Rabbit Polyclonal KLF5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 45 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Krueppel-like factor 5 aa 50-350.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
WB | IHC-P | ICC/IF | |
---|---|---|---|
Human | Tested | Tested | Tested |
Mouse | Expected | Expected | Tested |
Cow | Predicted | Predicted | Predicted |
Pig | Predicted | Predicted | Predicted |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
BTEB2, CKLF, IKLF, IKLF, CKLF, BTEB2, KLF5, Krueppel-like factor 5, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, BTE-binding protein 2
Rabbit Polyclonal KLF5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 45 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Krueppel-like factor 5 aa 50-350.
IgG
Rabbit
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Liquid
Polyclonal
Affinity purification Immunogen
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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This supplementary information is collated from multiple sources and compiled automatically.
KLF5 (Kruppel-like factor 5) also referred to as BTEB2 is a transcription factor with a molecular mass of approximately 54 kDa. It belongs to the KLF family characterized by its three C2H2-type zinc finger domains which bind to specific DNA sequences regulating gene expression. KLF5 displays ubiquitous expression but is highly concentrated in epithelial tissues specifically in the gastrointestinal tract and skin. This factor plays an essential role in the regulation of cell proliferation and differentiation.
Kruppel-like factor 5 modulates gene transcription by binding to GC-rich elements in promoter regions influencing various cellular processes. KLF5 can interact with other transcription factors and co-regulators forming complexes important for its function. It significantly impacts epithelial cell dynamics affecting cell transformation differentiation and migration. In particular it plays a pivotal role in maintaining the proliferative capacity of epithelial cells and supporting wound healing processes.
Kruppel-like factor 5 holds a notable position in the TGF-beta and Wnt signaling pathways which govern cellular growth control and development. In the TGF-beta pathway KLF5 can antagonize SMAD-mediated transcription suppression promoting cell proliferation particularly in epithelial stem cells. In the Wnt pathway it influences beta-catenin activity essential for epithelial cell renewal and maintenance. Through these pathways KLF5 interacts with proteins such as SMAD3 and beta-catenin coordinating cell turnover and tissue homeostasis.
KLF5 impacts cancer and cardiovascular diseases. It plays a complex role in oncogenesis where its overexpression is associated with colorectal breast and prostate cancers due to its ability to enhance proliferation. KLF5's interaction with proteins like p53 influences tumorigenic potentials. In cardiovascular disorders KLF5 contributes to vascular pathology affecting smooth muscle cell proliferation and atherosclerosis. Through its regulation of cyclin D1 it integrates into pathways leading to abnormal vascular smooth muscle growth linking it further to cardiovascular complications.
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All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/5000 dilution
Lane 1: Non-transfected 293T whole cell extract at 30 µg
Lane 2: Transfected 293T whole cell extract at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 137 kDa, 51 kDa
Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution
Lane 1: A549 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HCT116 whole cell lysate at 30 µg
All lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF.
ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500
10% SDS PAGE
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 47 kDa, 51 kDa, 53 kDa
Western blot: Anti-KLF5 antibody (ab137676) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137676 was shown to bind specifically to KLF5. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in KLF5 knockout cell line. To generate this image, wild-type and KLF5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: KLF5 knockout A549 cell lysate at 20 µg
Lane 3: HAP1 cell lysate at 20 µg
Lane 4: OVCAR-3 cell lysate at 20 µg
Lane 5: PANC-1 cell lysate at 20 µg
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 55 kDa
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