AB228984

Anti-KMT1E / SETDB1 antibody

Name: Anti-KMT1E / SETDB1 antibody (ab228984) | Abcam
Brand: Abcam Limited
SKU: AB228984

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(1 Publication)

Rabbit Polyclonal KMT1E / SETDB1 antibody. Suitable for IP, ChIP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.

View Alternative Names

ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4

3 Images

Supplier Data

Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (AB228984)

ab228984 immunoprecipitates SETDB1 protein in IP experiments.
IP samples : HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear extract.

Lane 1 : Control IP with 4 μg of preimmune Rabbit IgG.
Lane 2 : Immunoprecipitation of SETDB1 protein by 4 μg ab228984.

The immunoprecipitated SETDB1 protein was detected by ab228984 at 1/1000 dilution.

5 % SDS-PAGE

All lanes:

Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (ab228984)

Predicted band size: 143 kDa

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ChIP - Anti-KMT1E / SETDB1 antibody (AB228984)

ChIP

Supplier Data

ChIP - Anti-KMT1E / SETDB1 antibody (AB228984)

Cross-linked ChIP was performed with HeLa (human epithelial cell line from cervix adenocarcinoma) chromatin extract and 5 μg of either control rabbit IgG or ab228984. The precipitated DNA was detected by PCR with a primer set targeting to P53P2.

Supplier Data

Western blot - Anti-KMT1E / SETDB1 antibody (AB228984)

Samples were separated by 5% SDS-PAGE.

All lanes:

Western blot - Anti-KMT1E / SETDB1 antibody (ab228984) at 1/2000 dilution

All lanes:

U87-MG Whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 143 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ChIP

applications

Immunogen

Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information.

Q15047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "predicted", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.

Biological function summary

The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.

Pathways

Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.

SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed : 14536086). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed : 14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins (PubMed : 24623306). Required for HUSH-mediated heterochromatin formation and gene silencing (PubMed : 27732843). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (PubMed : 37938770). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610). Also catalyzes mono- and dimethylation of 'Lys-9' of free histone H3 (H3K9me1 and H3K9me2) during translation in the cytoplasm, which is then transported to the nucleus and incorporated into nucleosomes (PubMed : 26405197).. Isoform 2. Lacks all domains required for histone methyltransferase activity.

See full target information SETDB1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112177 PubMed40224025

2025

CCL7 promotes macrophage polarization and synovitis to exacerbate rheumatoid arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Chen,Shuo Shi,Xiaojia Li,Feng Gao,Xu Zhu,Ru Feng,Ke Hu,Yicheng Li,Shuiyuan Chen,Rongkai Zhang,Xiaoshuai Wang,Changhai Ding,Gang Liu,Tianyu Chen,Wenquan Liang

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Product promise

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Anti-KMT1E / SETDB1 antibody

Immunoprecipitation - Anti-KMT1E / SETDB1 antibody (AB228984)

ChIP - Anti-KMT1E / SETDB1 antibody (AB228984)

Western blot - Anti-KMT1E / SETDB1 antibody (AB228984)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise