AB107225

Anti-KMT1E / SETDB1 antibody [5H6A12]

Name: Anti-KMT1E / SETDB1 antibody [5H6A12] Mouse monoclonal (ab107225) | Abcam
Brand: Abcam Limited
SKU: AB107225
Price: 410 USD
Availability: InStock
Rating: 3 (2 reviews)

(2 Reviews)

(16 Publications)

Mouse Monoclonal KMT1E / SETDB1 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, African green monkey, Mouse samples. Cited in 16 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.

View Alternative Names

ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).

Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

Flow Cyt

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Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

All lanes:

Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution

Lane 1:

MCF-7

Lane 2:

T47D

Lane 3:

HEK293

Lane 4:

JURKAT

Lane 5:

NIH/3T3

Lane 6:

Lane 7:

RAW264.7

Lane 8:

COS-7

Predicted band size: 143 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5H6A12

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human, African green monkey

Applications

WB, ICC/IF, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information.

Q15047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

This product was changed from ascites to supernatant. Lot no's high than GR223392-13 are from Tissue Culture Supernatant

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.

Biological function summary

The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.

Pathways

Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.

SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed : 12869583, PubMed : 27237050, PubMed : 39096901). Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed : 14536086, PubMed : 27732843). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed : 14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610).

See full target information SETDB1

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Nucleic acids research 53: PubMed40521666

2025

Nat10 maintains the homeostasis of pluripotent and 2-cell-like states in mouse embryonic stem cells through mRNA cytidine acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Guofeng Feng,Guoxing Yin,Yusheng Liu,Chang Liu,Jie Li,Jiangtao Lu,Yongqin Yu,Ziyi Jin,Yiwei Wu,Yanbin Yue,Xiufang Gao,Jiaqiang Wang,Falong Lu,Lin Liu

Journal of inflammation research 18:5931-5950 PubMed40357375

2025

Mechanisms of Acetate in Alleviating SETDB1-Linked Neuroinflammation and Cognitive Impairment in a Mouse Model of OSA.

Applications

Unspecified application

Species

Unspecified reactive species

Zhan Zhao,Li Xiang,Jau-Shyong Hong,Yubao Wang,Jing Feng

Journal of cardiothoracic surgery 20:51 PubMed39780196

2025

SNX30 inhibits lung adenocarcinoma cell proliferation and induces cell ferroptosis through regulating SETDB1.

Applications

Unspecified application

Species

Unspecified reactive species

Xinjie Fan,Qichu Zhu,Chengzhuo Du,Jinlai Chen,Yingming Su

Nucleic acids research 52:13690-13705 PubMed39540436

2024

SETDB1 activity is globally directed by H3K14 acetylation via its Triple Tudor Domain.

Applications

Unspecified application

Species

Unspecified reactive species

Thyagarajan T Chandrasekaran,Michel Choudalakis,Alexander Bröhm,Sara Weirich,Alexandra G Kouroukli,Ole Ammerpohl,Philipp Rathert,Pavel Bashtrykov,Albert Jeltsch

Science advances 10:eadj8042 PubMed38691608

2024

SETDB1 modulates the TGFβ response in Duchenne muscular dystrophy myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Alice Granados,Maeva Zamperoni,Roberta Rapone,Maryline Moulin,Ekaterina Boyarchuk,Costas Bouyioukos,Laurence Del Maestro,Véronique Joliot,Elisa Negroni,Myriame Mohamed,Sandra Piquet,Anne Bigot,Fabien Le Grand,Sonia Albini,Slimane Ait-Si-Ali

iScience 26:107386 PubMed37559904

2023

The cytoplasmic fraction of the histone lysine methyltransferase Setdb1 is essential for embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Roberta Rapone,Laurence Del Maestro,Costas Bouyioukos,Sonia Albini,Paola Cruz-Tapias,Véronique Joliot,Bertrand Cosson,Slimane Ait-Si-Ali

Molecular cell 83:1075-1092.e9 PubMed36868228

2023

DAXX adds a de novo H3.3K9me3 deposition pathway to the histone chaperone network.

Applications

Unspecified application

Species

Unspecified reactive species

Massimo Carraro,Ivo A Hendriks,Colin M Hammond,Victor Solis-Mezarino,Moritz Völker-Albert,Jonas D Elsborg,Melanie B Weisser,Christos Spanos,Guillermo Montoya,Juri Rappsilber,Axel Imhof,Michael L Nielsen,Anja Groth

Nucleic acids research 50:4389-4413 PubMed35474385

2022

SETDB1 fuels the lung cancer phenotype by modulating epigenome, 3D genome organization and chromatin mechanical properties.

Applications

Unspecified application

Species

Unspecified reactive species

Vlada V Zakharova,Mikhail D Magnitov,Laurence Del Maestro,Sergey V Ulianov,Alexandros Glentis,Burhan Uyanik,Alice Williart,Anna Karpukhina,Oleg Demidov,Veronique Joliot,Yegor S Vassetzky,René-Marc Mège,Matthieu Piel,Sergey V Razin,Slimane Ait-Si-Ali

Bioengineered 13:5737-5755 PubMed35184652

2022

Enhancer of zeste homolog 2 promotes hepatocellular cancer progression and chemoresistance by enhancing protein kinase B activation through microRNA-381-mediated SET domain bifurcated 1.

Applications

Unspecified application

Species

Unspecified reactive species

Jingyang Zhou,Jinhui Che,Lu Xu,Weizhong Yang,Yunmei Li,Wuyuan Zhou,Shubing Zou

Nature communications 12:3034 PubMed34031396

2021

MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Iris Müller,Ann Sophie Moroni,Daria Shlyueva,Sudeep Sahadevan,Erwin M Schoof,Aliaksandra Radzisheuskaya,Jonas W Højfeldt,Tülin Tatar,Richard P Koche,Chang Huang,Kristian Helin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT1E / SETDB1 antibody [5H6A12]

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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