AB243490

Anti-KLHL35 antibody

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(2 Publications)

Rabbit Polyclonal KLHL35 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KLHL35 aa 400-550.

View Alternative Names

Kelch-like protein 35, KLHL35

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KLHL35 antibody (AB243490)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLHL35 antibody (AB243490)

PFA-fixed, Triton X-100 permeabilized SiHa cells stained for KLHL35 (green) using ab243490 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL35 antibody (AB243490)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL35 antibody (AB243490)

Paraffin-embedded human kidney tissue stained for KLHL35 using ab243490 at 1/50 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KLHL35 aa 400-550. The exact immunogen used to generate this antibody is proprietary information.

Q6PF15

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL35 also known as Kelch-like protein 35 is a protein encoded by the KLHL35 gene in humans. It consists of 615 amino acids leading to a molecular mass of approximately 68 kDa. This protein contains a BACK domain a BTB domain and several Kelch repeats. KLHL35 is expressed in various tissues with notable expression levels in the kidneys and liver. Its structural domains suggest roles in protein-protein interactions and cellular organization.

Biological function summary

KLHL35 functions as part of an E3 ubiquitin ligase complex. Key activities of this complex include tagging specific proteins for degradation via the ubiquitin-proteasome system. By directing the proteolytic pathways KLHL35 plays a part in the regulation of protein turnover and cellular homeostasis. Its presence within this complex indicates a role in maintaining cellular protein quality control influencing various cellular processes.

Pathways

KLHL35 significantly contributes to the ubiquitin-proteasome pathway. It interacts with various proteins by facilitating their ubiquitination and subsequent degradation. Through these interactions KLHL35 influences essential cellular processes linked to protein homeostasis. Another pathway where KLHL35 plays a role is the cell cycle regulation interacting with proteins such as CUL3. These pathways highlight KLHL35's engagement in maintaining cellular functions and protein regulation.

KLHL35 has possible connections to certain kidney disorders due to its expression in renal tissues. Its role in protein degradation suggests that mutations or dysregulation in KLHL35 could affect cellular homeostasis. Additionally aberrant KLHL35 functioning is potentially linked to liver-related conditions. Alterations in the ubiquitination pathways may result in the accumulation or misfolding of proteins that contribute to these ailments possibly involving proteins like p53 which are known for cellular stress response.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

See full target information KLHL35

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Tohoku journal of experimental medicine : PubMed41062300

2025

Identification of KLHL35, WDR72, and WDR78 as Prognostic Biomarkers in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayou Ye,Tingting Zhang,Guangsheng Wang,Guofeng Bian,Aijun Chen,Xin Zhou

Journal of translational medicine 22:216 PubMed38424632

2024

Ubiquitylation of RUNX3 by RNA-binding ubiquitin ligase MEX3C promotes tumorigenesis in lung adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zelai He,Huijun Zhang,Haibo Xiao,Xiangyu Zhang,Hongbo Xu,Ruifen Sun,Siwen Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLHL35 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KLHL35 antibody (AB243490)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KLHL35 antibody (AB243490)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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