Anti-KLF5 antibody [BLR243L] - BSA free KO Tested (ab314109)

Knockout Tested Rabbit Recombinant Monoclonal KLF5 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human KLF5 aa 300-350.

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Images

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal

Immunogen

Synthetic Peptide within Human KLF5 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13887

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	Flow Cyt	ICC	IHC-P	IP	WB
Human	Tested	Tested	Tested	Tested	Tested
Mouse	Tested	Tested	Tested	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Target data

See full target information KLF5

Function

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

Alternative names

BTEB2, CKLF, IKLF, KLF5, Krueppel-like factor 5, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, BTE-binding protein 2

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline
Form: Liquid
Clonality: Monoclonal
Immunogen: Synthetic Peptide within Human KLF5 aa 300-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13887
Clone number: BLR243L
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: Up to 12 months
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KLF5 (Kruppel-like factor 5) also referred to as BTEB2 is a transcription factor with a molecular mass of approximately 54 kDa. It belongs to the KLF family characterized by its three C2H2-type zinc finger domains which bind to specific DNA sequences regulating gene expression. KLF5 displays ubiquitous expression but is highly concentrated in epithelial tissues specifically in the gastrointestinal tract and skin. This factor plays an essential role in the regulation of cell proliferation and differentiation.

Biological function summary

Kruppel-like factor 5 modulates gene transcription by binding to GC-rich elements in promoter regions influencing various cellular processes. KLF5 can interact with other transcription factors and co-regulators forming complexes important for its function. It significantly impacts epithelial cell dynamics affecting cell transformation differentiation and migration. In particular it plays a pivotal role in maintaining the proliferative capacity of epithelial cells and supporting wound healing processes.

Pathways

Kruppel-like factor 5 holds a notable position in the TGF-beta and Wnt signaling pathways which govern cellular growth control and development. In the TGF-beta pathway KLF5 can antagonize SMAD-mediated transcription suppression promoting cell proliferation particularly in epithelial stem cells. In the Wnt pathway it influences beta-catenin activity essential for epithelial cell renewal and maintenance. Through these pathways KLF5 interacts with proteins such as SMAD3 and beta-catenin coordinating cell turnover and tissue homeostasis.

Associated diseases and disorders

KLF5 impacts cancer and cardiovascular diseases. It plays a complex role in oncogenesis where its overexpression is associated with colorectal breast and prostate cancers due to its ability to enhance proliferation. KLF5's interaction with proteins like p53 influences tumorigenic potentials. In cardiovascular disorders KLF5 contributes to vascular pathology affecting smooth muscle cell proliferation and atherosclerosis. Through its regulation of cyclin D1 it integrates into pathways leading to abnormal vascular smooth muscle growth linking it further to cardiovascular complications.

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10 product images

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243K] (A700-243) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243K] (A700-243) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L] (A700-243) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution
Lane 1: HeLa Whole cell lysate at 25 µg
Lane 2: SW620 Whole cell lysate at 25 µg
Lane 3: MCF-7 Whole cell lysate at 25 µg
Lane 4: KM12 Whole cell lysate at 25 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit antiKLF5 recombinant monoclonal antibody [BLR243L] (A700-243) used at 1:1000. Secondary :HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].
All lanes: Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution
Lane 1: CT26 Whole cell lysate at 25 µg
Lane 2: WEHI-231 Whole cell lysate at 25 µg
Lane 3: NIH 3T3 Whole cell lysate at 25 µg
Secondary
All lanes: HRP-conjugated goat anti-rabbit IgG
Immunoprecipitation - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L] (A700-243) used for IP at 6 µl/mg lysate. KLF5 was also immunoprecipitated by a second antibody against a different epitope of KLF5 (Antibody 2). For blotting immunoprecipitated KLF5, A700-243 was used at 1:1000. Chemiluminescence with an exposure time of 3 seconds.
Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Western blot: Anti-KLF5 antibody [BLR243L] (ab314109) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab314109 was shown to bind specifically to KLF5. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in KLF5 knockout cell line. To generate this image, wild-type and KLF5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
All lanes: Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: KLF5 knockout A549 cell lysate at 20 µg
Lane 3: HAP1 cell lysate at 20 µg
Lane 4: OVCAR-3 cell lysate at 20 µg
Lane 5: PANC-1 cell lysate at 20 µg
Secondary
All lanes: Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution
Performed under reducing conditions.
Observed band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut.
Antibody: Rabbit antiKLF5 recombinant monoclonal antibody [BLR243L) (A700-243).
Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma.
Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L) (A700-243).
Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells.
Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L) (A700-243).
Secondary: HRP-conjugated goat anti-rabbit IgG.
Immunocytochemistry - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109)
Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells.
Antibody: Rabbit antiKLF5 recombinant monoclonal antibody [BLR243L) (A700-243).
Secondary: HRP-conjugated goat anti-rabbit IgG.