Anti-KLF9 Antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLF9 Antibody (AB327217)

Immunofluorescent staining of human cell line A-431 using ab327217 at 0.25–2 µg/ml, showing localization to nucleoplasm, plasma membrane & cytosol.

• ChIP

Supplier Data

ChIP - Anti-KLF9 Antibody (AB327217)

ChIP-Exo-Seq composite graph for ab327217 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

• WB

Supplier Data

Western blot - Anti-KLF9 Antibody (AB327217)

Western blot analysis in control (vector only transfected HEK293T lysate) and KLF9 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells) using ab327217 at 0.04–0.4 µg/ml.

All lanes:

Western blot - Anti-KLF9 Antibody (ab327217)

Lane 2:

Control (vector only transfected HEK293T lysate)

Lane 3:

KLF9 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)

Predicted band size: 76.98 kDa

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