AB272023

Anti-KMT2A / MLL antibody

Name: Anti-KMT2A / MLL antibody (ab272023) | Abcam
Brand: Abcam Limited
SKU: AB272023
Price: 530 USD
Availability: InStock

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(5 Publications)

Rabbit Polyclonal KMT2A / MLL antibody. Suitable for IP, WB, ChIP/Chip and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human KMT2A aa 2700-2800.

View Alternative Names

ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, KMT2A, Histone-lysine N-methyltransferase 2A, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Cysteine methyltransferase KMT2A, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, Trithorax-like protein, Zinc finger protein HRX

3 Images

Supplier Data

Immunoprecipitation - Anti-KMT2A / MLL antibody (AB272023)

KMT2A was immunoprecipitated from HEK293T whole cell lysate (1 mg per IP reaction, 20% loaded) with ab272023 at 3 μg per reaction. Western blot was performed on the immunoprecipitate using ab272023 at 1 μg/mL.

Lane 1 : rabbit anti-MLL1 IP in HEK293T whole cell lysate.

Lane 2 : ab272023 IP in HEK293T whole cell lysate.

Lane 3 : Control IgG in HEK293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 3 minutes

All lanes:

Immunoprecipitation - Anti-KMT2A / MLL antibody (ab272023)

Predicted band size: 432 kDa

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ChIP on chip - Anti-KMT2A / MLL antibody (AB272023)

ChIP/Chip

Supplier Data

ChIP on chip - Anti-KMT2A / MLL antibody (AB272023)

ChIP-chip scatter plot of ab272023 enriched DNA binding sites versus input reference DNA.

Plot A. 10 μg of ab272023 was used to immunoprecipitate chromatin from K562 cells according to Ren et al (Genes Dev. 2002 16 : 245-256). Immunoprecipitatesd DNA and reference DNA were amplified via ligation-mediated PCR and the products labeled with fluorescent dUTPs. The labeled ChIP and reference DNA were pooled, hybridized to a DNA microarray, and analyzed. Data points below the +3 SD curve (red line) represent significantly enriched binding sites.

Plot B. As a control, a similar experiment was performed using normal rabbit IgG. Compared to the ab272023 ChIP, normal rabbit IgG showed little enrichment.

Supplier Data

Western blot - Anti-KMT2A / MLL antibody (AB272023)

All lanes:

Western blot - Anti-KMT2A / MLL antibody (ab272023) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 432 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP, ChIP/Chip

applications

Immunogen

Synthetic Peptide within Human KMT2A aa 2700-2800. The exact immunogen used to generate this antibody is proprietary information.

Q03164

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ChIPChip" : {"fullname" : "ChIP on chip", "shortname":"ChIP/Chip"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "ChIPChip-species-checked": "testedAndGuaranteed", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "Use 10 μg." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ChIPChip-species-checked": "predicted", "ChIPChip-species-dilution-info": "", "ChIPChip-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2A protein also known as MLL (Mixed-Lineage Leukemia) is a large protein with a molecular weight of approximately 431 kDa. This protein functions as a histone methyltransferase which means it adds methyl groups to histone proteins specifically at the lysine 4 position on histone H3 (H3K4). This modification impacts chromatin structure and gene expression. KMT2A/MLL is chiefly expressed in hematopoietic stem cells and various tissue types indicating its broad role in regulation across the body. The protein features a SET domain a characteristic motif for proteins involved in chromatin modification.

Biological function summary

This methyltransferase plays a significant role in regulating gene expression required for normal hematopoietic development and maintenance. KMT2A/MLL forms part of the multi-protein complex called COMPASS-like complex which is essential for its function in methylation activity. Within this complex it associates with other proteins like WDR5 RBBP5 and ASH2L that collaborate to control transcriptional elongation. KMT2A/MLL is also involved in maintaining the expression of various homeobox (HOX) genes which are critical for embryonic development and cell differentiation.

