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AB67682

Anti-KLHL20 antibody

2

(1 Review)

|

(1 Publication)

Mouse Polyclonal KLHL20 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human KLHL20.

View Alternative Names

KLEIP, KLHLX, KLHL20, Kelch-like protein 20, Kelch-like ECT2-interacting protein, Kelch-like protein X

1 Images
Western blot - Anti-KLHL20 antibody (AB67682)
  • WB

Unknown

Western blot - Anti-KLHL20 antibody (AB67682)

All lanes:

Western blot - Anti-KLHL20 antibody (ab67682) at 1/500 dilution

Lane 1:

KLHL20 transfected 293T cell lysate at 25 µg

Lane 2:

Non-transfected 293T cell lysate at 25 µg

Secondary

All lanes:

Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size: 68 kDa

Observed band size: 90 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human KLHL20.

Q9Y2M5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL20 also known as Kelch-like protein 20 is a substrate adaptor protein with a molecular mass of approximately 68 kDa. It is part of the BTB-Kelch protein family and participates in forming E3 ubiquitin ligase complexes. KLHL20 plays a role in mediating the ubiquitination and degradation of protein substrates. This protein is mainly expressed in various tissues including the brain liver and kidney.
Biological function summary

KLHL20 functions importantly in protein homeostasis and cellular regulatory mechanisms. It forms a complex with Cullin 3 and functions as a part of the CRL3 E3 ligase complex where it aids in targeting specific proteins for ubiquitination and degradation. By regulating protein turnover KLHL20 modulates cellular processes such as cell cycle progression and apoptosis.

Pathways

KLHL20 interacts with vital cellular processes such as the ubiquitin-proteasome system (UPS) and oxidative stress response. Its involvement in the UPS facilitates the removal of misfolded proteins helping to maintain cellular protein quality control. KLHL20 interacts closely with proteins like KEAP1 which is a regulator of the Nrf2 pathway therefore playing a role in antioxidative responses.

KLHL20 has implications in cancer and neurodegenerative diseases. Altered expression or function of KLHL20 may lead to disruptions in protein degradation pathways contributing to tumor progression and neuronal damage. In cancer KLHL20's regulatory impact on cell cycle proteins links it with tumor suppressor proteins like p53. In neurodegenerative conditions connections with proteins involved in protein aggregation make KLHL20 an important component in understanding these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex involved in interferon response and anterograde Golgi to endosome transport. The BCR(KLHL20) E3 ubiquitin ligase complex mediates the ubiquitination of DAPK1, leading to its degradation by the proteasome, thereby acting as a negative regulator of apoptosis (PubMed : 20389280). The BCR(KLHL20) E3 ubiquitin ligase complex also specifically mediates 'Lys-33'-linked ubiquitination (PubMed : 24768539). Involved in anterograde Golgi to endosome transport by mediating 'Lys-33'-linked ubiquitination of CORO7, promoting interaction between CORO7 and EPS15, thereby facilitating actin polymerization and post-Golgi trafficking (PubMed : 24768539). Also acts as a regulator of endothelial migration during angiogenesis by controlling the activation of Rho GTPases. The BCR(KLHL20) E3 ubiquitin ligase complex acts as a regulator of neurite outgrowth by mediating ubiquitination and degradation of PDZ-RhoGEF/ARHGEF11 (PubMed : 21670212). In case of tumor, the BCR(KLHL20) E3 ubiquitin ligase complex is involved in tumor hypoxia : following hypoxia, the BCR(KLHL20)complex mediates ubiquitination and degradation of PML, potentiating HIF-1 signaling and cancer progression (PubMed : 21840486).
See full target information KLHL20
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular cell 68:615-625.e9 PubMed29056324

2017

The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ivana Horvathova,Franka Voigt,Anna V Kotrys,Yinxiu Zhan,Caroline G Artus-Revel,Jan Eglinger,Michael B Stadler,Luca Giorgetti,Jeffrey A Chao
View all publications
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