AB197892

Anti-KLLN antibody [EPR16934]

RabMAb
Recombinant
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(4 Publications)

Rabbit Recombinant Monoclonal KLLN antibody. Suitable for WB and reacts with Rat, Human, Mouse samples. Cited in 4 publications.

View Alternative Names

Killin, KLLN

3 Images

Supplier Data

Western blot - Anti-KLLN antibody [EPR16934] (AB197892)

Blocking/dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilution

Lane 1:

Human fetal brain lysate at 10 µg

Lane 2:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 20 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KLLN antibody [EPR16934] (AB197892)

All lanes:

Western blot - Anti-KLLN antibody [EPR16934] (ab197892) at 1/2000 dilution

Lane 1:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 3:

HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-KLLN antibody [EPR16934] (AB197892)

All lanes:

Western blot - Anti-KLLN antibody [EPR16934] (ab197892) at 1/1000 dilution

Lane 1:

C6 (Rat glial tumor cells) whole cell lysate at 10 µg

Lane 2:

Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Lane 3:

PC12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

Lane 4:

NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16934

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLLN also known as Killin is a DNA-binding protein approximately 19 kDa in size. It is expressed mainly in the nucleus of cells. KLLN plays a role in DNA replication inhibition and is involved in apoptosis. Its expression level may vary among different tissues with a reported presence in the thymus spleen and bone marrow. The connection of KLLN to certain cellular processes highlights its significance for maintaining cellular homeostasis and regulating cell growth.

Biological function summary

Killin functions as a direct inhibitor of DNA synthesis. It interacts with components involved in cell cycle control serving as a putative tumor suppressor. Killin can form part of larger protein complexes influencing the DNA damage response by mediating G1 arrest which is critical to prevent uncontrolled cell division. This protein exerts its effects by managing the balance between cell proliferation and programmed cell death making it a point of interest for researchers studying carcinogenesis mechanisms.

Pathways

We observe that Killin integrates into the p53 signaling pathway an important pathway in the regulation of cell growth and apoptosis. It is closely associated with proteins like p53 and PTEN. The KLLN gene lies adjacent to PTEN and these proteins often co-regulate each other’s activity impacting key cellular events such as stress response and DNA damage repair. This alignment with the p53 and PTEN pathways highlights Killin's role in maintaining genomic integrity and cellular responses to damage.

Killin displays a notable connection with certain cancers and neurodegenerative disorders. Alterations in its expression or mutations can influence the development of prostate cancer with its role as a tumor suppressor directly impacting cellular proliferation pathways. Additionally KLLN’s interaction with PTEN links it to the genetic disorder Cowden syndrome which features increased cancer risk and skin lesions. Understanding KLLN's involvement with these diseases offers insight into potential therapeutic targets and new strategies for treatment interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

DNA-binding protein involved in S phase checkpoint control-coupled apoptosis by mediating p53/TP53-induced apoptosis. Has the ability to inhibit DNA synthesis and S phase arrest coupled to apoptosis. Has affinity to both double- and single-stranded DNA.

See full target information Killin

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Genome biology 24:248 PubMed37904237

2023

Landscape of enhancer disruption and functional screen in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Wang,Menghan Luo,Qian Liang,Ke Zhao,Yuelin Hu,Wei Wang,Xiangling Feng,Bolang Hu,Jianjin Teng,Tianyi You,Ran Li,Zhengkai Bao,Wenhao Pan,Tielong Yang,Chao Zhang,Ting Li,Xiaobao Dong,Xianfu Yi,Ben Liu,Li Zhao,Miaoxin Li,Kexin Chen,Weihong Song,Jilong Yang,Mulin Jun Li

Cell death & disease 13:800 PubMed36123344

2022

LncRNA SEMA3B-AS1 inhibits breast cancer progression by targeting miR-3940/KLLN axis.

Applications

Unspecified application

Species

Unspecified reactive species

Jin Hu,Haohao Huang,Zihan Xi,Shenghui Ma,Jie Ming,Fang Dong,Hui Guo,Huiqiong Zhang,Ende Zhao,Guojie Yao,Liu Yang,Feng Zhang,Wuping Zheng,Hengyu Chen,Tao Huang,Lei Li

Experimental and therapeutic medicine 18:3299-3306 PubMed31602202

2019

MicroRNA-204 may participate in the pathogenesis of hypoxic-ischemic encephalopathy through targeting KLLN.

Applications

Unspecified application

Species

Unspecified reactive species

Ronglin Chen,Meixia Wang,Shaopin Fu,Feng Cao,Pengkai Duan,Jiefu Lu

Molecular therapy. Nucleic acids 17:563-577 PubMed31382188

2019

LINC00472 Acts as a Tumor Suppressor in NSCLC through KLLN-Mediated p53-Signaling Pathway via MicroRNA-149-3p and MicroRNA-4270.

Applications

Unspecified application

Species

Unspecified reactive species

Aimei Zou,Xingli Liu,Zongjiong Mai,Junke Zhang,Zhuohuan Liu,Qilu Huang,Aibing Wu,Chenyu Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KLLN antibody [EPR16934]

Western blot - Anti-KLLN antibody [EPR16934] (AB197892)

Western blot - Anti-KLLN antibody [EPR16934] (AB197892)

Western blot - Anti-KLLN antibody [EPR16934] (AB197892)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise