li,Yongping Zou,Zhehao Liu

Medical oncology (Northwood, London, England) 41:17 PubMed38087142

2023

KLF5 promotes esophageal squamous cell cancer through the transcriptional activation of FGFBP1.

Applications

Unspecified application

Species

Unspecified reactive species

Fengyun Wang,Ming Luo,Yufeng Cheng

International journal of molecular medicine 48: PubMed34165154

2021

KLF5/LINC00346/miR‑148a‑3p axis regulates inflammation and endothelial cell injury in atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Fangfang Wang,Jiyong Ge,Shenglan Huang,Changle Zhou,Zikai Sun,Ying Song,Yajing Xu,Yuan Ji
View all publications
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Product promise

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