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Knockout Tested Rabbit Recombinant Monoclonal KMT2A / MLL antibody. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

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Images

Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (AB315464), expandable thumbnail
  • Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (AB315464), expandable thumbnail
  • Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (AB315464), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)IP
Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Mouse
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Tested
Not recommended
Not recommended
Not recommended
Not recommended
Recombinant fragment - Human
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

-

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Species
Recombinant fragment - Human
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Recombinant fragment - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Histone methyltransferase that plays an essential role in early development and hematopoiesis (PubMed:12453419, PubMed:15960975, PubMed:19187761, PubMed:19556245, PubMed:20677832, PubMed:21220120, PubMed:26886794). Catalytic subunit of the MLL1/MLL complex, a multiprotein complex that mediates both methylation of 'Lys-4' of histone H3 (H3K4me) complex and acetylation of 'Lys-16' of histone H4 (H4K16ac) (PubMed:12453419, PubMed:15960975, PubMed:19187761, PubMed:19556245, PubMed:20677832, PubMed:21220120, PubMed:24235145, PubMed:26886794). Catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:12453419, PubMed:15960975, PubMed:19187761, PubMed:19556245, PubMed:20677832, PubMed:21220120, PubMed:25561738, PubMed:26886794). Has weak methyltransferase activity by itself, and requires other component of the MLL1/MLL complex to obtain full methyltransferase activity (PubMed:19187761, PubMed:26886794). Has no activity toward histone H3 phosphorylated on 'Thr-3', less activity toward H3 dimethylated on 'Arg-8' or 'Lys-9', while it has higher activity toward H3 acetylated on 'Lys-9' (PubMed:19187761). Binds to unmethylated CpG elements in the promoter of target genes and helps maintain them in the nonmethylated state (PubMed:20010842). Required for transcriptional activation of HOXA9 (PubMed:12453419, PubMed:20010842, PubMed:20677832). Promotes PPP1R15A-induced apoptosis (PubMed:10490642). Plays a critical role in the control of circadian gene expression and is essential for the transcriptional activation mediated by the CLOCK-BMAL1 heterodimer (By similarity). Establishes a permissive chromatin state for circadian transcription by mediating a rhythmic methylation of 'Lys-4' of histone H3 (H3K4me) and this histone modification directs the circadian acetylation at H3K9 and H3K14 allowing the recruitment of CLOCK-BMAL1 to chromatin (By similarity). Also has auto-methylation activity on Cys-3882 in absence of histone H3 substrate (PubMed:24235145).

Additional Targets

KMT2B

Alternative names

ALL1, CXXC7, HRX, HTRX, MLL, MLL1, TRX1, KMT2A, Histone-lysine N-methyltransferase 2A, Lysine N-methyltransferase 2A, ALL-1, CXXC-type zinc finger protein 7, Cysteine methyltransferase KMT2A, Myeloid/lymphoid or mixed-lineage leukemia, Myeloid/lymphoid or mixed-lineage leukemia protein 1, Trithorax-like protein, Zinc finger protein HRX

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal KMT2A / MLL antibody. Suitable for WB and reacts with Human, Mouse, Rat, Recombinant fragment - Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR28282-16
Purification technique
Affinity purification Protein A
Specificity

This antibody has cross reaction with human KMT2B.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

KMT2A also known as MLL and KMT2B also called MLL4 are essential histone methyltransferases. These proteins have a molecular mass of approximately 430-431 kDa. KMT2A is expressed mainly in the cell nucleus and functions as a transcriptional co-regulator. MLL4 is similar and also found in various tissues with considerable expression in the developing brain. Both KMT2A and MLL4 perform important roles in the modulation of chromatin structure through methylation of histone H3 at lysine 4 influencing gene expression.

Biological function summary

KMT2A and MLL4 participate in transcriptional regulation. They are components of large protein complexes that include WDR5 RBBP5 and ASH2L which facilitate chromatin remodeling and gene activation. By modifying chromatin states these proteins influence the expression of genes involved in development and differentiation. They are particularly active during early embryonic development playing significant roles in hematopoiesis and neuronal lineage specification ensuring proper gene activation patterns necessary for these processes.

Pathways

These proteins significantly contribute to the regulation of gene expression pathways such as the Wnt signaling and the HOX gene regulatory network. Their activity within the Wnt signaling pathway helps control cell fate decisions and cellular proliferation. KMT2A and MLL4 interact with other pathway-related proteins like β-catenin facilitating the transcription of target genes. In the context of the HOX gene regulatory network these proteins ensure proper developmental patterns by interacting with other transcription factors to establish body axis formation.

Associated diseases and disorders

Malfunctioning of KMT2A and MLL4 links to various conditions like acute leukemia and Kabuki syndrome. In leukemia chromosomal translocations involving KMT2A lead to fusion proteins that disrupt normal gene regulation contributing to the malignancy. These fusion proteins often partner with other proteins such as AF4 ENL or ELL to sustain the leukemogenic process. Similarly mutations in MLL4 associate with Kabuki syndrome a disorder characterized by intellectual disability and growth delay involving its connection with proteins like KDM6A which regulate chromatin structure and function.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464), expandable thumbnail

    Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464)

    In Western blot, ab315464 was shown to bind specifically to KMT2A. A band was observed at 180 kDa in wild-type HAP1 cell lysates whereas no signal observed at this size in KMT2A knockout cell line.

    This antibody has cross reaction with human KMT2B, band at 80 kDa represents KMT2B C-terminal protein.

    Lysates were freshly made and used immediately to minimize protein degradation.

    There is an unknown band at ~20 kDa.

    In Western blot, Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade (Anti-Histone H3 antibody [EPR16987] - Nuclear Marker and ChIP Grade ab176842) staining at 1/100000 dilution.

    All lanes: Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464) at 1/1000 dilution

    Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: KMT2A knockout HAP1 whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Performed under reducing conditions.

    Observed band size: 180 kDa, 80 kDa, 15 kDa

    Exposure time: 81s

  • Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464), expandable thumbnail

    Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464)

    Lysates were freshly made and used immediately to minimize protein degradation.

    This antibody has cross reaction with human KMT2B, band at 80 kDa represents KMT2B C-terminal protein.

    All lanes: Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464) at 1/1000 dilution

    Lane 1: HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 180 kDa, 80 kDa

    Exposure time: 180s

  • Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464), expandable thumbnail

    Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464)

    This antibody has cross reaction with human KMT2B.

    All the recombinant proteins were made in-house.

    In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

    All lanes: Western blot - Anti-KMT2A / MLL + KMT2B / MLL4 antibody [EPR28282-16] - C-terminal (ab315464) at 1/1000 dilution

    Lane 1: His-tagged human KMT2A recommbinant protein fragment at 10 ng with 5% NFDM/TBST

    Lane 2: His-tagged human KMT2B recommbinant protein fragment at 10 ng with 5% NFDM/TBST

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 20 kDa, 37 kDa

    Exposure time: 3s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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