AB168527

Anti-KLF8 antibody

Name: Anti-KLF8 antibody (ab168527) | Abcam
Brand: Abcam Limited
SKU: AB168527

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(8 Publications)

Mouse Polyclonal KLF8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLF8.

View Alternative Names

BKLF3, ZNF741, KLF8, Krueppel-like factor 8, Basic krueppel-like factor 3, Zinc finger protein 741

1 Images

Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (AB168527)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (AB168527)

Immunofluorescent analysis of HeLa cells labeling KLF8 with ab168527 at 10 µg/ml.

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KLF8.

O95600

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Chinese hamster": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF8 also known as Kruppel-like factor 8 is a transcription factor involved in regulating gene expression. It contains a zinc finger DNA-binding domain typical for the KLF family and weighs approximately 38 kDa. KLF8 expresses in several tissues with significant presence in kidney lung and liver. It shows strong nuclear localization important for its function in the control of transcriptional tone within cells.

Biological function summary

KLF8 regulates cell proliferation differentiation and development. This protein has been associated with a variety of cellular processes often functioning in cell cycle regulation by modulating the expression of genes involved in cell growth. KLF8 does not function as a part of a larger complex but interacts directly with DNA to exert its effects often affecting other KLF family members like KLF4 and KLF5.

Pathways

KLF8 plays an active role in the TGF-beta signaling and Wnt/beta-catenin pathways. It helps orchestrate cellular responses to growth factors by interacting with these pathways affecting processes such as epithelial-mesenchymal transition (EMT). In these pathways KLF8 associates with proteins such as beta-catenin and SMAD3 further emphasizing its role in cellular proliferation and differentiation.

KLF8 is known to relate to cancer and fibrosis. High KLF8 expression levels have been linked to the aggressiveness of certain cancers such as breast cancer and the protein's action influences EMT a critical step in metastasis. In fibrosis KLF8 contributes to pathological tissue remodeling. It connects with other proteins like MMP9 which are involved in matrix remodeling highlighting its significance in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).

See full target information KLF8

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

eLife 11: PubMed35642785

2022

Differentiated glioma cell-derived fibromodulin activates integrin-dependent Notch signaling in endothelial cells to promote tumor angiogenesis and growth.

Applications

Unspecified application

Species

Unspecified reactive species

Shreoshi Sengupta,Mainak Mondal,Kaval Reddy Prasasvi,Arani Mukherjee,Prerna Magod,Serge Urbach,Dinorah Friedmann-Morvinski,Philippe Marin,Kumaravel Somasundaram

Experimental & molecular medicine 54:47-60 PubMed35031788

2022

Oncogenic E3 ubiquitin ligase NEDD4 binds to KLF8 and regulates the microRNA-132/NRF2 axis in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minghuan Mao,Liang Yang,Jingyao Hu,Bing Liu,Xiling Zhang,Yili Liu,Ping Wang,Hangyu Li

Open life sciences 15:848-859 PubMed33817271

2020

CircSAMD4A contributes to cell doxorubicin resistance in osteosarcoma by regulating the miR-218-5p/KLF8 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Wei,Liefeng Ji,Wanli Duan,Jiang Zhu

Journal of immunology research 2020:4036047 PubMed32411796

2020

miR-24-3p/KLF8 Signaling Axis Contributes to LUAD Metastasis by Regulating EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Pengyu Jing,Nianlin Xie,Nan Zhao,Ximing Zhu,Pei Li,Guizhou Gao,Haizhou Dang,Zhongping Gu

Neoplasma 67:519-527 PubMed32122144

2020

KLF8 promotes cancer stem cell-like phenotypes in osteosarcoma through miR-429-SOX2 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

L Zhang,P Yang,Q Liu,J Wang,F Yan,L Duan,F Lin

Cancer cell international 19:258 PubMed31624471

2019

KLF8 overexpression promotes the growth of human lung cancer cells by promoting the expression of JMJD2A.

Applications

Unspecified application

Species

Unspecified reactive species

Dongjie Ma,Hongsheng Liu,Yingzhi Qin,Zhenhuan Tian,Shanqing Li,Naixin Liang

Journal of experimental & clinical cancer research 37:176 PubMed30055641

2018

Regulation of the adaptation to ER stress by KLF4 facilitates melanoma cell metastasis via upregulating NUCB2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmei Zhang,Jingrong Lin,Yulin Chao,Lu Zhang,Lei Jin,Na Li,Ruiping He,Binbin Ma,Wenzhi Zhao,Chuanchun Han

Biotechnology and applied biochemistry 62:628-33 PubMed25323066

2014

KLF8 is required for bladder cancer cell proliferation and migration.

Applications

Unspecified application

Species

Unspecified reactive species

Kai Liang,Tao Liu,Ning Chu,Jian Kang,Rui Zhang,Yong Yu,Dongqi Li,Dexiang Lu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLF8 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (AB168527)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise