AB104444

Anti-KMT2B / MLL4 antibody

Name: Anti-KMT2B / MLL4 antibody (ab104444) | Abcam
Brand: Abcam Limited
SKU: AB104444
Price: 630 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(4 Publications)

Goat Polyclonal KMT2B / MLL4 antibody. Suitable for IHC-P, ELISA and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human KMT2B aa 2700 to C-terminus.

View Alternative Names

HRX2, KIAA0304, MLL2, MLL4, TRX2, WBP7, KMT2B, Histone-lysine N-methyltransferase 2B, Lysine N-methyltransferase 2B, Myeloid/lymphoid or mixed-lineage leukemia protein 4, Trithorax homolog 2, WW domain-binding protein 7, WBP-7

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (AB104444)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (AB104444)

ab104444, at 5 μg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (AB104444)

ab104444, at 5 μg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ELISA, IHC-P

applications

Immunogen

Synthetic Peptide within Human KMT2B aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UMN6

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Common marmoset": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Gorilla": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Zebrafish": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Immunoaffinity purified

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

KMT2B also known as MLL4 is a protein complex that functions as a histone methyltransferase. It specifically catalyzes the methylation of histone H3 on lysine 4 (H3K4) a modification associated with transcriptional activation. KMT2B has a molecular mass of approximately 296 kDa and is widely expressed in human tissues with notable expression in the brain heart and skeletal muscle. This protein is part of the COMPASS-like complexes which play significant roles in chromatin remodeling.

Biological function summary

KMT2B contributes to regulating gene expression. As part of the larger COMPASS-like complex it cooperates with other regulatory proteins to modify chromatin structure facilitating easier access of transcriptional machinery to DNA. This action influences the activation of genes important for cell differentiation and development. KMT2B interacts with several cofactors including WRAD components (WDR5 RBBP5 ASH2L and DPY30) enhancing its enzymatic activity.

Pathways

KMT2B functions prominently in signaling mechanisms like the Wnt and MAPK pathways. These pathways are integral to cell growth and differentiation. The protein intricately connects with other pathways’ proteins including beta-catenin in the Wnt pathway and ERK1/2 in the MAPK pathway. These interactions highlight KMT2B's role in regulating cellular functions and development.

KMT2B has associations with neurodevelopmental disorders and specific forms of cancer. Mutations in KMT2B can lead to dystonia a movement disorder characterized by involuntary muscle contractions. In cancer alterations affect pathways involving oncogenic factors such as MYC highlighting the critical connections between KMT2B and cellular growth regulation. Understanding these links provides insights into potential therapeutic targets for related conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery predominantly forms H3K4me1 and H3K4me2 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 17707229, PubMed : 25561738). Likely plays a redundant role with KMT2C in enriching H3K4me1 marks on primed and active enhancer elements (PubMed : 24081332). Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed : 17707229). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).

See full target information KMT2B

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine (Cambridge, Mass.) 28:76 PubMed35768768

2022

Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Yin,Yaofa Lin,Peilin Wang,Jun Zhou,Haodong Lin

Frontiers in oncology 12:855167 PubMed35600406

2022

Cross-Talk Between Histone Methyltransferases and Demethylases Regulate REST Transcription During Neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jyothishmathi Swaminathan,Shinji Maegawa,Shavali Shaik,Ajay Sharma,Javiera Bravo-Alegria,Lei Guo,Lin Xu,Arif Harmanci,Vidya Gopalakrishnan

PloS one 11:e0166090 PubMed27806114

2016

Regulation of IL-20 Expression by Estradiol through KMT2B-Mediated Epigenetic Modification.

Applications

IHC-P, ChIP

Species

Human, Human

Chia-Hsin Su,I-Hsuan Lin,Tsai-Yu Tzeng,Wen-Ting Hsieh,Ming-Ta Hsu

Nature communications 7:11688 PubMed27256596

2016

Rebalancing gene haploinsufficiency in vivo by targeting chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Filomena Gabriella Fulcoli,Monica Franzese,Xiangyang Liu,Zhen Zhang,Claudia Angelini,Antonio Baldini

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-KMT2B / MLL4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (AB104444)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (AB104444)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise