AB203576

Anti-Klotho antibody

Name: Anti-Klotho antibody (ab203576) | Abcam
Brand: Abcam Limited
SKU: AB203576
Rating: 5 (2 reviews)

(2 Reviews)

(25 Publications)

Rabbit Polyclonal Klotho antibody. Suitable for IHC-P and reacts with Mouse, Rat samples. Cited in 25 publications. Immunogen corresponding to Synthetic Peptide within Human KL aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Klotho, KL

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (AB203576)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (AB203576)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded rat kidney tissue labeling Klotho with ab203576 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (AB203576)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse kidney tissue labeling Klotho with ab203576 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human KL aa 400 to C-terminus conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q9UEF7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Klotho also known as alpha Klotho protein is a multifunctional protein prominently involved in regulating aging processes. It consists of approximately 130 kDa and is expressed mainly in the kidneys brain and parathyroid gland. This membrane-bound protein may also exist in a soluble form after cleavage by specific enzymes. Alpha Klotho functions as a co-receptor for fibroblast growth factor 23 (FGF23) facilitating phosphate and vitamin D metabolism regulation.

Biological function summary

Alpha Klotho influences several cellular processes such as aging and mineral ion homeostasis. As a part of the FGF23-Klotho complex it enables the fibroblast growth factor to exert its biological effects. This protein modulates the effects of FGF23 on the renal excretion of phosphate and the synthesis of vitamin D. By interacting with FGF23 alpha Klotho supports maintaining electrolyte balance vital for proper physiological functioning.

Pathways

The FGF23-Klotho axis integrates into significant signaling pathways regulating mineral metabolism and aging. Klotho protein interactions with FGF23 contribute to controlling phosphate homeostasis by inhibiting renal phosphate reabsorption and suppressing vitamin D synthesis. It also engages with other proteins notably the fibroblast growth factors to regulate aging-related pathways ensuring cellular longevity and repair mechanisms are optimized.

Understanding Klotho's role helps in managing conditions like chronic kidney disease and some neurological disorders. Research indicates that reduced levels of alpha Klotho may correspond to kidney dysfunction due to its critical role in mineral metabolism. Furthermore alterations in Klotho expression connect with neurodegenerative diseases implicating its interaction with other proteins such as amyloid precursor protein and tau which are significant in disorders like Alzheimer's disease.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity).. The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

See full target information KL

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Publications (25)

Recent publications for all applications. Explore the full list and refine your search

BMC cardiovascular disorders 25:492 PubMed40618025

2025

Klotho modulation by tempol: a potential therapeutic axis in sepsis.

Applications

Unspecified application

Species

Unspecified reactive species

Alaa Al-Kadi,Aliaa F Anter,Remon Roshdy Rofaeil,Mohamed M Sayed-Ahmed,Sara Mohamed Naguib Abdel Hafez,Al-Shaimaa F Ahmed

Cellular and molecular biology (Noisy-le-Grand, France) 69:109-117 PubMed37715411

2023

Bisphenol-A exposure alters liver, kidney, and pancreatic Klotho expression by HSP60-activated mTOR/autophagy pathway in male albino rats.

Applications

Unspecified application

Species

Unspecified reactive species

Ahmed A Morsi,Ezat A Mersal,Ahmed Othman Alsabih,Shaimaa Alakabawy,Riham G Elfawal,Eman M Sakr,Eman Mohamed Faruk,Mohammed Abu-Rashed,Fatimah Alhaddad,Zahra Alkhawajah,Abdulrahman Hasari,Khalid Elfaki Ibrahim Ibrahim,Ahmed M Abdelmoneim

International journal of medical sciences 20:810-817 PubMed37213666

2023

Recombinant Klotho attenuates IFNγ receptor signaling and SAMHD1 expression through blocking NF-κB translocation in glomerular mesangial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Kuen-Daw Tsai,Yi-Chao Lee,Bo-Yu Chen,Li-Syuan Wu,Shan-Yuan Liang,Ming-Yuan Liu,Yu-Wen Hung,Hui-Ling Hsu,Pei-Qi Chen,Jia-Ching Shieh,Yi-Ju Lee,Ting-Hui Lin

Biomedicines 11: PubMed36831102

2023

Hydrogen Sulfide Inhibited Sympathetic Activation in D-Galactose-Induced Aging Rats by Upregulating Klotho and Inhibiting Inflammation in the Paraventricular Nucleus.

Applications

Unspecified application

Species

Unspecified reactive species

Hao Yu,Qiyao Yu,Yuan Mi,Ping Wang,Sheng Jin,Lin Xiao,Qi Guo,Yuming Wu

International journal of molecular sciences 24: PubMed36674721

2023

Klotho Regulated by Estrogen Plays a Key Role in Sex Differences in Stress Resilience in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhinei Tan,Yongxia Li,Yinzheng Guan,Javed Iqbal,Chenyue Wang,Riqiang Yan,Xin-Ming Ma

International journal of molecular sciences 23: PubMed36362179

2022

Therapeutic Ultrasound Halts Progression of Chronic Kidney Disease In Vivo via the Regulation of Markers Associated with Renal Epithelial-Mesenchymal Transition and Senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Chen-Yu Lin,Ching-Chia Wang,Jui-Zhi Loh,Tsai-Chen Chiang,Te-I Weng,Ding-Cheng Chan,Kuan-Yu Hung,Chih-Kang Chiang,Shing-Hwa Liu

Frontiers in rehabilitation sciences 2:807123 PubMed36188832

2022

Klotho: An Emerging Factor With Ergogenic Potential.

Applications

Unspecified application

Species

Unspecified reactive species

Eliott Arroyo,Ashley D Troutman,Ranjani N Moorthi,Keith G Avin,Andrew R Coggan,Kenneth Lim

Frontiers in immunology 13:934243 PubMed36189255

2022

Comprehensive analysis of cellular senescence-related genes in the prognosis, tumor microenvironment, and immunotherapy/chemotherapy of clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Caibao Lu,Yiqin Wang,Ling Nie,Liping Chen,Moqi Li,Huimin Qing,Sisi Li,Shuang Wu,Zhe Wang

American journal of translational research 14:1234-1245 PubMed35273725

2022

Di(2-ethylhexyl)phthalate impairs erythropoiesis via inducing Klotho expression and not via bioenergetic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Chang-Yi Tsai,Te-Ping Fang,Shuoh-Wen Chen,Hsiao-Wen Chen,Eric Chang-Yi Lin,Ting-An Lin,Der-Cherng Tarng,Yuan-I Chang

Molecular neurobiology 58:5954-5970 PubMed34435330

2021

Tacrolimus Decreases Cognitive Function by Impairing Hippocampal Synaptic Balance: a Possible Role of Klotho.

Applications

Unspecified application

Species

Unspecified reactive species

Yoo Jin Shin,Sun Woo Lim,Sheng Cui,Eun Jeong Ko,Byung Ha Chung,Hong Lim Kim,Tae Ryong Riew,Mun Yong Lee,Chul Woo Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Klotho antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (AB203576)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody (AB203576)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Secondary Antibodies

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Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (25)

Product promise