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Rabbit Recombinant Monoclonal KLHL6 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Transfected cell line - Human samples.

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Images

Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (AB250605), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (AB250605), expandable thumbnail
  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (AB250605), expandable thumbnail
  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (AB250605), expandable thumbnail
  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (AB250605), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWB
Human
Tested
Tested
Tested
Transfected cell line - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Transfected cell line - Human, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

1 products for Alternative Product

Target data

Function

Involved in B-lymphocyte antigen receptor signaling and germinal center formation.

Alternative names

Kelch-like protein 6, KLHL6

Recommended products

Rabbit Recombinant Monoclonal KLHL6 antibody. Carrier free. Suitable for ICC/IF, IP, WB and reacts with Human, Transfected cell line - Human samples.

Alternative names

Kelch-like protein 6, KLHL6

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR13484

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab250605 is the carrier-free version of Anti-KLHL6 antibody [EPR13484] ab182163.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KLHL6 also known as Kelch-like protein 6 is a member of the Kelch-like family of proteins. It plays a mechanistic role in protein-protein interactions mainly by acting as a substrate recognition component of an E3 ubiquitin ligase complex. KLHL6 has a molecular mass of approximately 52 kDa. This protein is primarily expressed in B cells and it is most abundant in the germinal center B cells within the lymphoid tissues.

Biological function summary

KLHL6 contributes to the regulation of B cell maturation and differentiation. Research indicates that KLHL6 is part of a Cul3-based E3 ubiquitin ligase complex which involves protein degradation processes. By functioning in this complex KLHL6 interacts with other proteins to ensure proper B cell development and immune responses. This function is important for maintaining normal immune system activities.

Pathways

KLHL6 plays a significant role in the B cell receptor (BCR) signaling pathway. This pathway is essential for B cell activation and differentiation. KLHL6 is associated with other pathway-related proteins such as BCR components and signaling molecules downstream in the pathway. Another key biological pathway involving KLHL6 is the ubiquitin-proteasome system which regulates protein turnover and influences various cellular processes by targeting proteins for degradation.

Associated diseases and disorders

KLHL6 alteration has been linked to certain B cell malignancies such as chronic lymphocytic leukemia (CLL). Its expression levels or mutations might influence the progression of these diseases. The protein is also connected with other ubiquitin-proteasome pathway components such as the Cul3 E3 ligase complex which might affect these disorders' development. Understanding KLHL6's role in these contexts can provide insights into potential therapeutic targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605), expandable thumbnail

    Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

    This data was developed using Anti-KLHL6 antibody [EPR13484] ab182163, the same antibody clone in a different buffer formulation.Western blot analysis of immunoprecipitation pellet from A549 cell lysate immunoprecipitated using Anti-KLHL6 antibody [EPR13484] ab182163 at 1/50 dilution.

    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    All lanes: Immunoprecipitation - Anti-KLHL6 antibody [EPR13484] (Anti-KLHL6 antibody [EPR13484] ab182163)

    Predicted band size: 70 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

    This data was developed using Anti-KLHL6 antibody [EPR13484] ab182163, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling KLHL6 with Anti-KLHL6 antibody [EPR13484] ab182163 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution. Counter stained with DAPI.

  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605), expandable thumbnail

    Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

    This data was developed using Anti-KLHL6 antibody [EPR13484] ab182163, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-KLHL6 antibody [EPR13484] (Anti-KLHL6 antibody [EPR13484] ab182163) at 1/1000 dilution

    All lanes: A549 cell lysate at 10 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 70 kDa

    Observed band size: 70 kDa

  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605), expandable thumbnail

    Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

    This data was developed using Anti-KLHL6 antibody [EPR13484] ab182163, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-KLHL6 antibody [EPR13484] (Anti-KLHL6 antibody [EPR13484] ab182163) at 1/1000 dilution

    All lanes: Human fetal kidney lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution

    Predicted band size: 70 kDa

    Observed band size: 70 kDa

  • Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605), expandable thumbnail

    Western blot - Anti-KLHL6 antibody [EPR13484] - BSA and Azide free (ab250605)

    Blocking buffer and concentration: 5% NFDM/TBST 

    Diluting buffer and concentration: 5% NFDM/TBST

    Exposure Time: Lane 1-2: 1 second, Lane 3: 40 seconds.

    Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 was used as loading controls. Anti-DDDDK tag (Binds to FLAG® tag sequence) antibody [EPR20018-251] ab205606 was used as a total protein control.

    Tag constructed at C -terminal of overexpression vector may interfere the recognition of this antibody to targeted protein.

    This data was developed using Anti-KLHL6 antibody [EPR13484] ab182163, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-KLHL6 antibody [EPR13484] (Anti-KLHL6 antibody [EPR13484] ab182163) at 1/1000 dilution

    Lane 1: 293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control) whole cell lysate at 20 µg

    Lane 2: 293T transfected with KLHL6 expression vector containing a N-terminal DDDDK tag, whole cell lysate at 20 µg

    Lane 3: Human tonsil lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 70 kDa

    Observed band size: 70 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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