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AB313862

Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free

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Rabbit Recombinant Monoclonal KMT1E / SETDB1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Recombinant Fragment Protein within Human SETDB1.

View Alternative Names

ESET, KIAA0067, KMT1E, SETDB1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, Histone H3-K9 methyltransferase 4, Lysine N-methyltransferase 1E, SET domain bifurcated 1, H3-K9-HMTase 4, Eset, Kiaa0067, Setdb1, Histone-lysine N-methyltransferase SETDB1, ERG-associated protein with SET domain, SET domain bifurcated 1, ESET

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (AB313862)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (AB313862)

Immunofluorescent analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells fixed in 4% paraformaldehyde at room temperature for 15 minutes labeling KMT1E / SETDB1 at the cytoplasm and nucleus with ab313862 at 1/500 dilution (green). Red : alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1/1000. Blue : Fluoroshield with DAPI.

Western blot - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (AB313862)
  • WB

Supplier Data

Western blot - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (AB313862)

All lanes:

Western blot - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (ab313862) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 2:

HeLa nuclear extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 143 kDa

false

Western blot - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (AB313862)
  • WB

Supplier Data

Western blot - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (AB313862)

All lanes:

Western blot - Anti-KMT1E / SETDB1 antibody [HL1645] - BSA and Azide free (ab313862) at 1/5000 dilution

Lane 1:

Mouse testis tissue extract at 50 µg

Lane 2:

Rat testis tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 145 kDa,146 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1645

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse, Rat

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human SETDB1. The exact immunogen used to generate this antibody is proprietary information.

Q15047

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT1E also known as SETDB1 is a lysine methyltransferase with a role in epigenetic regulation. This enzyme has a molecular weight of approximately 143 kDa. It actively methylates histone H3 at lysine 9 (H3K9) a modification associated with transcriptional repression. SETDB1 contains a SET domain responsible for its methyltransferase activity. Expressed mainly in the nucleus this enzyme plays a critical role in controlling gene expression across various tissues including the brain spleen and testis.
Biological function summary

The methyltransferase SETDB1 impacts chromatin structure and gene silencing. It operates as a part of the histone methylation complex collaborating with co-factors like ATF7IP and MBD1 to achieve highly specific gene repression. By catalyzing trimethylation of H3K9 it creates a binding site for HP1 proteins facilitating the formation of heterochromatin. This action is essential for processes such as X-chromosome inactivation and the repression of transposable elements.

Pathways

Regulation through SETDB1 influences key biological processes. This enzyme is integral to the PRC2 (Polycomb Repressive Complex 2) pathway maintaining transcriptional silencing during development. Furthermore SETDB1 works alongside SUV39H1 in dynamic change of heterochromatin states regulating genes involved in cell cycle and differentiation. Such pathways highlight its significant interactions with proteins like EZH2 and EED which assist in maintaining stable gene silencing.

SETDB1 shows a connection with cancer particularly in melanoma and lung cancer. Overexpression or dysregulation of SETDB1 can lead to aberrant methylation patterns contributing to oncogenesis through alteration of tumor suppressor genes. Additionally schizophrenia has an association with SETDB1 where improper histone modification can impact gene expression programs critical for neurological function. In these contexts proteins such as DNMT1 also play a role as they collaborate in epigenetic modifications linked to the disease development.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3 (H3K9me3) (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones (PubMed : 11959841, PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). H3 'Lys-9' trimethylation is coordinated with DNA methylation (PubMed : 12869583, PubMed : 14536086, PubMed : 27237050, PubMed : 39096901). Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation (PubMed : 14536086). Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1 (PubMed : 14536086). SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins (PubMed : 24623306). Required for HUSH-mediated heterochromatin formation and gene silencing (PubMed : 27732843). Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells (PubMed : 24623306). Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs) (PubMed : 24623306). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway (PubMed : 37938770). Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing (PubMed : 24623306). The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions (PubMed : 27029610). Also catalyzes mono- and dimethylation of 'Lys-9' of free histone H3 (H3K9me1 and H3K9me2) during translation in the cytoplasm, which is then transported to the nucleus and incorporated into nucleosomes (PubMed : 26405197).. Isoform 2. Lacks all domains required for histone methyltransferase activity.
See full target information SETDB1

Additional targets

Setdb1
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Product promise

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