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AB221867

Anti-KLF8 antibody

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Rabbit Polyclonal KLF8 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLF8 aa 50-150.

View Alternative Names

BKLF3, ZNF741, KLF8, Krueppel-like factor 8, Basic krueppel-like factor 3, Zinc finger protein 741

1 Images
Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (AB221867)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (AB221867)

PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for KLF8 (green) using ab221867 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KLF8 aa 50-150. The exact immunogen used to generate this antibody is proprietary information.

O95600

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLF8 also known as Kruppel-like factor 8 is a transcription factor involved in regulating gene expression. It contains a zinc finger DNA-binding domain typical for the KLF family and weighs approximately 38 kDa. KLF8 expresses in several tissues with significant presence in kidney lung and liver. It shows strong nuclear localization important for its function in the control of transcriptional tone within cells.
Biological function summary

KLF8 regulates cell proliferation differentiation and development. This protein has been associated with a variety of cellular processes often functioning in cell cycle regulation by modulating the expression of genes involved in cell growth. KLF8 does not function as a part of a larger complex but interacts directly with DNA to exert its effects often affecting other KLF family members like KLF4 and KLF5.

Pathways

KLF8 plays an active role in the TGF-beta signaling and Wnt/beta-catenin pathways. It helps orchestrate cellular responses to growth factors by interacting with these pathways affecting processes such as epithelial-mesenchymal transition (EMT). In these pathways KLF8 associates with proteins such as beta-catenin and SMAD3 further emphasizing its role in cellular proliferation and differentiation.

KLF8 is known to relate to cancer and fibrosis. High KLF8 expression levels have been linked to the aggressiveness of certain cancers such as breast cancer and the protein's action influences EMT a critical step in metastasis. In fibrosis KLF8 contributes to pathological tissue remodeling. It connects with other proteins like MMP9 which are involved in matrix remodeling highlighting its significance in disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).
See full target information KLF8
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Product promise

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