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AB277490

Anti-KLRF1 antibody [EPR23633-150] - BSA and Azide free

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Rabbit Recombinant Monoclonal KLRF1 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CLEC5C, ML, KLRF1, Killer cell lectin-like receptor subfamily F member 1, Lectin-like receptor F1, Activating coreceptor NKp80, C-type lectin domain family 5 member C

1 Images
Flow Cytometry - Anti-KLRF1 antibody [EPR23633-150] - BSA and Azide free (AB277490)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-KLRF1 antibody [EPR23633-150] - BSA and Azide free (AB277490)

This data was developed using ab256809, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labelling KLRF1 with ab256809 at 1/500 dilution (0.1ug) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG or ab256809. Then stained with anti-CD56 conjugated to BV421.

Gated on viable cells.

  • Unconjugated

    Anti-KLRF1 antibody [EPR23633-150]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-KLRF1 antibody [EPR23633-150]

  • 578 PE

    PE Anti-KLRF1 antibody [EPR23633-150]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23633-150

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab277490 is the carrier-free version of ab256809.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KLRF1 also known as CD314 or NKp80 is a receptor protein found primarily on natural killer (NK) cells with a significant mass of about 30 kDa. Researchers have identified its expression mainly in tissues rich in NK cells such as the spleen and peripheral blood. This receptor structurally belongs to the C-type lectin-like domain family and plays an important role in the immune response by binding to the CLEC2B ligand on target cells facilitating NK cell activation and cytotoxicity.
Biological function summary

In immune surveillance and homeostasis KLRF1 acts as an important activating receptor on natural killer cells. It does not form a complex but works independently to control cellular immune responses by recognizing stress-induced ligands on target cells. Upon interaction with its ligand KLRF1 triggers a cascade of intracellular signals that promote cytotoxic activity and cytokine secretion contributing to the elimination of virally infected or transformed cells.

Pathways

In the context of innate immune response signaling KLRF1 interacts with pathways involving NK cell-mediated cytotoxicity. KLRF1 engagement stimulates pathways similar to those activated by other receptors in the natural killer cell repertoire such as KLRK1 (NKG2D). These interactions play roles in maintaining balance within the immune system by modulating NK cell activity and ensuring efficient target cell elimination.

KLRF1 has implications in immune system-related conditions such as cancers and viral infections. Altered expression or function of KLRF1 may contribute to the escape of tumor cells from immune surveillance or decreased viral clearance. Associations with proteins like KLRD1 (CD94) in certain hematological malignancies suggest a role for KLRF1 in disease pathogenesis where NK cell dysfunction is present underlining its significance in developing therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Functions as an activating receptor involved in immunosurveillance upon binding to various ligands displayed at the surface of myeloid cells. Upon interaction with CLEC2B ligand, stimulates NK-cell cytotoxicity and cytokine production leading to the cytolysis of malignant CLEC2B-expressing myeloid cells. Actviation of the common cytotoxicity pathway involves SRC and SYK kinases (PubMed : 21149606).
See full target information KLRF1
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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