Rabbit Polyclonal KLHL17 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6TDP4
Reactivity data
Select an application| IHC-P | WB | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50.00000 - 1/200.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.04000-0.40000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes - |
Target data
Function
Substrate-recognition component of some cullin-RING-based BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complexes. The BCR(KLHL17) complex mediates the ubiquitination and subsequent degradation of GLUR6. May play a role in the actin-based neuronal function (By similarity).
Alternative names
AF, KLHL17, Kelch-like protein 17, Actinfilin
Recommended products
Rabbit Polyclonal KLHL17 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KLHL17 aa 550 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q6TDP4
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KLHL17 also known as Kelch-like protein 17 has a molecular mass of approximately 68 kDa. This protein belongs to the Kelch-like family characterized by the presence of a BTB/POZ and kelch domains. KLHL17 functions as an adaptor protein linking specific substrate proteins to the ubiquitin-proteasome system for targeted degradation. Expression of KLHL17 occurs mainly in the central nervous system.
- Biological function summary
KLHL17 influences neuronal function and development. It forms part of an E3 ubiquitin ligase complex associating with Cullin 3 to facilitate protein ubiquitination. This complex plays a role in synaptic activity by regulating the levels of proteins critical for neuronal signaling. In the brain KLHL17 contributes to the fine-tuning of signaling pathways affecting cognition and memory.
- Pathways
KLHL17 integrates into protein degradation and neurodevelopmental signaling pathways. It interacts with components of the ubiquitination pathway primarily linked with Cullin 3. By mediating substrate protein ubiquitination KLHL17 indirectly affects pathways like the Wnt signaling pathway essential for neuronal development. It also cooperates with proteins such as beta-catenin which participates in a wide range of cellular processes.
- Associated diseases and disorders
KLHL17 associates with neurodevelopmental and neurodegenerative diseases. Alterations in KLHL17 function may contribute to conditions such as Alzheimer's disease. Through its role in protein turnover and signaling modulation KLHL17 interacts with other proteins like amyloid precursor protein (APP) which may influence the pathological cascades associated with such disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Immunocytochemistry/ Immunofluorescence - Anti-KLHL17 antibody (ab122164)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab122164 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL17 antibody (ab122164)
ab122164 at 1/100 dilution staining KLHL17 in Paraffin-embedded Human testis tissue by Immunohistochemistry.
Western blot - Anti-KLHL17 antibody (ab122164)
All lanes: Western blot - Anti-KLHL17 antibody (ab122164) at 1/250 dilution
Lane 1: RT 4 lysate
Lane 2: U 251 MG lysate
Lane 3: Human plasma lysate
Lane 4: Human liver lysate
Lane 5: Human tonsil lysate
Developed using the ECL technique.
Predicted band size: 70 kDa
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