Anti-KLF5 antibody (ab137676) is a rabbit polyclonal antibody detecting KLF5 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.
- Over 40 publications
Images
Publications
Filter by
- Medical oncology (Northwood, London, England) 41:172023KLF5 promotes esophageal squamous cell cancer through the transcriptional activation of FGFBP1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFengyun Wang,Ming Luo,Yufeng ChengPubMed 38087142
- Cancers 15:2023Krüppel-like Factor 5 Plays an Important Role in the Pathogenesis of Chronic Pancreatitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaryam Alavi,Ana Mejia-Bautista,Meiyi Tang,Jela Bandovic,Avi Z Rosenberg,Agnieszka B BialkowskaPubMed 38001687
- Oncogenesis 12:352023MLK4 promotes glucose metabolism in lung adenocarcinoma through CREB-mediated activation of phosphoenolpyruvate carboxykinase and is regulated by KLF5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlvin Ho-Kwan Cheung,Kit-Yee Wong,Xiaoli Liu,Fenfen Ji,Chris Ho-Lam Hui,Yihan Zhang,Johnny Sheung-Him Kwan,Bonan Chen,Yujuan Dong,Raymond Wai-Ming Lung,Jun Yu,Kwok Wai Lo,Chi Chun Wong,Wei Kang,Ka-Fai ToPubMed 37407566
- Cell genomics 3:1002292023Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKyung Duk Koh,Luke R Bonser,Walter L Eckalbar,Ofer Yizhar-Barnea,Jiangshan Shen,Xiaoning Zeng,Kirsten L Hargett,Dingyuan I Sun,Lorna T Zlock,Walter E Finkbeiner,Nadav Ahituv,David J ErlePubMed 36777184
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e227332023Human umbilical cord mesenchymal stem cells-derived exosomal circDLGAP4 promotes angiogenesis after cerebral ischemia-reperfusion injury by regulating miR-320/KLF5 axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Feng,Wei He,Jian Xia,Qing Huang,Jie Yang,Wen-Ping Gu,Ning Zhang,Yun-Hai LiuPubMed 36723877
- Antioxidants (Basel, Switzerland) 11:2022Prevention of LPS-Induced Acute Kidney Injury in Mice by Bavachin and Its Potential Mechanisms.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKa-Yun Ban,Ga-Young Nam,Donghee Kim,Yoon Sin Oh,Hee-Sook JunPubMed 36358467
- Genes & development :2022KLF5 governs sphingolipid metabolism and barrier function of the skin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Lyu,Yinglu Guan,Lisa Deliu,Ericka Humphrey,Joanna K Frontera,Youn Joo Yang,Daniel Zamler,Kun Hee Kim,Vakul Mohanty,Kevin Jin,Vakul Mohanty,Virginia Liu,Jinzhuang Dou,Lucas J Veillon,Shwetha V Kumar,Philip L Lorenzi,Yang Chen,Kathleen M McAndrews,Sergei Grivennikov,Xingzhi Song,Jianhua Zhang,Yuanxin Xi,Jing Wang,Ken Chen,Priyadharsini Nagarajan,Yejing GePubMed 36008138
- Systems biology in reproductive medicine 68:370-3832022LINC00665 affects the malignant biological behavior of ovarian cancer via the miR-148b-3p/KLF5.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShenglan Wang,Chuanchuan Liu,Yongchuan Li,Jinwan Qiao,Xinling Chen,Jin Bao,Ran Li,Yanxia XingPubMed 36016468
- Oncology letters 24:2582022KLF5-induced miR-487a augments the progression of osteosarcoma cells by targeting NKX3-1 in vitro.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnyu Luo,Hanlin Liu,Chen HuangPubMed 35765275
- Science advances 8:eabk15382022ARAF suppresses ERBB3 expression and metastasis in a subset of lung cancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuliane Mooz,Kristina Riegel,Hari Ps,Anguraj Sadanandam,Federico Marini,Matthias Klein,Ulrike Werner,Wilfried Roth,Annett Wilken-Schmitz,Irmgard Tegeder,Krishnaraj RajalingamPubMed 35302851
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KLF5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13887
Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Expected | Expected | Tested |
| Cow | Predicted | Predicted | Predicted |
| Pig | Predicted | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Target data
Function
Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.
