Anti-KMT1E/SETDB1 antibody [EPR24218-43] (BSA and Azide free)

• IP

Supplier Data

Immunoprecipitation - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (BSA and Azide free) (AB300574)

This data was developed using ab300573, the same antibody clone in a different buffer formulation.

KMT1E / SETDB1 was immunoprecipitated from 0.35 mg K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate 10 ug with ab300573 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab300573 at 1/1000 dilution.

VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Lysate was freshly made and used for immunoprecipitation immediately to minimize protein degradation.

The band beneath the target band (100 kDa) is likely to be degraded target fragments.

All lanes:

Immunoprecipitation - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (<a href='/en-us/products/primary-antibodies/kmt1e-setdb1-antibody-epr24218-43-ab300573'>ab300573</a>) at 1/1000 dilution

Lane 1:

K562 whole cell lysate at 10 µg

Lane 2:

<a href='/en-us/products/primary-antibodies/kmt1e-setdb1-antibody-epr24218-43-ab300573'>ab300573</a> at 1/30 IP in K562 whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/en-us/products/primary-antibodies/kmt1e-setdb1-antibody-epr24218-43-ab300573'>ab300573</a> in K562 whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

false

Exposure time: 128s

• WB

Supplier Data

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (BSA and Azide free) (AB300574)

This data was developed using ab300573, the same antibody clone in a different buffer formulation. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. The identity of the lower MW band at approximately 70kDa is unknown. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (<a href='/en-us/products/primary-antibodies/kmt1e-setdb1-antibody-epr24218-43-ab300573'>ab300573</a>) at 1/1000 dilution

All lanes:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (BSA and Azide free) (AB300574)

This data was developed using ab300573, the same antibody clone in a different buffer formulation. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (<a href='/en-us/products/primary-antibodies/kmt1e-setdb1-antibody-epr24218-43-ab300573'>ab300573</a>) at 1/1000 dilution

All lanes:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 48s

• WB

Supplier Data

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (BSA and Azide free) (AB300574)

This data was developed using ab300573, the same antibody clone in a different buffer formulation. Lysates were freshly made and used for Western blotting immediately to minimize protein degradation. Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-KMT1E/SETDB1 antibody [EPR24218-43] (<a href='/en-us/products/primary-antibodies/kmt1e-setdb1-antibody-epr24218-43-ab300573'>ab300573</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell), whole cell lysate

Lane 2:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa

false

Exposure time: 48s