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Rabbit Polyclonal KLF8 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLF8 aa 50-150.

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Images

Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (AB221867), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Form
Liquid
Clonality
Polyclonal

Immunogen

  • Recombinant Fragment Protein within Human KLF8 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link O95600

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IF
Human
Tested
Mouse
Predicted
Rat
Predicted

Tested
Tested

Species
Human
Dilution info
0.25000-2.00000 µg/mL
Notes

Fixation/Permeabilization: PFA/Triton X-100.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Product

Target data

Function

Transcriptional repressor and activator. Binds to CACCC-boxes promoter elements. Also binds the GT-box of cyclin D1 promoter and mediates cell cycle progression at G(1) phase as a downstream target of focal adhesion kinase (FAK).

Alternative names

BKLF3, ZNF741, KLF8, Krueppel-like factor 8, Basic krueppel-like factor 3, Zinc finger protein 741

Recommended products

Rabbit Polyclonal KLF8 antibody. Suitable for ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLF8 aa 50-150.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Polyclonal
Immunogen
  • Recombinant Fragment Protein within Human KLF8 aa 50-150. The exact immunogen used to generate this antibody is proprietary information. Database link O95600
Purification technique
Affinity purification Immunogen
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KLF8 also known as Kruppel-like factor 8 is a transcription factor involved in regulating gene expression. It contains a zinc finger DNA-binding domain typical for the KLF family and weighs approximately 38 kDa. KLF8 expresses in several tissues with significant presence in kidney lung and liver. It shows strong nuclear localization important for its function in the control of transcriptional tone within cells.

Biological function summary

KLF8 regulates cell proliferation differentiation and development. This protein has been associated with a variety of cellular processes often functioning in cell cycle regulation by modulating the expression of genes involved in cell growth. KLF8 does not function as a part of a larger complex but interacts directly with DNA to exert its effects often affecting other KLF family members like KLF4 and KLF5.

Pathways

KLF8 plays an active role in the TGF-beta signaling and Wnt/beta-catenin pathways. It helps orchestrate cellular responses to growth factors by interacting with these pathways affecting processes such as epithelial-mesenchymal transition (EMT). In these pathways KLF8 associates with proteins such as beta-catenin and SMAD3 further emphasizing its role in cellular proliferation and differentiation.

Associated diseases and disorders

KLF8 is known to relate to cancer and fibrosis. High KLF8 expression levels have been linked to the aggressiveness of certain cancers such as breast cancer and the protein's action influences EMT a critical step in metastasis. In fibrosis KLF8 contributes to pathological tissue remodeling. It connects with other proteins like MMP9 which are involved in matrix remodeling highlighting its significance in disease progression.

Product promise

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1 product image

  • Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (ab221867), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KLF8 antibody (ab221867)

    PFA-fixed, Triton X-100 permeabilized RT4 (human urinary bladder cancer cell line) cells stained for KLF8 (green) using ab221867 at 4 μg/ml in ICC/IF.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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