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AB111604

Anti-KLHL21 antibody

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(2 Publications)

Rabbit Polyclonal KLHL21 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL21 aa 300-550.

View Alternative Names

KIAA0469, KLHL21, Kelch-like protein 21

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL21 antibody (AB111604)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL21 antibody (AB111604)

ab111604, at a 1/500, staining KLHL21 in paraffin-embedded Hepatoma tissue.

Western blot - Anti-KLHL21 antibody (AB111604)
  • WB

Unknown

Western blot - Anti-KLHL21 antibody (AB111604)

7.5% SDS PAGE

All lanes:

Western blot - Anti-KLHL21 antibody (ab111604) at 1/1000 dilution

All lanes:

A549 whole cell lysate at 30 µg

Predicted band size: 55 kDa,58 kDa,61 kDa,62 kDa,63 kDa,64 kDa,67 kDa,82 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human KLHL21 aa 300-550. The exact immunogen used to generate this antibody is proprietary information.

Q9UJP4

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>Perform heat mediated antigen retrieval with 10mM citrate buffer pH 6.0 or Tris-EDTA buffer pH 8.0 before commening with IHC staining protocol.</p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL21 also known as Kelch-like protein 21 is a part of the BTB-Kelch protein family. It is approximately 64 kDa in mass and plays a role in protein-protein interactions. KLHL21 acts as an adaptor that recruits specific substrates for ubiquitination. This protein has high expression levels in testis and moderate expression in various other tissues including the heart and kidney. Varying expression levels suggest a functional versatility across different cellular environments.
Biological function summary

KLHL21 forms part of the Cul3-based E3 ubiquitin ligase complex. This complex targets proteins for ubiquitination which marks them for degradation via the proteasome pathway. By mediating targeted protein turnover KLHL21 maintains cellular protein homeostasis. This is critical for regulating cell cycle progression and apoptosis highlighting its functional importance in maintaining cell integrity and function.

Pathways

KLHL21 integrates into cellular mechanisms through the ubiquitin-proteasome pathway. Its activity impacts protein degradation an important component of cellular regulation and signaling. KLHL21 interacts with important proteins like CUL3 part of the ubiquitin ligase complex assisting in the degradation of misfolded or damaged proteins. Proper function of this pathway is important for responding to cellular stress and maintaining cellular health.

KLHL21 links to various cancers due to its role in cell cycle regulation and apoptosis. The improper function or expression of KLHL21 can contribute to uncontrolled cell proliferation and tumor development. Additionally its interaction with CUL3 implicates KLHL21 in disorders like neurodegenerative diseases where ubiquitination and protein turnover are disrupted. Understanding KLHL21's role in these processes could aid in developing therapeutic approaches targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin-protein ligase complex required for efficient chromosome alignment and cytokinesis. The BCR(KLHL21) E3 ubiquitin ligase complex regulates localization of the chromosomal passenger complex (CPC) from chromosomes to the spindle midzone in anaphase and mediates the ubiquitination of AURKB. Ubiquitination of AURKB by BCR(KLHL21) E3 ubiquitin ligase complex may not lead to its degradation by the proteasome.
See full target information KLHL21
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Journal of investigative dermatology 141:894-902.e9 PubMed33069730

2020

EZH2 Regulates the Correlation between Skin Regeneration and the Duration of Mechanical Stretch.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Wang,Yifan Zhang,Ya Gao,Shengzhou Shan,Qingfeng Li

The Journal of biological chemistry 291:18176-89 PubMed27387502

2016

Kelch-like Protein 21 (KLHL21) Targets IκB Kinase-β to Regulate Nuclear Factor κ-Light Chain Enhancer of Activated B Cells (NF-κB) Signaling Negatively.

Applications

Unspecified application

Species

Unspecified reactive species

Zhu-Zhong Mei,Xin-Yu Chen,Shui-Wang Hu,Ni Wang,Xiao-Li Ou,Jing Wang,Hai-Hua Luo,Jinghua Liu,Yong Jiang
View all publications
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Product promise

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