AB32400

Anti-KMT2A / MLL antibody [mmN4.4]

Name: Anti-KMT2A / MLL antibody [mmN4.4] (ab32400) | Abcam
Brand: Abcam Limited
SKU: AB32400
Price: 480 USD
Availability: InStock
Rating: 1 (1 reviews)

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(1 Review)

(11 Publications)

Mouse Monoclonal KMT2A / MLL antibody. Suitable for IHC-P, Flow Cyt (Intra), WB and reacts with Human, Transfected cell lysate samples. Cited in 11 publications.

View Alternative Names

ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, KMT2A, Histone-lysine N-methyltransferase 2A, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Cysteine methyltransferase KMT2A, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, Trithorax-like protein, Zinc finger protein HRX

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

IHC image of ab32400 staining in human lung adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab32400, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry (Intracellular) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

Overlay histogram showing HeLa cells stained with ab32400 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32400, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

All lanes:

Western blot - Anti-KMT2A / MLL antibody [mmN4.4] (ab32400) at 10 µg/mL

All lanes:

Lysate from transfected cells overexpressing KMT2A/MLL

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Predicted band size: 432 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mmN4.4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1 µg for 10^6 Cells", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "10 µg/mL", "WB-species-notes": "" } } }

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Product details

We can conjugate this antibody to FITC for you (please see ab150234 for details).

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2A protein also known as MLL (Mixed-Lineage Leukemia) is a large protein with a molecular weight of approximately 431 kDa. This protein functions as a histone methyltransferase which means it adds methyl groups to histone proteins specifically at the lysine 4 position on histone H3 (H3K4). This modification impacts chromatin structure and gene expression. KMT2A/MLL is chiefly expressed in hematopoietic stem cells and various tissue types indicating its broad role in regulation across the body. The protein features a SET domain a characteristic motif for proteins involved in chromatin modification.

Biological function summary

This methyltransferase plays a significant role in regulating gene expression required for normal hematopoietic development and maintenance. KMT2A/MLL forms part of the multi-protein complex called COMPASS-like complex which is essential for its function in methylation activity. Within this complex it associates with other proteins like WDR5 RBBP5 and ASH2L that collaborate to control transcriptional elongation. KMT2A/MLL is also involved in maintaining the expression of various homeobox (HOX) genes which are critical for embryonic development and cell differentiation.

Pathways

KMT2A/MLL plays an integral role in hematopoietic and developmental signaling pathways. One key pathway is the Wnt signaling pathway important for regulating stem cell pluripotency and cell fate decisions. Another pathway is the Notch signaling pathway important for cell differentiation processes. KMT2A interacts with proteins within these pathways such as CXXC1 which links it to the DNA binding properties required for regulating target gene expression.

KMT2A/MLL is closely linked to leukemia notably acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). A significant factor contributing to these associations is the gene fusions involving KMT2A often created during chromosomal translocations which result in oncogenic activation. In these leukemias it can form fusion proteins like MLL-AF4 impacting the normal regulatory activities of KMT2A. This aberrant interaction with proteins such as MEN1 can contribute to oncogenic processes by altering transcription and leading to uncontrolled cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that plays an essential role in early development and hematopoiesis (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 26886794). Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac) (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 24235145, PubMed : 26886794). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 12453419, PubMed : 15960975, PubMed : 19187761, PubMed : 19556245, PubMed : 20677832, PubMed : 21220120, PubMed : 25561738, PubMed : 26886794). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity (PubMed : 19187761, PubMed : 26886794). Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9' (PubMed : 19187761). Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state (PubMed : 20010842). Required for transcriptional activation of HOXA9 (PubMed : 12453419, PubMed : 20010842, PubMed : 20677832). Promotes PPP1R15A-induced apoptosis (PubMed : 10490642). Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer (By similarity). Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin (By similarity). Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate (PubMed : 24235145).

