Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal

• WB

Supplier Data

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (AB315464)

Lysates were freshly made and used immediately to minimize protein degradation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody has cross reaction with human KMT2B, band at 80 kDa represents KMT2B C-terminal protein.

All lanes:

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 4:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa,80 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (AB315464)

In Western blot, ab315464 was shown to bind specifically to KMT2A. A band was observed at 180 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in KMT2A knockout cell line.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody has cross reaction with human KMT2B, band at 80 kDa represents KMT2B C-terminal protein.

Lysates were freshly made and used immediately to minimize protein degradation.

There is an unknown band at ~20 kDa.

In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (ab176842) staining at 1/100000 dilution.

All lanes:

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg

Lane 2:

KMT2A knockout HAP1 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 180 kDa,80 kDa,15 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (AB315464)

This antibody has cross reaction with human KMT2B.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All the recombinant proteins were made in-house.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464) at 1/1000 dilution

Lane 1:

His-tagged human KMT2A recommbinant protein fragment at 10 ng

Lane 2:

His-tagged human KMT2B recommbinant protein fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 20 kDa,37 kDa

false

Exposure time: 3s