AB314109

Anti-KLF5 antibody [BLR243L] - BSA free

KO Validated
Recombinant
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(1 Publication)

Knockout Tested Rabbit Recombinant Monoclonal KLF5 antibody. Suitable for Flow Cyt, ICC, IHC-P, IP, WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KLF5 aa 300-350.

View Alternative Names

BTEB2, CKLF, IKLF, KLF5, Krueppel-like factor 5, Basic transcription element-binding protein 2, Colon krueppel-like factor, GC-box-binding protein 2, Intestinal-enriched krueppel-like factor, Transcription factor BTEB2, BTE-binding protein 2

11 Images

Supplier Data

Immunocytochemistry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Detection of human KLF5 by immunocytochemistry. Sample : FFPE section of HT-29 cells. Antibody : Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L) (A700-243). Secondary : HRP-conjugated goat anti-rabbit IgG.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Detection of human KLF5 by immunohistochemistry. Sample : FFPE section of human ovarian carcinoma. Antibody : Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L) (A700-243). Secondary : HRP-conjugated goat anti-rabbit IgG.

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody : Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243K] (A700-243) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Supplier Data

Immunoprecipitation - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Samples : Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies : Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L] (A700-243) used for IP at 6 µl/mg lysate. KLF5 was also immunoprecipitated by a second antibody against a different epitope of KLF5 (Antibody 2). For blotting immunoprecipitated KLF5, A700-243 was used at 1 : 1000. Chemiluminescence with an exposure time of 3 seconds.

false

Flow Cyt

Supplier Data

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody : Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243K] (A700-243) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Supplier Data

Immunocytochemistry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Detection of mouse KLF5 by immunocytochemistry. Sample : FFPE section of CT26 cells. Antibody : Rabbit antiKLF5 recombinant monoclonal antibody [BLR243L) (A700-243). Secondary : HRP-conjugated goat anti-rabbit IgG.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Detection of mouse KLF5 by immunohistochemistry. Sample : FFPE section of mouse gut. Antibody : Rabbit antiKLF5 recombinant monoclonal antibody [BLR243L) (A700-243). Secondary : HRP-conjugated goat anti-rabbit IgG.

Supplier Data

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Samples : Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-KLF5 recombinant monoclonal antibody [BLR243L] (A700-243) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution

Lane 1:

HeLa Whole cell lysate at 25 µg

Lane 2:

SW620 Whole cell lysate at 25 µg

Lane 3:

MCF-7 Whole cell lysate at 25 µg

Lane 4:

KM12 Whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Lab

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Western blot : Anti-KLF5 antibody [BLR243L] (ab314109) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in magenta. In Western blot, ab314109 was shown to bind specifically to KLF5. A band was observed at 55 kDa in wild-type A549 cell lysates with no signal observed at this size in KLF5 knockout cell line. To generate this image, wild-type and KLF5 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.

All lanes:

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

KLF5 knockout A549 cell lysate at 20 µg

Lane 3:

HAP1 cell lysate at 20 µg

Lane 4:

OVCAR-3 cell lysate at 20 µg

Lane 5:

PANC-1 cell lysate at 20 µg

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 55 kDa

false

Lab

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Western blot : Anti-KLF5 antibody [BLR243L] - BSA free ab314109 staining at 1/1000 dilution, shown in green; Mouse anti GAPDH (ab8245) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 55 kDa in Wild-type HCT 116 cell lysates with no signal observed at this size in KLF5 knockout HCT 116 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution

Lane 1:

Wild-type HCT 116 at 20 µg

Lane 2:

Western blot - Human KLF5 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-klf5-knockout-hct116-cell-line-ab301030'>ab301030</a>) at 20 µg

Lane 3:

Western blot - Human wild-type A549 cell line (ab288558) at 20 µg

Lane 4:

Western blot - Human KLF5 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-klf5-knockout-a549-cell-line-ab301031'>ab301031</a>) at 20 µg

Secondary

Lanes 1 - 4:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 4:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 51 kDa,55 kDa

Observed band size: 55 kDa,37 kDa

false

Supplier Data

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Samples : Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody : Rabbit antiKLF5 recombinant monoclonal antibody [BLR243L] (A700-243) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F].

All lanes:

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (ab314109) at 1/1000 dilution

Lane 1:

CT26 Whole cell lysate at 25 µg

Lane 2:

WEHI-231 Whole cell lysate at 25 µg

Lane 3:

NIH 3T3 Whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR243L

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

IHC-P, ICC, Flow Cyt, WB, IP

applications

Immunogen

Synthetic Peptide within Human KLF5 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q13887

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

Up to 12 months

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF5 (Kruppel-like factor 5) also referred to as BTEB2 is a transcription factor with a molecular mass of approximately 54 kDa. It belongs to the KLF family characterized by its three C2H2-type zinc finger domains which bind to specific DNA sequences regulating gene expression. KLF5 displays ubiquitous expression but is highly concentrated in epithelial tissues specifically in the gastrointestinal tract and skin. This factor plays an essential role in the regulation of cell proliferation and differentiation.

Biological function summary

Kruppel-like factor 5 modulates gene transcription by binding to GC-rich elements in promoter regions influencing various cellular processes. KLF5 can interact with other transcription factors and co-regulators forming complexes important for its function. It significantly impacts epithelial cell dynamics affecting cell transformation differentiation and migration. In particular it plays a pivotal role in maintaining the proliferative capacity of epithelial cells and supporting wound healing processes.

Pathways

Kruppel-like factor 5 holds a notable position in the TGF-beta and Wnt signaling pathways which govern cellular growth control and development. In the TGF-beta pathway KLF5 can antagonize SMAD-mediated transcription suppression promoting cell proliferation particularly in epithelial stem cells. In the Wnt pathway it influences beta-catenin activity essential for epithelial cell renewal and maintenance. Through these pathways KLF5 interacts with proteins such as SMAD3 and beta-catenin coordinating cell turnover and tissue homeostasis.

KLF5 impacts cancer and cardiovascular diseases. It plays a complex role in oncogenesis where its overexpression is associated with colorectal breast and prostate cancers due to its ability to enhance proliferation. KLF5's interaction with proteins like p53 influences tumorigenic potentials. In cardiovascular disorders KLF5 contributes to vascular pathology affecting smooth muscle cell proliferation and atherosclerosis. Through its regulation of cyclin D1 it integrates into pathways leading to abnormal vascular smooth muscle growth linking it further to cardiovascular complications.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that binds to GC box promoter elements. Activates the transcription of these genes.

See full target information KLF5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26176 PubMed40681764

2025

KLF5-regulated ZDHHC8 reduces chemosensitivity in high-grade serous ovarian cancer through β-catenin signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuowei Xue,Xiao Li,Wanzhen Zhou,Yincheng Teng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLF5 antibody [BLR243L] - BSA free

Immunocytochemistry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Immunoprecipitation - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Flow Cytometry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Immunocytochemistry - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Western blot - Anti-KLF5 antibody [BLR243L] - BSA free (AB314109)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Product promise