See full target information KMT2A

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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:110276 PubMed39109172

2024

Loss of ZNF408 attenuates STING-mediated immune surveillance in breast carcinogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Cheng,Chunyu Yu,Yan Zhang,Yani Peng,Yuncheng Liu,Hangwei Fa,Lu Xia,Leyi Qin,Sudun Guan,Xiaodi Wu,Jiajing Wu,Yue Wang,Jianying Liu,Luyang Sun,Jing Liang,Yongfeng Shang

BMC medicine 22:57 PubMed38317232

2024

Epigenetic drug screening for trophoblast syncytialization reveals a novel role for MLL1 in regulating fetoplacental growth.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Wu,Chuanmei Qin,Fuju Tian,Xueqing Liu,Jianing Hu,Fan Wu,Cailian Chen,Yi Lin

Scientific reports 11:1537 PubMed33452395

2021

EBF1 drives hallmark B cell gene expression by enabling the interaction of PAX5 with the MLL H3K4 methyltransferase complex.

Applications

Unspecified application

Species

Unspecified reactive species

Charles E Bullerwell,Philippe Pierre Robichaud,Pierre M L Deprez,Andrew P Joy,Gabriel Wajnberg,Darwin D'Souza,Simi Chacko,Sébastien Fournier,Nicolas Crapoulet,David A Barnett,Stephen M Lewis,Rodney J Ouellette

Frontiers in neuroanatomy 14:36 PubMed32792914

2020

Mixed Lineage Leukemia 1 Promoted Neuron Apoptosis in Ischemic Penumbra Regulating ASK-1/TNF-α Complex.

Applications

Unspecified application

Species

Unspecified reactive species

Zhang Feng,Liu Jie,Lv Guimin,Wang Xi

Experimental and therapeutic medicine 19:3267-3274 PubMed32266022

2020

lncRNA MIAT promotes cell invasion and migration in esophageal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Weiguo Zhang,Qiang Chen,Caipeng Lei

Cell 175:1887-1901.e18 PubMed30550787

2018

Asymmetric Expression of LincGET Biases Cell Fate in Two-Cell Mouse Embryos.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaqiang Wang,Leyun Wang,Guihai Feng,Yukai Wang,Yufei Li,Xin Li,Chao Liu,Guanyi Jiao,Cheng Huang,Junchao Shi,Tong Zhou,Qi Chen,Zhonghua Liu,Wei Li,Qi Zhou

International journal of clinical and experimental pathology 7:760-7 PubMed24551300

2014

Expression of p300 and CBP is associated with poor prognosis in small cell lung cancer.

Applications

IHC-P

Species

Human

Yina Gao,Jingshu Geng,Xuan Hong,Jiping Qi,Yang Teng,Yang Yang,Di Qu,Gongyan Chen

Proceedings of the National Academy of Sciences of 110:11511-6 PubMed23798388

2013

miR-9 is an essential oncogenic microRNA specifically overexpressed in mixed lineage leukemia-rearranged leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Chen,Colles Price,Zejuan Li,Yuanyuan Li,Donglin Cao,Anissa Wiley,Chunjiang He,Sandeep Gurbuxani,Rejani B Kunjamma,Hao Huang,Xi Jiang,Stephen Arnovitz,Mengyi Xu,Gia-Ming Hong,Abdel G Elkahloun,Mary Beth Neilly,Mark Wunderlich,Richard A Larson,Michelle M Le Beau,James C Mulloy,Paul P Liu,Janet D Rowley,Jianjun Chen

Cancer cell 14:36-46 PubMed18598942

2008

Menin critically links MLL proteins with LEDGF on cancer-associated target genes.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiko Yokoyama,Michael L Cleary

Cell 123:207-18 PubMed16239140

2005

The menin tumor suppressor protein is an essential oncogenic cofactor for MLL-associated leukemogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Akihiko Yokoyama,Tim C P Somervaille,Kevin S Smith,Orit Rozenblatt-Rosen,Matthew Meyerson,Michael L Cleary

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT2A / MLL antibody [mmN4.4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

Flow Cytometry (Intracellular) - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

Western blot - Anti-KMT2A / MLL antibody [mmN4.4] (AB32400)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

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