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AB113906

Anti-KLHL15 antibody

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(1 Publication)

Rabbit Polyclonal KLHL15 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human KLHL15 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

KIAA1677, KLHL15, Kelch-like protein 15

2 Images
Western blot - Anti-KLHL15 antibody (AB113906)
  • WB

Unknown

Western blot - Anti-KLHL15 antibody (AB113906)

Lane 1:

Western blot - Anti-KLHL15 antibody (ab113906) at 1 µg/mL

Lane 2:

Western blot - Anti-KLHL15 antibody (ab113906) at 2 µg/mL

All lanes:

HeLa cell lysate

Predicted band size: 69 kDa

false

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL15 antibody (AB113906)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL15 antibody (AB113906)

ab113906, at 5 μg/ml, staining KLHL15 in formalin-fixed, paraffin-embedded Human Prostate tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human KLHL15 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q96M94

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL15 also known as Kelch-like protein 15 plays an important role in cellular protein dynamics. KLHL15 functions as a substrate-specific adaptor for a Cullin-RING E3 ubiquitin ligase complex which tags proteins for degradation. The mass of KLHL15 is approximately 65 kDa. It is mainly expressed in the central nervous system and several other tissues with varied expression levels across different cell types.
Biological function summary

KLHL15 influences diverse cellular processes through its regulatory role. Acting as part of the ubiquitination machinery KLHL15 aids in maintaining protein homeostasis by targeting specific proteins for ubiquitin-mediated proteasomal degradation. KLHL15 forms part of a complex including Cullin3 and a RING-box protein contributing to protein ubiquitination. This protein interaction ensures proper cellular function by regulating protein turnover.

Pathways

KLHL15 is involved in significant cellular pathways like the ubiquitin-proteasome system and the Wnt signaling pathway. These pathways are important for controlling protein stability and transmitting cell signals. In these pathways KLHL15 interacts with proteins like Cullin3 which are essential for tagging unwanted proteins for elimination thereby affecting various cellular processes.

KLHL15 has associations with neurological diseases and certain types of cancer. In the context of neurodegenerative diseases KLHL15 interacts with proteins such as Cullin3 which are involved in protein degradation pathways impacting disease outcomes. Furthermore alterations in the levels or function of KLHL15 might contribute to the development of specific cancers by disrupting normal protein regulation processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter for CUL3 E3 ubiquitin-protein ligase complex (PubMed : 14528312, PubMed : 27561354, PubMed : 35219381). Acts as an adapter for CUL3 to target the serine/threonine-protein phosphatase 2A (PP2A) subunit PPP2R5B for ubiquitination and subsequent proteasomal degradation, thus promoting exchange with other regulatory subunits (PubMed : 23135275). Acts as an adapter for CUL3 to target the DNA-end resection factor RBBP8/CtIP for ubiquitination and subsequent proteasomal degradation (PubMed : 27561354, PubMed : 35219381). Through the regulation of RBBP8/CtIP protein turnover, plays a key role in DNA damage response, favoring DNA double-strand repair through error-prone non-homologous end joining (NHEJ) over error-free, RBBP8-mediated homologous recombination (HR) (PubMed : 27561354, PubMed : 35219381).
See full target information KLHL15
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cellular and infection microbiology 8:183 PubMed29900129

2018

Peptidase Inhibitor 15 (PI15) Regulates Chlamydial CPAF Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Bhupesh K Prusty,Suvagata R Chowdhury,Nitish Gulve,Thomas Rudel
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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