Skip to main content

Goat Polyclonal KMT2B / MLL4 antibody. Suitable for IHC-P, ELISA and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KMT2B aa 2700 to C-terminus.


Images

Key facts

Isotype

IgG

Host species

Goat

Storage buffer

pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Synthetic Peptide within Human KMT2B aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UMN6
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PELISA
Human
Tested
Expected
Mouse
Predicted
Predicted
Rat
Predicted
Predicted
Common marmoset
Predicted
Predicted
Cow
Predicted
Predicted
Gorilla
Predicted
Predicted
Monkey
Predicted
Predicted
Pig
Predicted
Predicted
Zebrafish
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

5 µg/mL

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Predicted
Predicted

Species

Mouse, Rat, Cow, Pig, Monkey, Zebrafish, Gorilla, Common marmoset

Dilution info

-

Notes

-

Target data

Function

Histone methyltransferase that methylates 'Lys-4' of histone H3 (PubMed:17707229). H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation (PubMed:17707229). Plays a central role in beta-globin locus transcription regulation by being recruited by NFE2 (PubMed:17707229). Plays an important role in controlling bulk H3K4me during oocyte growth and preimplantation development (By similarity). Required during the transcriptionally active period of oocyte growth for the establishment and/or maintenance of bulk H3K4 trimethylation (H3K4me3), global transcriptional silencing that preceeds resumption of meiosis, oocyte survival and normal zygotic genome activation (By similarity).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Goat Polyclonal KMT2B / MLL4 antibody. Suitable for IHC-P, ELISA and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human KMT2B aa 2700 to C-terminus.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen
  • Synthetic Peptide within Human KMT2B aa 2700 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UMN6
Purification technique

Affinity purification Protein G

Concentration
Loading...
Purification notes

Immunoaffinity purified

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological function summary

KMT2B contributes to regulating gene expression. As part of the larger COMPASS-like complex it cooperates with other regulatory proteins to modify chromatin structure facilitating easier access of transcriptional machinery to DNA. This action influences the activation of genes important for cell differentiation and development. KMT2B interacts with several cofactors including WRAD components (WDR5 RBBP5 ASH2L and DPY30) enhancing its enzymatic activity.

Activity summary

KMT2B also known as MLL4 is a protein complex that functions as a histone methyltransferase. It specifically catalyzes the methylation of histone H3 on lysine 4 (H3K4) a modification associated with transcriptional activation. KMT2B has a molecular mass of approximately 296 kDa and is widely expressed in human tissues with notable expression in the brain heart and skeletal muscle. This protein is part of the COMPASS-like complexes which play significant roles in chromatin remodeling.

Pathways

KMT2B functions prominently in signaling mechanisms like the Wnt and MAPK pathways. These pathways are integral to cell growth and differentiation. The protein intricately connects with other pathways’ proteins including beta-catenin in the Wnt pathway and ERK1/2 in the MAPK pathway. These interactions highlight KMT2B's role in regulating cellular functions and development.

Associated diseases and disorders

KMT2B has associations with neurodevelopmental disorders and specific forms of cancer. Mutations in KMT2B can lead to dystonia a movement disorder characterized by involuntary muscle contractions. In cancer alterations affect pathways involving oncogenic factors such as MYC highlighting the critical connections between KMT2B and cellular growth regulation. Understanding these links provides insights into potential therapeutic targets for related conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (ab104444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (ab104444)

    ab104444, at 5 μg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human brain cortex tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (ab104444), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2B / MLL4 antibody (ab104444)

    ab104444, at 5 μg/ml, staining KMT2B / MLL4 in formalin-fixed, paraffin-embedded human testis tissue by Immunohistochemistry. A biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen was used as the secondary antibody.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com