Goat Polyclonal KMT2B / MLL4 antibody. Suitable for IHC-P, ELISA and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KMT2B aa 2700 to C-terminus.
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Publications
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- Frontiers in oncology 12:8551672022Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJyothishmathi Swaminathan,Shinji Maegawa,Shavali Shaik,Ajay Sharma,Javiera Bravo-Alegria,Lei Guo,Lin Xu,Arif Harmanci,Vidya GopalakrishnanPubMed 35600406
- PloS one 11:e01660902016Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification.
- Nature communications 7:116882016Rebalancing gene haploinsufficiency in vivo by targeting chromatin.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilomena Gabriella Fulcoli et. al.PubMed 27256596
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human KMT2B aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UMN6
Reactivity data
Select an application| IHC-P | ELISA | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Predicted | Predicted |
| Rat | Predicted | Predicted |
| Common marmoset | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Gorilla | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Zebrafish | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset | Dilution info - | Notes - |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset | Dilution info - | Notes - |
Associated Products
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Target data
Function
Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:17707229, PubMed:25561738). Likely plays a redundant role with KMT2C in enriching H3K4me1 marks on primed and active enhancer elements (PubMed:24081332). Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed:17707229). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).
Alternative names
HRX2, KIAA0304, MLL2, MLL4, TRX2, WBP7, KMT2B, Histone-lysine N-methyltransferase 2B, Lysine N-methyltransferase 2B, Myeloid/lymphoid or mixed-lineage leukemia protein 4, Trithorax homolog 2, WW domain-binding protein 7, WBP-7
Recommended products
Goat Polyclonal KMT2B / MLL4 antibody. Suitable for IHC-P, ELISA and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KMT2B aa 2700 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human KMT2B aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UMN6
- Purification technique
- Affinity purification Protein G
- Concentration
- Purification notes
Immunoaffinity purified
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
KMT2B also known as MLL4 is a protein complex that functions as a histone methyltransferase. It specifically catalyzes the methylation of histone H3 on lysine 4 (H3K4) a modification associated with transcriptional activation. KMT2B has a molecular mass of approximately 296 kDa and is widely expressed in human tissues with notable expression in the brain heart and skeletal muscle. This protein is part of the COMPASS-like complexes which play significant roles in chromatin remodeling.
- Biological function summary
KMT2B contributes to regulating gene expression. As part of the larger COMPASS-like complex it cooperates with other regulatory proteins to modify chromatin structure facilitating easier access of transcriptional machinery to DNA. This action influences the activation of genes important for cell differentiation and development. KMT2B interacts with several cofactors including WRAD components (WDR5 RBBP5 ASH2L and DPY30) enhancing its enzymatic activity.
- Pathways
KMT2B functions prominently in signaling mechanisms like the Wnt and MAPK pathways. These pathways are integral to cell growth and differentiation. The protein intricately connects with other pathways’ proteins including beta-catenin in the Wnt pathway and ERK1/2 in the MAPK pathway. These interactions highlight KMT2B's role in regulating cellular functions and development.
- Associated diseases and disorders
KMT2B has associations with neurodevelopmental disorders and specific forms of cancer. Mutations in KMT2B can lead to dystonia a movement disorder characterized by involuntary muscle contractions. In cancer alterations affect pathways involving oncogenic factors such as MYC highlighting the critical connections between KMT2B and cellular growth regulation. Understanding these links provides insights into potential therapeutic targets for related conditions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (ab104444)
ab104444, at 5 μg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (ab104444)
ab104444, at 5 μg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.
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