Anti-KLF5 antibody

• WB

Lab

Western blot - Anti-KLF5 antibody (AB137676)

Western blot : Anti-KLF5 antibody (ab137676) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137676 was shown to bind specifically to KLF5. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in KLF5 knockout cell line. To generate this image, wild-type and KLF5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

KLF5 knockout A549 cell lysate at 20 µg

Lane 3:

HAP1 cell lysate at 20 µg

Lane 4:

OVCAR-3 cell lysate at 20 µg

Lane 5:

PANC-1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 55 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (AB137676)

ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (AB137676)

Immunofluorescence analysis of 4% paraformaldehyde fixed at RT for 15 mins HeLa cells labelling KLF5 protein at nucleus using ab137676 at a 1/500 dilution (Green). Alpha tubulin, a cytoskeleton marker stained by anti-alpha tubulin antibody at a 1/1000 dilution (Red). The nuclear counterstain is DAPI (Blue).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (AB137676)

Paraffin-embedded mouse intestine tissue stained for KLF5 protein at nucleus with ab137676 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (AB137676)

Paraffin-embedded mouse testis tissue stained for KLF5 protein at nucleus with ab137676 at a 1/500 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• WB

Supplier Data

Western blot - Anti-KLF5 antibody (AB137676)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

KLF5 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-KLF5 antibody (AB137676)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

Lane 1:

Wild-type (WT) HeLa cell extracts at 30 µg

Lane 2:

KLF5 knockout (KO) HeLa cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-KLF5 antibody (AB137676)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

Lane 1:

HeLa Whole cell extract at 30 µg

Lane 2:

HeLa nuclear extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false

• WB

Supplier Data

Western blot - Anti-KLF5 antibody (AB137676)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

Lane 1:

Colo 205 whole cell extracts at 30 µg

Lane 2:

MCF-7 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false

• WB

Lab

Western blot - Anti-KLF5 antibody (AB137676)

Western blot : Anti-KLF5 antibody ab137676 staining at 1/2000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in KLF5 knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/2000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human KLF5 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-klf5-knockout-hct116-cell-line-ab301030'>ab301030</a>) at 20 µg

Lane 3:

Western blot - Human wild-type A549 cell line (ab288558) at 20 µg

Lane 4:

Western blot - Human KLF5 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-klf5-knockout-a549-cell-line-ab301031'>ab301031</a>) at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 51 kDa,55 kDa

Observed band size: 55 kDa,37 kDa

false

• WB

Supplier Data

Western blot - Anti-KLF5 antibody (AB137676)

Samples were separated by 10% SDS-PAGE.

All lanes:

Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

Lane 1:

A549 whole cell extracts at 30 µg

Lane 2:

H1299 whole cell extracts at 30 µg

Lane 3:

HCT116 whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 51 kDa

false