AB228575

Anti-KMT1E / SETDB1 antibody

Name: Anti-KMT1E / SETDB1 antibody (ab228575) | Abcam
Brand: Abcam Limited
SKU: AB228575
Price: 220 USD
Availability: InStock

Be the first to review this product! Submit a review

(3 Publications)

Rabbit Polyclonal KMT1E / SETDB1 antibody. Suitable for IP, ChIP, WB, ICC/IF, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.

View Alternative Names

ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody (AB228575)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody (AB228575)

Immunofluorescence analysis of HeLa cells fixed in 4% paraformaldehyde at RT for 15 min labelling SETDB1 protein at nucleus using ab228575 at a 1/500 dilution (Green).
alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody at a 1/1000 dilution (Red). Scale bar = 10 µm

Supplier Data

Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (AB228575)

KMT1E / SETDB1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract using 4 μg of ab228575. Western blot was performed from the immunoprecipitate using ab228575 at 1/1000 dilution.

Lane 1 : Control IgG IP in HeLa nuclear extract.

Lane 2 : ab228575 IP in HeLa nuclear extract.

5% SDS-PAGE

All lanes:

Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (ab228575)

Predicted band size: 143 kDa

false

ChIP - Anti-KMT1E / SETDB1 antibody (AB228575)

ChIP

Supplier Data

ChIP - Anti-KMT1E / SETDB1 antibody (AB228575)

Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228575. The precipitated DNA was detected by PCR with primer set targeting to p53P2.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1E / SETDB1 antibody (AB228575)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1E / SETDB1 antibody (AB228575)

Immunohistochemical analysis of paraffin-embedded mouse testis tissue stained for SETDB1 protein at nucleus with ab228575 at a 1/500 dilution.

Antigen retrieval : Citrate buffer, pH 6.0, 15 min.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IHC-P, ChIP, WB, IP, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information.

Q15047

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "guaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.

Biological function summary

The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.

Pathways

Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.

SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed : 14536086). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed : 14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins (PubMed : 24623306). Required for HUSH-mediated heterochromatin formation and gene silencing (PubMed : 27732843). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (PubMed : 37938770). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610). Also catalyzes mono- and dimethylation of 'Lys-9' of free histone H3 (H3K9me1 and H3K9me2) during translation in the cytoplasm, which is then transported to the nucleus and incorporated into nucleosomes (PubMed : 26405197).. Isoform 2. Lacks all domains required for histone methyltransferase activity.

See full target information SETDB1

style

left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Cancer cell international 25:290 PubMed40722171

2025

mA-modified CTC-297N7.9 inhibits hepatocellular carcinoma metastasis via epigenetically downregulating CCL2 and CD47.

Applications

Unspecified application

Species

Unspecified reactive species

Huamei Wei,Changhong Pu,Min Zeng,Rongzhou Lu,Yunyu Wei,Yanyan Huang,Zheng Huang,Lizheng Huang,Zuoming Xu,Jianchu Wang,Rihai Ma,Jian Pu

Thoracic cancer 16:e15485 PubMed39552193

2024

USP8-mediated PTK7 promotes PIK3CB-related pathway to accelerate the malignant progression of non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wencui Kong,Xuegang Feng,Zongyang Yu,Xingfeng Qi,Zhongquan Zhao

Journal of immunology research 2022:4012920 PubMed35497876

2022

Histone Methyltransferase SETDB1 Promotes Immune Evasion in Colorectal Cancer FOSB-Mediated Downregulation of MicroRNA-22 through BATF3/PD-L1 Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jiale Tian,Weiwei Wang,Jichao Zhu,Yun Zhuang,Chunrun Qi,Zhengxin Cai,Wenhui Yan,Wenying Lu,Anquan Shang

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT1E / SETDB1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody (AB228575)

Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (AB228575)

ChIP - Anti-KMT1E / SETDB1 antibody (AB228575)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT1E / SETDB1 antibody (AB228575)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise