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Rabbit Recombinant Monoclonal KMT1B / SUV39H2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

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Images

Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870), expandable thumbnail
  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870), expandable thumbnail
  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870), expandable thumbnail
  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (AB190870), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-P
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Tested
Rat
Expected
Tested
Tested

Tested
Tested

Species

Human

Dilution info

1/50

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/500

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

2 products for Alternative Product

Target data

Function

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 using monomethylated H3 'Lys-9' as substrate. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin at pericentric and telomere regions. H3 'Lys-9' trimethylation is also required to direct DNA methylation at pericentric repeats. SUV39H1 is targeted to histone H3 via its interaction with RB1 and is involved in many processes, such as cell cycle regulation, transcriptional repression and regulation of telomere length. May participate in regulation of higher-order chromatin organization during spermatogenesis. Recruited by the large PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1, contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation.

Alternative names

Histone-lysine N-methyltransferase SUV39H2, Histone H3-K9 methyltransferase 2, Lysine N-methyltransferase 1B, Suppressor of variegation 3-9 homolog 2, H3-K9-HMTase 2, Su(var)3-9 homolog 2, SUV39H2, KMT1B

Recommended products

Rabbit Recombinant Monoclonal KMT1B / SUV39H2 antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 6 publications.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR18495

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Lanes 1 - 4: Merged signal (red and green). Green - ab190870 observed at 47 kDa. Red - loading control, Anti-GAPDH antibody [6C5] - Loading Control ab8245, observed at 37 kDa.

    ab190870 was shown to recognize SUV39H2 in wild-type U2-OS cells as signal was lost at the expected MW in SUV39H2 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and SUV39H2 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% Milk. ab190870 and Anti-GAPDH antibody [6C5] - Loading Control ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution

    Lane 1: Wild-type U2-OS whole cell lysate at 20 µg

    Lane 2: SUV39H2 knockout U2-OS whole cell lysate at 20 µg

    Lane 3: HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate at 20 µg

    Lane 4: HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Blocking/Dilution buffer: 5% NFDM/TBST

    All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/1000 dilution

    Lane 1: SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate. at 20 µg

    Lane 2: MOLT-4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg

    Lane 3: Human fetal kidney at 10 µg

    Secondary

    Lanes 1 - 2: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Lane 3: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

    Exposure time: 3min

  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The full length SUV39H2 orthologues differ in size: human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).

    All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/10000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate at 20 µg

    Lane 2: HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg

    Lane 3: NIH/3T3 (Mouse embryonic fibroblast cell line) cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa, 54 kDa

    Exposure time: 3min

  • Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Blocking/Dilution buffer: 5% NFDM/TBST.

    The full length SUV39H2 orthologues differ in size: human 410aa (UniProt Q9H5I1), mouse 477aa (UniProt Q9EQQ0) and rat 481aa (UniProt D3ZIH5).

    All lanes: Western blot - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870) at 1/5000 dilution

    Lane 1: Human testis lysate at 10 µg

    Lane 2: Mouse testis lysate at 10 µg

    Lane 3: Rat testis lysate at 10 µg

    Secondary

    All lanes: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 47 kDa

    Observed band size: 47 kDa, 54 kDa

    Exposure time: 3min

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Nuclear staining on germ cells of human testis is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    KMT1B / SUV39H2 was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate with ab190870 at 1/50 dilution.

    Western blot was performed from the immunoprecipitate using ab190870 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.

    Lane 1: SH-SY5Y whole cell lysate 10μg (Input).

    Lane 2: ab190870 IP in SH-SY5Y whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab190870 in SH-SY5Y whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    All lanes: Immunoprecipitation - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Predicted band size: 47 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Nucleuarstaining on germ cells of mouse testis is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1B / SUV39H2 antibody [EPR18495] (ab190870)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue labeling KMT1B / SUV39H2 with ab190870 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Nuclear staining on germ cells of rat testis is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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