Anti-Klotho antibody [EPR6856] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] - BSA and Azide free (AB232366)

Immunohistochemical analysis of paraffin-embedded human kidney clear cell carcinoma tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] - BSA and Azide free (AB232366)

Immunohistochemical staining of paraffin embedded human kidney with purified ab181373 at a working dilution of 1 in 300. The secondary antibody used is a HRP polymer for rabbit IgG. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Klotho antibody [EPR6856] - BSA and Azide free (AB232366)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Klotho with unpurified ab181373 at 1/100 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Klotho antibody [EPR6856] - BSA and Azide free (AB232366)

The blots were produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane were blocked for an hour before being incubated with ab181373 (rabbit anti-Klotho antibody; diluted 5 ug/ml) for 24 hours at 4°C. Antibody binding was detected using infrared labelled goat anti-rabbit (green) antibody at 1 : 10,000 dilutions for 1 hour at room temperature before imaging.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181373).

All lanes:

Western blot - Anti-Klotho antibody [EPR6856] (<a href='/en-us/products/primary-antibodies/klotho-antibody-epr6856-ab181373'>ab181373</a>) at 5 µg/mL

Lane 1:

Wild Type (WT) Mouse Kidney Tissue Lysate at 20 µg

Lane 2:

Knock Out (KO) Mouse Kidney Tissue Lysate at 20 µg

Predicted band size: 116 kDa,38 kDa,84 kDa

Observed band size: 50 kDa,72 kDa

false

• WB

CiteAb

Western blot - Anti-Klotho antibody [EPR6856] - BSA and Azide free (AB232366)

Klotho western blot using anti-Klotho antibody [EPR6856] - BSA and Azide free ab232366. Publication image and figure legend from Chen, Z., Zhou, Q., et al., 2020, Int J Med Sci, PubMed 33162804.

ab232366 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab232366 please see the product overview.

Klotho deficiency may aggravate diabetes-induced renal dysfunction. (a)The protein levels of Klotho in the kidneys were detected by immunoblot. (b-e) Serum Klotho levels (Vehicle (injection of citrate buffer) =6, STZ (injection of 55 mg/kg/d STZ) =6) (b), serum glucose levels (c), Urine volume (d) and kidney weight (e). (f) Ratio of kidney weight to body weight in STZ-induced diabetic mice and normal mice. Comparison of serum creatinine (g) and Bun (h) between vehicle mice and STZ mice. (i)UACR (urine albumin-to-creatinine ratio) of STZ-induced diabetic mice and normal mice. (j) Comparison of mesangial matrix expansion and glycogen deposition in glomerulus of vehicle mice and STZ mice by HE staining and PAS staining, respectively. Scale bar : 20 μm. *p<0.05; **p<0.01; ***p<0.001. Veh, vehicle.

false