Pathways

KMT2A/MLL plays an integral role in hematopoietic and developmental signaling pathways. One key pathway is the Wnt signaling pathway important for regulating stem cell pluripotency and cell fate decisions. Another pathway is the Notch signaling pathway important for cell differentiation processes. KMT2A interacts with proteins within these pathways such as CXXC1 which links it to the DNA binding properties required for regulating target gene expression.

KMT2A/MLL is closely linked to leukemia notably acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). A significant factor contributing to these associations is the gene fusions involving KMT2A often created during chromosomal translocations which result in oncogenic activation. In these leukemias it can form fusion proteins like MLL-AF4 impacting the normal regulatory activities of KMT2A. This aberrant interaction with proteins such as MEN1 can contribute to oncogenic processes by altering transcription and leading to uncontrolled cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Histone methyltransferase that plays an essential role in early development and hematopoiesis (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 26886794). Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac) (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 24235145, PubMed : 26886794). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 25561738, PubMed : 26886794). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity (PubMed : 19187761, PubMed : 26886794). Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9' (PubMed : 19187761). Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state (PubMed : 20010842). Required for transcriptional activation of HOXA9 (PubMed : 12453419, PubMed : 20010842, PubMed : 20677832). Promotes PPP1R15A-induced apoptosis (PubMed : 10490642). Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer (By similarity). Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin (By similarity). Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate (PubMed : 24235145).

See full target information KMT2A

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature genetics 56:1890-1902 PubMed39227744

2024

In vivo CRISPR screens identify a dual function of MEN1 in regulating tumor-microenvironment interactions.

Applications

Unspecified application

Species

Unspecified reactive species

Peiran Su,Yin Liu,Tianyi Chen,Yibo Xue,Yong Zeng,Guanghui Zhu,Sujun Chen,Mona Teng,Xinpei Ci,Mengdi Guo,Michael Y He,Jun Hao,Vivian Chu,Wenxi Xu,Shiyan Wang,Parinaz Mehdipour,Xin Xu,Sajid A Marhon,Fraser Soares,Nhu-An Pham,Bell Xi Wu,Peter Hyunwuk Her,Shengrui Feng,Najd Alshamlan,Maryam Khalil,Rehna Krishnan,Fangyou Yu,Chang Chen,Francis Burrows,Razqallah Hakem,Mathieu Lupien,Shane Harding,Benjamin H Lok,Catherine O'Brien,Alejandro Berlin,Daniel D De Carvalho,David G Brooks,Daniel Schramek,Ming-Sound Tsao,Housheng Hansen He

BMC medicine 22:57 PubMed38317232

2024

Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Wu,Chuanmei Qin,Fuju Tian,Xueqing Liu,Jianing Hu,Fan Wu,Cailian Chen,Yi Lin

Nature communications 14:5685 PubMed37709746

2023

KMT2A associates with PHF5A-PHF14-HMG20A-RAI1 subcomplex in pancreatic cancer stem cells and epigenetically regulates their characteristics.

Applications

Unspecified application

Species

Unspecified reactive species

Mai Abdel Mouti,Siwei Deng,Martin Pook,Jessica Malzahn,Aniko Rendek,Stefania Militi,Reshma Nibhani,Zahir Soonawalla,Udo Oppermann,Chang-Il Hwang,Siim Pauklin

Molecular medicine (Cambridge, Mass.) 28:76 PubMed35768768

2022

Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Yin,Yaofa Lin,Peilin Wang,Jun Zhou,Haodong Lin

Frontiers in neuroanatomy 14:36 PubMed32792914

2020

Mixed Lineage Leukemia 1 Promoted Neuron Apoptosis in Ischemic Penumbra Regulating ASK-1/TNF-α Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Feng,Liu Jie,Lv Guimin,Wang Xi

View all publications

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Product promise

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Anti-KMT2A / MLL antibody

Immunoprecipitation - Anti-KMT2A / MLL antibody (AB272023)

ChIP on chip - Anti-KMT2A / MLL antibody (AB272023)

Western blot - Anti-KMT2A / MLL antibody (AB272023)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Complementary Products

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Secondary Antibodies

Primary Antibodies

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