AB190870

Anti-KMT1B / SUV39H2 antibody [EPR18495]

RabMAb
Recombinant
KO Validated
20ul selling size
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(6 Publications)

Rabbit Recombinant Monoclonal KMT1B / SUV39H2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

View Alternative Names

KMT1B, SUV39H2, Histone-lysine N-methyltransferase SUV39H2, Histone H3-K9 methyltransferase 2, Lysine N-methyltransferase 1B, Suppressor of variegation 3-9 homolog 2, H3-K9-HMTase 2, Su(var)3-9 homolog 2

8 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear staining on germ cells of human testis is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

KMT1B / SUV39H2 was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab190870 at 1/50 dilution.

Western blot was performed from the immunoprecipitate using ab190870 at 1/1000 dilution.

VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : SH-SY5Y whole cell lysate 10μg (Input).

Lane 2 : ab190870 IP in SH-SY5Y whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab190870 in SH-SY5Y whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

Predicted band size: 47 kDa

false

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nucleuarstaining on germ cells of mouse testis is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Nuclear staining on germ cells of rat testis is observed.

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Blocking/Dilution buffer : 5% NFDM/TBST.

The full length SUV39H2 orthologues differ in size : human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).

All lanes:

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/10000 dilution

Lane 1:

HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa,54 kDa

true

Exposure time: 3min

Lab

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Lanes 1 - 4 : Merged signal (red and green). Green - ab190870 observed at 47 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab190870 was shown to recognize SUV39H2 in wild-type U2-OS cells as signal was lost at the expected MW in SUV39H2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SUV39H2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab190870 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution

Lane 1:

Wild-type U2-OS whole cell lysate at 20 µg

Lane 2:

SUV39H2 knockout U2-OS whole cell lysate at 20 µg

Lane 2:

Western blot - Human SUV39H2 knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-suv39h2-knockout-u-2-os-cell-line-ab261882'>ab261882</a>)

Lane 3:

HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

Lane 4:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Supplier Data

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Blocking/Dilution buffer : 5% NFDM/TBST.

The full length SUV39H2 orthologues differ in size : human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).

All lanes:

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/5000 dilution

Lane 1:

Human testis lysate at 10 µg

Lane 2:

Mouse testis lysate at 10 µg

Lane 3:

Rat testis lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa,54 kDa

true

Exposure time: 3min

Unknown

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Blocking/Dilution buffer : 5% NFDM/TBST

All lanes:

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution

Lane 1:

SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate. at 20 µg

Lane 2:

MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

Lane 3:

Human fetal kidney at 10 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Lane 3:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

true

Exposure time: 3min

Carrier free

Anti-KMT1B / SUV39H2 antibody [EPR18495] - BSA and Azide free
665 Alexa Fluor® 647

Alexa Fluor® 647 Anti-KMT1B / SUV39H2 antibody [EPR18495]
660 APC

APC Anti-KMT1B / SUV39H2 antibody [EPR18495]
617 Alexa Fluor® 594

Alexa Fluor® 594 Anti-KMT1B / SUV39H2 antibody [EPR18495]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-KMT1B / SUV39H2 antibody [EPR18495]
578 PE

PE Anti-KMT1B / SUV39H2 antibody [EPR18495]
565 Alexa Fluor® 555

Alexa Fluor® 555 Anti-KMT1B / SUV39H2 antibody [EPR18495]
775 Alexa Fluor® 750

Alexa Fluor® 750 Anti-KMT1B / SUV39H2 antibody [EPR18495]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18495

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT1B also known as SUV39H2 is a lysine-specific histone methyltransferase enzyme located on chromosome 10q22.2. It catalyzes the methylation of histone H3 at lysine 9 (H3K9) which condenses chromatin into a more transcriptionally inactive state. KMT1B is approximately 50 kDa in mass and shows highest expression in testis and fetal tissues. It modifies chromatin structure to regulate gene expression patterns affecting cellular processes.

Biological function summary

This protein influences epigenetic constructs by being part of the transcriptional repressor complex. It is involved in gene silencing through the formation of heterochromatin an essential process for maintaining genome stability. KMT1B shares homology with SUV39H1 another histone methyltransferase and both cooperate in establishing heterochromatin markers.

Pathways

KMT1B participates in the transcriptional regulation pathway and intersects with pathways governing cell cycle control. It contributes to the E2F transcriptional repression pathway by interacting with retinoblastoma protein (pRB) which controls cell proliferation. This connection to pRB positions KMT1B as an influential factor in cell cycle checkpoints and progression.

KMT1B mutations have associations with neurodevelopmental disorders and some cancers. Its dysregulation can lead to anomalies in chromatin compaction affecting neurological development and potentially promoting oncogenesis. In cancer changes in KMT1B expression relate to abnormal cell growth often linked with parallel alterations in related enzymes like SUV39H1.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.

See full target information SUV39H2

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:957 PubMed35177609

2022

RIF1-ASF1-mediated high-order chromatin structure safeguards genome integrity.

Applications

Unspecified application

Species

Unspecified reactive species

Sumin Feng,Sai Ma,Kejiao Li,Shengxian Gao,Shaokai Ning,Jinfeng Shang,Ruiyuan Guo,Yingying Chen,Britny Blumenfeld,Itamar Simon,Qing Li,Rong Guo,Dongyi Xu

Eye and vision (London, England) 9:4 PubMed35101125

2022

SUV39H1 regulates corneal epithelial wound healing via H3K9me3-mediated repression of p27.

Applications

Unspecified application

Species

Unspecified reactive species

Shuai Yang,Weiwei Chen,Shanshan Jin,Guangying Luo,Xia Jing,Qi Liu,Peter S Reinach,Jia Qu,Dongsheng Yan

Cancer cell international 21:2 PubMed33397384

2021

Histone methyltransferase SUV39H2 regulates LSD1-dependent CDH1 expression and promotes epithelial mesenchymal transition of osteosarcoma.

Applications

Unspecified application

Species

Unspecified reactive species

Yingying Miao,Guifeng Liu,Lin Liu

Adipocyte 9:401-414 PubMed32698678

2020

The histone methyltransferase Suv39h regulates 3T3-L1 adipogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jia Jing,Fenfen Li,Lin Zha,Xiaosong Yang,Rui Wu,Shirong Wang,Bingzhong Xue,Hang Shi

Neoplasia (New York, N.Y.) 22:376-389 PubMed32629178

2020

BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases.

Applications

Unspecified application

Species

Unspecified reactive species

Florina Grigore,Hana Yang,Nicholas D Hanson,Matthew W VanBrocklin,Aaron L Sarver,James P Robinson

Cancer letters 422:56-69 PubMed29458143

2018

SUV39H2 promotes colorectal cancer proliferation and metastasis via tri-methylation of the SLIT1 promoter.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Wendi Shuai,Jiangxue Wu,Shuai Chen,Ranyi Liu,Zhihua Ye,Chunmei Kuang,Xiang Fu,Gaoyuan Wang,Yingchang Li,Qihua Peng,Wei Shi,Yizhuo Li,Qianghua Zhou,Wenlin Huang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT1B / SUV39H2 antibody [EPR18495]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870)

Related conjugates and formulations (8)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise