Rabbit Polyclonal KMT1E / SETDB1 antibody. Suitable for WB and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human SETDB1 aa 1100-1200.
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- Molecular cell 82:816-832.e122022SETDB1/NSD-dependent H3K9me3/H3K36me3 dual heterochromatin maintains gene expression profiles by bookmarking poised enhancers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAmandine Barral,Gabrielle Pozo,Lucas Ducrot,Giorgio L Papadopoulos,Sandrine Sauzet,Andrew J Oldfield,Giacomo Cavalli,Jérôme DéjardinPubMed 35081363
- The Journal of experimental medicine 219:2021WEE1 inhibition induces anti-tumor immunity by activating ERV and the dsRNA pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEnsong Guo,Rourou Xiao,Yifan Wu,Funian Lu,Chen Liu,Bin Yang,Xi Li,Yu Fu,Zizhuo Wang,Yuan Li,Yuhan Huang,Fuxia Li,Xue Wu,Lixin You,Tianyu Qin,Yiling Lu,Xiaoyuan Huang,Ding Ma,Gordon B Mills,Chaoyang Sun,Gang ChenPubMed 34825915
- ACS chemical biology 16:1721-17362021A Peptidomimetic Ligand Targeting the Chromodomain of MPP8 Reveals HRP2's Association with the HUSH Complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJarod M Waybright,Sarah E Clinkscales,Kimberly D Barnash,Gabrielle R Budziszewski,Justin M Rectenwald,Anna M Chiarella,Jacqueline L Norris-Drouin,Stephanie H Cholensky,Kenneth H Pearce,Laura E Herring,Robert K McGinty,Nathaniel A Hathaway,Lindsey I JamesPubMed 34415726
- PLoS pathogens 16:e10088342020Genome-wide CRISPR knockout screen identifies ZNF304 as a silencer of HIV transcription that promotes viral latency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Krasnopolsky et. al.PubMed 32956422
- Journal of virology 94:2020An Alternatively Spliced Sirtuin 2 Isoform 5 Inhibits Hepatitis B Virus Replication from cccDNA by Repressing Epigenetic Modifications Made by Histone Lysine Methyltransferases.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZahra Zahid Piracha et. al.PubMed 32493816
- Cell 181:800-817.e222020Heterochromatin-Driven Nuclear Softening Protects the Genome against Mechanical Stress-Induced Damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichele M Nava et. al.PubMed 32302590
- Epigenetics & chromatin 13:62020JMJD1B, a novel player in histone H3 and H4 processing to ensure genome stability.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFrancisco Saavedra et. al.PubMed 32070414
- Molecules and cells 42:884-8922019Increased Expression of FosB through Reactive Oxygen Species Accumulation Functions as Pro-Apoptotic Protein in Piperlongumine Treated MCF7 Breast Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin-Ah Park,Han-Heom Na,Hyeon-Ok Jin,Keun-Cheol KimPubMed 31735020
- Nucleic acids research 47:8548-85622019Cockayne syndrome group B deficiency reduces H3K9me3 chromatin remodeler SETDB1 and exacerbates cellular aging.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJong-Hyuk Lee,Tyler G Demarest,Mansi Babbar,Edward W Kim,Mustafa N Okur,Supriyo De,Deborah L Croteau,Vilhelm A BohrPubMed 31276581
- International journal of cancer 145:3462-34772019Histone methyltransferase SETDB1 contributes to melanoma tumorigenesis and serves as a new potential therapeutic target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElias Orouji,Aniello Federico,Lionel Larribère,Daniel Novak,Daniel B Lipka,Yassen Assenov,Sachindra Sachindra,Laura Hüser,Karol Granados,Christoffer Gebhardt,Christoph Plass,Viktor Umansky,Jochen UtikalPubMed 31131878
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human SETDB1 aa 1100-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q15047
Reactivity data
Select an application| WB | |
|---|---|
| Human | Tested |
| Mouse | Predicted |
| Chimpanzee | Predicted |
| Gorilla | Predicted |
| Orangutan | Predicted |
| Rhesus monkey | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Mouse | Dilution info - | Notes - |
Associated Products
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3 products for Alternative Product
Target data
Function
Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed:12869583, PubMed:27237050, PubMed:39096901). Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed:14536086, PubMed:27732843). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed:14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed:24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed:24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (By similarity). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed:24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed:27029610).
Alternative names
ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4
Recommended products
Rabbit Polyclonal KMT1E / SETDB1 antibody. Suitable for WB and reacts with Human samples. Cited in 28 publications. Immunogen corresponding to Synthetic Peptide within Human SETDB1 aa 1100-1200.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.1% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human SETDB1 aa 1100-1200. The exact immunogen used to generate this antibody is proprietary information. Database link Q15047
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified using the peptide immobilized on solid support.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.
- Biological function summary
The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.
- Pathways
Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.
- Associated diseases and disorders
SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.
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Tested
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Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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1 product image
Western blot - Anti-KMT1E / SETDB1 antibody (ab12317)
All lanes: Western blot - Anti-KMT1E / SETDB1 antibody (ab12317) at 1 µg/mL
Lane 1: HeLa whole cell lysate at 50 µg
Lane 2: HEK293T whole cell lysate at 50 µg
Predicted band size: 143 kDa
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