Anti-KMT1E/SETDB1 antibody [EPR24218-43]

• IP

Supplier Data

Immunoprecipitation - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (AB300573)

KMT1E / SETDB1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 ug with ab300573 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300573 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Lysate was freshly made and used for immunoprecipitation immediately to minimize protein degradation.

The band beneath the target band (100 kDa) is likely to be degraded target fragments.

All lanes:

Immunoprecipitation - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (ab300573) at 1/1000 dilution

Lane 1:

K562 whole cell lysate at 10 µg

Lane 2:

ab300573 at 1/30 IP in K562 whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab300573 in K562 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 128s

• WB

Supplier Data

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (AB300573)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the lower MW band at approximately 70kDa is unknown.

All lanes:

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (ab300573) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (AB300573)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (ab300573) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (AB300573)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

All lanes:

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (ab300573) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate

Lane 2:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 48s