Anti-KMT1B / SUV39H2 antibody [EPR18495] KO tested (ab190870)

Rabbit Recombinant Monoclonal KMT1B / SUV39H2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-P
Human	Tested	Tested	Tested
Mouse	Expected	Tested	Tested
Rat	Expected	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/50	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/500	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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2 products for Alternative Product

Target data

See full target information SUV39H2

Function

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.

Alternative names

KMT1B, SUV39H2, Histone-lysine N-methyltransferase SUV39H2, Histone H3-K9 methyltransferase 2, Lysine N-methyltransferase 1B, Suppressor of variegation 3-9 homolog 2, H3-K9-HMTase 2, Su(var)3-9 homolog 2

Recommended products

Rabbit Recombinant Monoclonal KMT1B / SUV39H2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR18495
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

KMT1B also known as SUV39H2 is a lysine-specific histone methyltransferase enzyme located on chromosome 10q22.2. It catalyzes the methylation of histone H3 at lysine 9 (H3K9) which condenses chromatin into a more transcriptionally inactive state. KMT1B is approximately 50 kDa in mass and shows highest expression in testis and fetal tissues. It modifies chromatin structure to regulate gene expression patterns affecting cellular processes.

Biological function summary

This protein influences epigenetic constructs by being part of the transcriptional repressor complex. It is involved in gene silencing through the formation of heterochromatin an essential process for maintaining genome stability. KMT1B shares homology with SUV39H1 another histone methyltransferase and both cooperate in establishing heterochromatin markers.

Pathways

KMT1B participates in the transcriptional regulation pathway and intersects with pathways governing cell cycle control. It contributes to the E2F transcriptional repression pathway by interacting with retinoblastoma protein (pRB) which controls cell proliferation. This connection to pRB positions KMT1B as an influential factor in cell cycle checkpoints and progression.

Associated diseases and disorders

KMT1B mutations have associations with neurodevelopmental disorders and some cancers. Its dysregulation can lead to anomalies in chromatin compaction affecting neurological development and potentially promoting oncogenesis. In cancer changes in KMT1B expression relate to abnormal cell growth often linked with parallel alterations in related enzymes like SUV39H1.

Product promise

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8 product images

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Lanes 1 - 4: Merged signal (red and green). Green - ab190870 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.
ab190870 was shown to recognize SUV39H2 in wild-type U2-OS cells as signal was lost at the expected MW in SUV39H2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SUV39H2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab190870 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution
Lane 1: Wild-type U2-OS whole cell lysate at 20 µg
Lane 2: SUV39H2 knockout U2-OS whole cell lysate at 20 µg
Lane 2: Western blot - Human SUV39H2 knockout U-2 OS cell line (Human SUV39H2 knockout U-2 OS cell line ab261882)
Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg
Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Blocking/Dilution buffer: 5% NFDM/TBST
All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution
Lane 1: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate. at 20 µg
Lane 2: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
Lane 3: Human fetal kidney at 10 µg
Secondary
Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Lane 3: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 3min
Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Blocking/Dilution buffer: 5% NFDM/TBST.
The full length SUV39H2 orthologues differ in size: human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).
All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/10000 dilution
Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg
Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa, 54 kDa
Exposure time: 3min
Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Blocking/Dilution buffer: 5% NFDM/TBST.
The full length SUV39H2 orthologues differ in size: human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).
All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/5000 dilution
Lane 1: Human testis lysate at 10 µg
Lane 2: Mouse testis lysate at 10 µg
Lane 3: Rat testis lysate at 10 µg
Secondary
All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 47 kDa
Observed band size: 47 kDa, 54 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nuclear staining on germ cells of human testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
KMT1B / SUV39H2 was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab190870 at 1/50 dilution.
Western blot was performed from the immunoprecipitate using ab190870 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: SH-SY5Y whole cell lysate 10μg (Input).
Lane 2: ab190870 IP in SH-SY5Y whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab190870 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
All lanes: Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Predicted band size: 47 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nucleuarstaining on germ cells of mouse testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)
Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Nuclear staining on germ cells of rat testis is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.