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AB203880

Anti-KLHL12 antibody

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(1 Publication)

Rabbit Polyclonal KLHL12 antibody. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KLHL12 aa 50-150 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

C3IP1, KLHL12, Kelch-like protein 12, CUL3-interacting protein 1, DKIR homolog, hDKIR

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL12 antibody (AB203880)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL12 antibody (AB203880)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat tongue, labeling KLHL12 using ab203880 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL12 antibody (AB203880)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL12 antibody (AB203880)

Immunohistochemical analysis of formalin-fixed paraffin-embedded mouse intestine, labeling KLHL12 using ab203880 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Western blot - Anti-KLHL12 antibody (AB203880)
  • WB

Supplier Data

Western blot - Anti-KLHL12 antibody (AB203880)

All lanes:

Western blot - Anti-KLHL12 antibody (ab203880) at 1/200 dilution

Lane 1:

Mouse brain lysates

Lane 2:

Human colon carcinoma lysates

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated

Predicted band size: 63 kDa

Observed band size: 44 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human KLHL12 aa 50-150 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q53G59

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 - 1/200.</p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 - 1/200.</p>" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 - 1/200.</p>" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL12 also known as Kelch-like protein 12 functions mechanically as an E3 ubiquitin ligase. It belongs to the Kelch-like gene family and has a molecular mass of approximately 69 kDa. KLHL12 is expressed in various tissues particularly in the brain and neuronal cells where it plays significant roles in cellular processes. By facilitating protein degradation through ubiquitination KLHL12 regulates protein levels within the cell.
Biological function summary

This target is involved in the regulation of vesicle trafficking and activity through its interaction with various substrates. KLHL12 forms part of a cullin-RING E3 ligase complex which includes the CUL3 protein among others to mediate the ubiquitination of its target proteins. This process influences transport and secretion mechanisms having a critical impact on cellular function and communication.

Pathways

KLHL12 participates in the Wnt signaling pathway and the regulation of endoplasmic reticulum (ER) to Golgi transport. It plays a regulatory role in promoting the secretion of proteins such as collagen by facilitating vesicle formation. KLHL12 interacts with proteins like SEC31 and helps control protein trafficking within the secretory pathway which is essential for maintaining cellular homeostasis and response to external signals.

KLHL12 has links to neurodegenerative diseases and cancer. Abnormal KLHL12 activity or expression can contribute to certain neurodegenerative conditions by impacting neuronal protein homeostasis often associated with protein aggregates. In some cancers either KLHL12 or CUL3 levels are dysregulated potentially driving tumorigenesis by altering cellular growth pathways and protein degradation processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that acts as a negative regulator of Wnt signaling pathway and ER-Golgi transport (PubMed : 22358839, PubMed : 27565346). The BCR(KLHL12) complex is involved in ER-Golgi transport by regulating the size of COPII coats, thereby playing a key role in collagen export, which is required for embryonic stem (ES) cells division : BCR(KLHL12) acts by mediating monoubiquitination of SEC31 (SEC31A or SEC31B) (PubMed : 22358839, PubMed : 27565346). The BCR(KLHL12) complex is also involved in neural crest specification : in response to cytosolic calcium increase, interacts with the heterodimer formed with PEF1 and PDCD6/ALG-2, leading to bridge together the BCR(KLHL12) complex and SEC31 (SEC31A or SEC31B), promoting monoubiquitination of SEC31 and subsequent collagen export (PubMed : 27716508). As part of the BCR(KLHL12) complex, also acts as a negative regulator of the Wnt signaling pathway by mediating ubiquitination and subsequent proteolysis of DVL3 (PubMed : 16547521). The BCR(KLHL12) complex also mediates polyubiquitination of DRD4 and PEF1, without leading to degradation of these proteins (PubMed : 18303015, PubMed : 20100572, PubMed : 27716508).
See full target information KLHL12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Developmental dynamics : an official publication o 248:744-755 PubMed30875130

2019

Expression of new genes in vertebrate tooth development and p63 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Nasim Rostampour,Cassy M Appelt,Aunum Abid,Julia C Boughner
View all publications
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