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Mouse Monoclonal KMT1E / SETDB1 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, African green monkey, Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.


Images

Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225), expandable thumbnail
  • Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (AB107225), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information. Database link Q15047

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWBICC/IF
Human
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
African green monkey
Expected
Tested
Expected

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
African green monkey, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
African green monkey
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Species
Mouse
Dilution info
1/500.00000 - 1/2000.00000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/200.00000 - 1/1000.00000
Notes

-

Expected
Expected

Species
African green monkey, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed:12869583, PubMed:27237050, PubMed:39096901). Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed:14536086, PubMed:27732843). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed:14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).

Alternative names

ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4

Recommended products

Mouse Monoclonal KMT1E / SETDB1 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human, African green monkey, Mouse samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information. Database link Q15047
Clone number
5H6A12
Purification technique
Affinity purification Protein G
Concentration
Loading...
Purification notes

Purified from tissue culture supernatant.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to supernatant. Lot no's high than GR223392-13 are from Tissue Culture Supernatant

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Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.

Biological function summary

The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.

Pathways

Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.

Associated diseases and disorders

SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.

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3 product images

  • Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225), expandable thumbnail

    Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)

    All lanes: Western blot - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225) at 1/500 dilution

    Lane 1: MCF-7

    Lane 2: T47D

    Lane 3: HEK293

    Lane 4: JURKAT

    Lane 5: NIH/3T3

    Lane 6: F9

    Lane 7: RAW264.7

    Lane 8: COS-7

    Predicted band size: 143 kDa

  • Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225), expandable thumbnail

    Flow Cytometry - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)

    Overlay histogram showing Ramos cells stained with ab107225 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab107225, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Ramos cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [5H6A12] (ab107225)

    Immunofluoresence detection of SETDB1 in LOVO cells using ab107225 (green). Actin fillaments have been labeled with Alexa Fluor-555 phalloidin (red).

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Product protocols

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