AB56770

Anti-KMT2B / MLL4 antibody [4C10]

Name: Anti-KMT2B / MLL4 antibody [4C10] (ab56770) | Abcam
Brand: Abcam Limited
SKU: AB56770
Price: 585 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Mouse Monoclonal KMT2B / MLL4 antibody. Suitable for Flow Cyt, WB and reacts with Human, Recombinant fragment - Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KMT2B aa 800-950.

View Alternative Names

HRX2, KIAA0304, MLL2, MLL4, TRX2, WBP7, KMT2B, Histone-lysine N-methyltransferase 2B, Lysine N-methyltransferase 2B, Myeloid/lymphoid or mixed-lineage leukemia protein 4, Trithorax homolog 2, WW domain-binding protein 7, WBP-7

2 Images

Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (AB56770)

Flow Cyt

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Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (AB56770)

Overlay histogram showing HL60 cells stained with ab56770 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab56770, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

This image was generated using the ascites version of the product.

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Western blot - Anti-KMT2B / MLL4 antibody [4C10] (AB56770)

Western blot against tagged recombinant protein immunogen using ab56770 MLL4 antibody at 1ug/ml. Predicted band size of immunogen is 36 kDa

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-KMT2B / MLL4 antibody [4C10] (ab56770)

Predicted band size: 294 kDa

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Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

4C10

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

Flow Cyt, WB

applications

Immunogen

Recombinant Fragment Protein within Human KMT2B aa 800-950. The exact immunogen used to generate this antibody is proprietary information.

Q9UMN6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.</p>" } } }

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Product details

This product was changed from ascites to tissue culture supernatant on 11^th June 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.4

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT2B also known as MLL4 is a protein complex that functions as a histone methyltransferase. It specifically catalyzes the methylation of histone H3 on lysine 4 (H3K4) a modification associated with transcriptional activation. KMT2B has a molecular mass of approximately 296 kDa and is widely expressed in human tissues with notable expression in the brain heart and skeletal muscle. This protein is part of the COMPASS-like complexes which play significant roles in chromatin remodeling.

Biological function summary

KMT2B contributes to regulating gene expression. As part of the larger COMPASS-like complex it cooperates with other regulatory proteins to modify chromatin structure facilitating easier access of transcriptional machinery to DNA. This action influences the activation of genes important for cell differentiation and development. KMT2B interacts with several cofactors including WRAD components (WDR5 RBBP5 ASH2L and DPY30) enhancing its enzymatic activity.

Pathways

KMT2B functions prominently in signaling mechanisms like the Wnt and MAPK pathways. These pathways are integral to cell growth and differentiation. The protein intricately connects with other pathways’ proteins including beta-catenin in the Wnt pathway and ERK1/2 in the MAPK pathway. These interactions highlight KMT2B's role in regulating cellular functions and development.

KMT2B has associations with neurodevelopmental disorders and specific forms of cancer. Mutations in KMT2B can lead to dystonia a movement disorder characterized by involuntary muscle contractions. In cancer alterations affect pathways involving oncogenic factors such as MYC highlighting the critical connections between KMT2B and cellular growth regulation. Understanding these links provides insights into potential therapeutic targets for related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 17707229, PubMed : 25561738). Likely plays a redundant role with KMT2C in enriching H3K4me1 marks on primed and active enhancer elements (PubMed : 24081332). Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed : 17707229). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).

See full target information KMT2B

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Cell biology and toxicology 41:52 PubMed40000487

2025

Molecular mechanism of ZC3H13 -mediated ferroptosis in doxorubicin resistance of triple negative breast cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Li Huang,Lei Han,Shuai Liang,Guohui Han

Molecular carcinogenesis 63:371-383 PubMed37975495

2023

LncRNA SEMA3B-AS1 suppresses the tumor-initiating characteristics of triple negative breast cancer via engaging in MLL4-mediated H3K4 trimethylation.

Applications

Unspecified application

Species

Unspecified reactive species

Debo Chen,Zhishan Chen,Zhitang Wang,Chengye Hong,Qinglan Wang,Peidong Yang,Zhidong Huang,Weibin Lian,Yiqin Huang,Wei Fu,Junjing Li,Zhipeng Hong

Cell death discovery 8:350 PubMed35927251

2022

M2-like tumor-associated macrophages transmit exosomal miR-27b-3p and maintain glioblastoma stem-like cell properties.

Applications

Unspecified application

Species

Unspecified reactive species

Guifang Zhao,Lijuan Ding,Hongquan Yu,Weiyao Wang,Huan Wang,Yao Hu,Lingsha Qin,Guangce Deng,Buqing Xie,Guofeng Li,Ling Qi

Journal of oncology 2022:5054324 PubMed35578598

2022

AHCYL1 Is a Novel Biomarker for Predicting Prognosis and Immunotherapy Response in Colorectal Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xubin Li,Mengqiao Zhang,Xue Yu,Mei Xue,Xiaowei Li,Chao Ma,Wei Jia,Qiang Gao,Chunbo Kang

Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association 22:705-722 PubMed30656427

2019

MiR-6872 host gene SEMA3B and its antisense lncRNA SEMA3B-AS1 function synergistically to suppress gastric cardia adenocarcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Guo,Xiaoliang Liang,Lei Liu,Yanli Guo,Supeng Shen,Jia Liang,Zhiming Dong

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT2B / MLL4 antibody [4C10]

Flow Cytometry - Anti-KMT2B / MLL4 antibody [4C10] (AB56770)

Western blot - Anti-KMT2B / MLL4 antibody [4C10] (AB56770)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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