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AB229246

Anti-KLHL25 antibody - C-terminal

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(1 Publication)

Rabbit Polyclonal KLHL25 antibody. C-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL25.

View Alternative Names

ENC2, KLHL25, Kelch-like protein 25, Ectoderm-neural cortex protein 2, ENC-2

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)

Paraffin-embedded human colon carcinoma tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-KLHL25 antibody - C-terminal (AB229246)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLHL25 antibody - C-terminal (AB229246)

Methanol-fixed MCF7 (human breast adenocarcinoma cell line) cells stained for KLHL25 (green) using ab229246 at 1/200 dilution in ICC/IF.

Blue : Hoechst 33342 staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)

Paraffin-embedded mouse muscle tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)

Paraffin-embedded mouse testis tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL25 antibody - C-terminal (AB229246)

Paraffin-embedded rat fore brain tissue stained for KLHL25 using ab229246 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-KLHL25 antibody - C-terminal (AB229246)
  • WB

Supplier Data

Western blot - Anti-KLHL25 antibody - C-terminal (AB229246)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-KLHL25 antibody - C-terminal (ab229246) at 1/1000 dilution

All lanes:

NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) whole cell lysate at 30 µg

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KLHL25. The exact immunogen used to generate this antibody is proprietary information.

Q9H0H3

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein KLHL25 also known as Kelch-like protein 25 is a member of the Kelch-like (KLHL) family. It has a mass of approximately 64 kDa. KLHL25 contains Kelch domains that participate in protein-protein interactions by forming beta-propeller structures. Expressed in various tissues including the brain liver and kidney KLHL25 functions as a substrate-specific adapter within E3 ubiquitin ligase complexes targeting specific proteins for ubiquitination and subsequent degradation by the proteasome.
Biological function summary

KLHL25 interacts with target proteins through its Kelch domains facilitating their ubiquitination as part of an E3 ligase complex. It participates in the fine-tuning of cellular processes by controlling protein turnover. Additionally KLHL25 plays a role in cytoskeletal organization by interacting with actin-binding proteins. These functions contribute to maintaining cellular homeostasis impacting cell signaling and structural integrity.

Pathways

KLHL25 integrates into cellular ubiquitination pathways that govern protein degradation. Its interaction affects signaling pathways such as the Wnt signaling pathway which is important for cell development and growth. KLHL25 likely works in concert with other E3 ligase components including CUL3 a scaffold protein that helps in forming multi-protein E3 ligase assemblies. The combined actions within these pathways help maintain balance in the presence of cellular stress or growth signals.

KLHL25 links to neurodegenerative diseases one being Parkinson’s disease through its role in protein degradation pathways. It potentially affects the stability of proteins like alpha-synuclein which aggregates in Parkinson's disease. Abnormal function or expression of KLHL25 may contribute to the accumulation of misfolded proteins disrupting normal cell function and leading to neurodegeneration. Understanding KLHL25’s role in these contexts could offer insights into therapeutic strategies for managing such conditions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex involved in various processes, such as translation homeostasis and lipid synthesis (PubMed : 22578813, PubMed : 27664236, PubMed : 34491895). The BCR(KLHL25) ubiquitin ligase complex acts by mediating ubiquitination of hypophosphorylated EIF4EBP1 (4E-BP1) : ubiquitination and subsequent degradation of hypophosphorylated EIF4EBP1 (4E-BP1) probably serves as a homeostatic mechanism to maintain translation and prevent eIF4E inhibition when eIF4E levels are low (PubMed : 22578813). The BCR(KLHL25) complex does not target EIF4EBP1 (4E-BP1) when it is hyperphosphorylated or associated with eIF4E (PubMed : 22578813). The BCR(KLHL25) complex also acts as a regulator of lipid synthesis by mediating ubiquitination and degradation of ACLY, thereby inhibiting lipid synthesis (PubMed : 27664236, PubMed : 34491895). BCR(KLHL25)-mediated degradation of ACLY promotes fatty acid oxidation and is required for differentiation of inducible regulatory T (iTreg) cells (PubMed : 34491895).
See full target information KLHL25
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular and molecular medicine 28:e70079 PubMed39300613

2024

Multi-dataset identification of innovative feature genes and molecular mechanisms in keratoconus.

Applications

Unspecified application

Species

Unspecified reactive species

Ning Lyu,Yiqin Dai,Jiawen Wu,Yidan Fan,Zhaoyuan Lyu,Jiayu Gu,Jingyi Cheng,Jianjiang Xu
View all publications
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