JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB182163

Anti-KLHL6 antibody [EPR13484]

5

(2 Reviews)

|

(2 Publications)

Rabbit Recombinant Monoclonal KLHL6 antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Transfected cell line - Human samples. Cited in 2 publications.

View Alternative Names

Kelch-like protein 6, KLHL6

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] (AB182163)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] (AB182163)

Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.

Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] (AB182163)
  • IP

Supplier Data

Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] (AB182163)

Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using ab182163 at 1/50 dilution.

Secondary : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] (ab182163)

Predicted band size: 70 kDa

false

Western blot - Anti-KLHL6 antibody [EPR13484] (AB182163)
  • WB

Supplier Data

Western blot - Anti-KLHL6 antibody [EPR13484] (AB182163)

All lanes:

Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution

All lanes:

A549 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Western blot - Anti-KLHL6 antibody [EPR13484] (AB182163)
  • WB

Supplier Data

Western blot - Anti-KLHL6 antibody [EPR13484] (AB182163)

All lanes:

Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Western blot - Anti-KLHL6 antibody [EPR13484] (AB182163)
  • WB

Supplier Data

Western blot - Anti-KLHL6 antibody [EPR13484] (AB182163)

Blocking buffer and concentration :  5% NFDM/TBST  Diluting buffer and concentration :  5% NFDM/TBST Exposure Time : Lane 1-2 : 1 second, Lane 3 : 40 seconds. ab181602 was used as loading controls. ab205606 was used as a total protein control. Tag constructed at C -terminal of overexpression vector may interfere the recognition of this antibody to targeted protein.

All lanes:

Western blot - Anti-KLHL6 antibody [EPR13484] (ab182163) at 1/1000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control) whole cell lysate at 20 µg

Lane 2:

293T transfected with KLHL6 expression vector containing a N-terminal DDDDK tag, whole cell lysate at 20 µg

Lane 3:

Human tonsil lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 70 kDa

Observed band size: 70 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13484

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" }, "Transfected cell line - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLHL6 also known as Kelch-like protein 6 is a member of the Kelch-like family of proteins. It plays a mechanistic role in protein-protein interactions mainly by acting as a substrate recognition component of an E3 ubiquitin ligase complex. KLHL6 has a molecular mass of approximately 52 kDa. This protein is primarily expressed in B cells and it is most abundant in the germinal center B cells within the lymphoid tissues.
Biological function summary

KLHL6 contributes to the regulation of B cell maturation and differentiation. Research indicates that KLHL6 is part of a Cul3-based E3 ubiquitin ligase complex which involves protein degradation processes. By functioning in this complex KLHL6 interacts with other proteins to ensure proper B cell development and immune responses. This function is important for maintaining normal immune system activities.

Pathways

KLHL6 plays a significant role in the B cell receptor (BCR) signaling pathway. This pathway is essential for B cell activation and differentiation. KLHL6 is associated with other pathway-related proteins such as BCR components and signaling molecules downstream in the pathway. Another key biological pathway involving KLHL6 is the ubiquitin-proteasome system which regulates protein turnover and influences various cellular processes by targeting proteins for degradation.

KLHL6 alteration has been linked to certain B cell malignancies such as chronic lymphocytic leukemia (CLL). Its expression levels or mutations might influence the progression of these diseases. The protein is also connected with other ubiquitin-proteasome pathway components such as the Cul3 E3 ligase complex which might affect these disorders' development. Understanding KLHL6's role in these contexts can provide insights into potential therapeutic targets.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Involved in B-lymphocyte antigen receptor signaling and germinal center formation.
See full target information KLHL6
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Blood 142:973-988 PubMed37235754

2023

DLBCL-associated NOTCH2 mutations escape ubiquitin-dependent degradation and promote chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Zhou,Jaewoo Choi,Grant Grothusen,Bang-Jin Kim,Diqiu Ren,Zhendong Cao,Yiman Liu,Qinglan Li,Arati Inamdar,Thomas Beer,Hsin-Yao Tang,Eric Perkey,Ivan Maillard,Roberto Bonasio,Junwei Shi,Marco Ruella,Liling Wan,Luca Busino

Nature cell biology 20:586-596 PubMed29695787

2018

Loss of KLHL6 promotes diffuse large B-cell lymphoma growth and survival by stabilizing the mRNA decay factor roquin2.

Applications

Unspecified application

Species

Unspecified reactive species

Jaewoo Choi,Kyutae Lee,Kristin Ingvarsdottir,Roberto Bonasio,Anita Saraf,Laurence Florens,Michael P Washburn,Saber Tadros,Michael R Green,Luca Busino
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB254776

Anti-ZNF711 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ZNF711 antibody (AB254776)

  • 0
  • 0
Primary Antibodies

AB125084

Anti-C14orf133 antibody

Immunoprecipitation - Anti-C14orf133 antibody (AB125084)

  • 0
  • 0
Primary Antibodies

AB316194

Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free

BOND RX™ Validated|Recombinant|RabMAb

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SLC6A14 antibody [EPR27113-178] - BSA and Azide free (AB316194)

  • 0
  • 0
Primary Antibodies

AB181064

Anti-PFKM + PFKL antibody [EPR11904]

RabMAb|Recombinant|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-PFKM + PFKL antibody [EPR11904] (AB181064)

  • 5
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com