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AB122164

Anti-KLHL17 antibody

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(1 Publication)

Rabbit Polyclonal KLHL17 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus.

View Alternative Names

AF, KLHL17, Kelch-like protein 17, Actinfilin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-KLHL17 antibody (AB122164)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KLHL17 antibody (AB122164)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122164 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Western blot - Anti-KLHL17 antibody (AB122164)
  • WB

Unknown

Western blot - Anti-KLHL17 antibody (AB122164)

All lanes:

Western blot - Anti-KLHL17 antibody (ab122164) at 1/250 dilution

Lane 1:

RT 4 lysate

Lane 2:

U 251 MG lysate

Lane 3:

Human plasma lysate

Lane 4:

Human liver lysate

Lane 5:

Human tonsil lysate

Predicted band size: 70 kDa

true

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL17 antibody (AB122164)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL17 antibody (AB122164)

ab122164 at 1/100 dilution staining KLHL17 in Paraffin-embedded Human testis tissue by Immunohistochemistry.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q6TDP4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL17 also known as Kelch-like protein 17 has a molecular mass of approximately 68 kDa. This protein belongs to the Kelch-like family characterized by the presence of a BTB/POZ and kelch domains. KLHL17 functions as an adaptor protein linking specific substrate proteins to the ubiquitin-proteasome system for targeted degradation. Expression of KLHL17 occurs mainly in the central nervous system.
Biological function summary

KLHL17 influences neuronal function and development. It forms part of an E3 ubiquitin ligase complex associating with Cullin 3 to facilitate protein ubiquitination. This complex plays a role in synaptic activity by regulating the levels of proteins critical for neuronal signaling. In the brain KLHL17 contributes to the fine-tuning of signaling pathways affecting cognition and memory.

Pathways

KLHL17 integrates into protein degradation and neurodevelopmental signaling pathways. It interacts with components of the ubiquitination pathway primarily linked with Cullin 3. By mediating substrate protein ubiquitination KLHL17 indirectly affects pathways like the Wnt signaling pathway essential for neuronal development. It also cooperates with proteins such as beta-catenin which participates in a wide range of cellular processes.

KLHL17 associates with neurodevelopmental and neurodegenerative diseases. Alterations in KLHL17 function may contribute to conditions such as Alzheimer's disease. Through its role in protein turnover and signaling modulation KLHL17 interacts with other proteins like amyloid precursor protein (APP) which may influence the pathological cascades associated with such disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-recognition component of some cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes. The BCR(KLHL17) complex mediates the ubiquitination and subsequent degradation of GLUR6. May play a role in the actin-based neuronal function (By similarity).
See full target information KLHL17
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Open medicine (Warsaw, Poland) 16:58-67 PubMed33364434

2020

SLC12A8 plays a key role in bladder cancer progression and EMT.

Applications

Unspecified application

Species

Unspecified reactive species

Shun-Lai Li,Zheng-Feng Li,Qing-Wei Cao,Wen-Zhen Wang
View all publications
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Product promise

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