Alternative names
BTEB2, CKLF, IKLF, KLF5, Krueppel-like factor 5, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, BTE-binding protein 2
Recommended products
Anti-KLF5 antibody (ab137676) is a rabbit polyclonal antibody detecting KLF5 in Western Blot, IHC-P, ICC/IF. Suitable for Human, Mouse.
- Over 40 publications
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KLF5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13887
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Anti-KLF5 antibody (ab137676) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse samples.
What is the molecular weight of KLF5?
Anti-KLF5 (ab137676) specifically detects a band for KLF5 (UniProt: Q13887) at a molecular weight of 51kDa.
Trusted by the scientific community
Anti-KLF5 (ab137676) was first used in a scientific publication in 2012 and has been cited over 40 times in peer-reviewed journals.
Reviewed by scientists
Anti-KLF5 (ab137676) has over 5 independent reviews from customers.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLF5 (Kruppel-like factor 5) also referred to as BTEB2 is a transcription factor with a molecular mass of approximately 54 kDa. It belongs to the KLF family characterized by its three C2H2-type zinc finger domains which bind to specific DNA sequences regulating gene expression. KLF5 displays ubiquitous expression but is highly concentrated in epithelial tissues specifically in the gastrointestinal tract and skin. This factor plays an essential role in the regulation of cell proliferation and differentiation.
- Biological function summary
Kruppel-like factor 5 modulates gene transcription by binding to GC-rich elements in promoter regions influencing various cellular processes. KLF5 can interact with other transcription factors and co-regulators forming complexes important for its function. It significantly impacts epithelial cell dynamics affecting cell transformation differentiation and migration. In particular it plays a pivotal role in maintaining the proliferative capacity of epithelial cells and supporting wound healing processes.
- Pathways
Kruppel-like factor 5 holds a notable position in the TGF-beta and Wnt signaling pathways which govern cellular growth control and development. In the TGF-beta pathway KLF5 can antagonize SMAD-mediated transcription suppression promoting cell proliferation particularly in epithelial stem cells. In the Wnt pathway it influences beta-catenin activity essential for epithelial cell renewal and maintenance. Through these pathways KLF5 interacts with proteins such as SMAD3 and beta-catenin coordinating cell turnover and tissue homeostasis.
- Associated diseases and disorders
KLF5 impacts cancer and cardiovascular diseases. It plays a complex role in oncogenesis where its overexpression is associated with colorectal breast and prostate cancers due to its ability to enhance proliferation. KLF5's interaction with proteins like p53 influences tumorigenic potentials. In cardiovascular disorders KLF5 contributes to vascular pathology affecting smooth muscle cell proliferation and atherosclerosis. Through its regulation of cyclin D1 it integrates into pathways leading to abnormal vascular smooth muscle growth linking it further to cardiovascular complications.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
7 product images
Western blot - Anti-KLF5 antibody (ab137676)
Western blot: Anti-KLF5 antibody (ab137676) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137676 was shown to bind specifically to KLF5. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in KLF5 knockout cell line. To generate this image, wild-type and KLF5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: KLF5 knockout A549 cell lysate at 20 µg
Lane 3: HAP1 cell lysate at 20 µg
Lane 4: OVCAR-3 cell lysate at 20 µg
Lane 5: PANC-1 cell lysate at 20 µg
SecondaryAll lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 55 kDa
Western blot - Anti-KLF5 antibody (ab137676)
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/5000 dilution
Lane 1: Non-transfected 293T whole cell extract at 30 µg
Lane 2: Transfected 293T whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 137 kDa, 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)
Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.
Western blot - Anti-KLF5 antibody (ab137676)
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution
Lane 1: A549 whole cell lysate at 30 µg
Lane 2: H1299 whole cell lysate at 30 µg
Lane 3: HCT116 whole cell lysate at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG
Predicted band size: 51 kDa
Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)
HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab137676)
ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500
Western blot - Anti-KLF5 antibody (ab137676)
10% SDS PAGE
All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution
All lanes: H1299 whole cell lysate at 30 µg
Predicted band size: 47 kDa, 51 kDa, 53 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com