Skip to main content

Rabbit Polyclonal KLF5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 45 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Krueppel-like factor 5 aa 50-350.


Images

Western blot - Anti-KLF5 antibody (AB137676), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (AB137676), expandable thumbnail
  • Western blot - Anti-KLF5 antibody (AB137676), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (AB137676), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (AB137676), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human Krueppel-like factor 5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13887

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Expected
Tested
Cow
Predicted
Predicted
Predicted
Pig
Predicted
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/500.00000 - 1/3000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Expected
Expected

Species

Mouse

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Cow, Pig

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Species

Human

Dilution info

1/100.00000 - 1/1000.00000

Notes

-

Predicted
Predicted

Species

Cow, Pig

Dilution info

-

Notes

-

Target data

Function

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Alternative names

Recommended products

Rabbit Polyclonal KLF5 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse samples. Cited in 45 publications. Immunogen corresponding to Recombinant Fragment Protein within Human Krueppel-like factor 5 aa 50-350.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Recombinant Fragment Protein within Human Krueppel-like factor 5 aa 50-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13887
Purification technique

Affinity purification Immunogen

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KLF5 (Kruppel-like factor 5) also referred to as BTEB2 is a transcription factor with a molecular mass of approximately 54 kDa. It belongs to the KLF family characterized by its three C2H2-type zinc finger domains which bind to specific DNA sequences regulating gene expression. KLF5 displays ubiquitous expression but is highly concentrated in epithelial tissues specifically in the gastrointestinal tract and skin. This factor plays an essential role in the regulation of cell proliferation and differentiation.

Biological function summary

Kruppel-like factor 5 modulates gene transcription by binding to GC-rich elements in promoter regions influencing various cellular processes. KLF5 can interact with other transcription factors and co-regulators forming complexes important for its function. It significantly impacts epithelial cell dynamics affecting cell transformation differentiation and migration. In particular it plays a pivotal role in maintaining the proliferative capacity of epithelial cells and supporting wound healing processes.

Pathways

Kruppel-like factor 5 holds a notable position in the TGF-beta and Wnt signaling pathways which govern cellular growth control and development. In the TGF-beta pathway KLF5 can antagonize SMAD-mediated transcription suppression promoting cell proliferation particularly in epithelial stem cells. In the Wnt pathway it influences beta-catenin activity essential for epithelial cell renewal and maintenance. Through these pathways KLF5 interacts with proteins such as SMAD3 and beta-catenin coordinating cell turnover and tissue homeostasis.

Associated diseases and disorders

KLF5 impacts cancer and cardiovascular diseases. It plays a complex role in oncogenesis where its overexpression is associated with colorectal breast and prostate cancers due to its ability to enhance proliferation. KLF5's interaction with proteins like p53 influences tumorigenic potentials. In cardiovascular disorders KLF5 contributes to vascular pathology affecting smooth muscle cell proliferation and atherosclerosis. Through its regulation of cyclin D1 it integrates into pathways leading to abnormal vascular smooth muscle growth linking it further to cardiovascular complications.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Western blot - Anti-KLF5 antibody (ab137676)

    All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/5000 dilution

    Lane 1: Non-transfected 293T whole cell extract at 30 µg

    Lane 2: Transfected 293T whole cell extract at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 137 kDa, 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)

    Immunofluorescence analysis of paraformaldehyde-fixed Mouse ESC D3 cells labelling KLF5 using ab137676 at 1/200 dilution. Lower image shows cells co-stained with Hoechst 33342.

  • Western blot - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Western blot - Anti-KLF5 antibody (ab137676)

    All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

    Lane 1: A549 whole cell lysate at 30 µg

    Lane 2: H1299 whole cell lysate at 30 µg

    Lane 3: HCT116 whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KLF5 antibody (ab137676)

    HeLa cells stained for KLF5 (green) using ab137676 at 1/500 dilution in ICC/IF.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody (ab137676)

    ab137676 staining KLF5 in nucleus on HeLa xenograft by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Samples were incubated with primary antibody at a dilution of 1/500

  • Western blot - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Western blot - Anti-KLF5 antibody (ab137676)

    10% SDS PAGE

    All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/1000 dilution

    All lanes: H1299 whole cell lysate at 30 µg

    Predicted band size: 47 kDa, 51 kDa, 53 kDa

  • Western blot - Anti-KLF5 antibody (ab137676), expandable thumbnail

    Western blot - Anti-KLF5 antibody (ab137676)

    Western blot: Anti-KLF5 antibody (ab137676) staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab137676 was shown to bind specifically to KLF5. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in KLF5 knockout cell line. To generate this image, wild-type and KLF5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

    All lanes: Western blot - Anti-KLF5 antibody (ab137676) at 1/2000 dilution

    Lane 1: Wild-type A549 cell lysate at 20 µg

    Lane 2: KLF5 knockout A549 cell lysate at 20 µg

    Lane 3: HAP1 cell lysate at 20 µg

    Lane 4: OVCAR-3 cell lysate at 20 µg

    Lane 5: PANC-1 cell lysate at 20 µg

    Secondary

    All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 55 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com