AB224156

Anti-KMT2D / MLL2 antibody

Name: Anti-KMT2D / MLL2 antibody (ab224156) | Abcam
Brand: Abcam Limited
SKU: AB224156
Price: 525 USD
Availability: InStock

(1 Review)

(7 Publications)

Rabbit Polyclonal KMT2D / MLL2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KMT2D aa 4350-4500.

View Alternative Names

ALR, MLL2, MLL4, KMT2D, Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2

2 Images

Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody (AB224156)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody (AB224156)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for KMT2D / MLL2 (green) using ab224156 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2D / MLL2 antibody (AB224156)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2D / MLL2 antibody (AB224156)

Paraffin-embedded human cerebral cortex tissue stained for KMT2D / MLL2 using ab224156 at 1/200 dilution in immunohistochemical analysis.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human KMT2D aa 4350-4500. The exact immunogen used to generate this antibody is proprietary information.

O14686

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The KMT2D also known as MLL2 is an important histone methyltransferase with a known mass of approximately 553 kDa. This enzyme plays a mechanical role in the methylation of histone H3 on lysine 4 (H3K4) which facilitates chromatin remodeling and transcriptional regulation. KMT2D is expressed in various tissues including the liver lungs and heart indicative of its diverse regulatory roles across different biological systems.

Biological function summary

KMT2D is an important regulatory factor that influences gene expression. It is part of a larger complex that includes other interacting proteins necessary for histone modification. Through its enzymatic activity KMT2D impacts gene transcription by modifying chromatin structure making it more or less accessible for transcription factors to bind DNA. This control over gene expression has implications in development differentiation and cellular response to external stimuli.

Pathways

KMT2D integrates into the broader framework of chromatin modification and transcriptional regulation pathways. It interacts with proteins such as ASH2L and RBBP5 which are components of the COMPASS-like complex playing roles in modifying histones to regulate gene expression dynamically. KMT2D's function intersects particularly with the Hedgehog and Wnt signaling pathways both important for developmental processes and cellular proliferation.

KMT2D has strong associations with several conditions. Mutations or dysregulation of this methyltransferase can lead to developmental disorders such as Kabuki syndrome characterized by intellectual disability and growth delays. KMT2D anomalies also appear in cancer where its dysregulation affects pathways involving the enhancer of filament genes (EFG) and histone methyltransferases like NSD2 contributing to oncogenesis by altering gene expression profiles critical for cell growth.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) (PubMed : 25561738). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed : 17500065, PubMed : 25561738). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription (PubMed : 16603732).

See full target information KMT2D

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Molecular pain 21:17448069251314857 PubMed39905828

2025

Upregulation of the neuropeptide receptor calcitonin receptor-like in the spinal cord via MLL2 in a mouse model of paclitaxel-induced peripheral neuropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Salvador Sierra,Sara M Herz,Doan On,Mikhail G Dozmorov,M Imad Damaj,Javier Gonzalez-Maeso

Clinical and translational medicine 14:e1753 PubMed38967349

2024

KMT2D-mediated H3K4me1 recruits YBX1 to facilitate triple-negative breast cancer progression through epigenetic activation of c-Myc.

Applications

Unspecified application

Species

Unspecified reactive species

Bing Yao,Mengying Xing,Xiangwei Zeng,Ming Zhang,Que Zheng,Zhi Wang,Bo Peng,Shuang Qu,Lingyun Li,Yucui Jin,Haitao Li,Hongyan Yuan,Quan Zhao,Changyan Ma

Biomedicines 10: PubMed36289705

2022

FYCO1 Increase and Effect of Arimoclomol-Treatment in Human -Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Anne-Katrin Guettsches,Nancy Meyer,René P Zahedi,Teresinha Evangelista,Thomas Muentefering,Tobias Ruck,Emmanuelle Lacene,Christoph Heute,Humberto Gonczarowska-Jorge,Benedikt Schoser,Sabine Krause,Andreas Hentschel,Matthias Vorgerd,Andreas Roos

Molecular medicine (Cambridge, Mass.) 28:76 PubMed35768768

2022

Upregulated lncARAT in Schwann cells promotes axonal regeneration by recruiting and activating proregenerative macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Gang Yin,Yaofa Lin,Peilin Wang,Jun Zhou,Haodong Lin

Journal of personalized medicine 11: PubMed34834500

2021

Expression Analysis and Mutational Status of Histone Methyltransferase at Different Upper Tract Urothelial Carcinoma Locations.

Applications

Unspecified application

Species

Unspecified reactive species

Ekaterina Laukhtina,Ursula Lemberger,Andreas Bruchbacher,Dafina Ilijazi,Stephan Korn,Florian Berndl,David D'Andrea,Martin Susani,Dmitry Enikeev,Eva Compérat,Shahrokh F Shariat,Melanie R Hassler

Frontiers in oncology 11:669560 PubMed33968779

2021

Trans-Ancestry Mutation Landscape of Hepatoblastoma Genomes in Children.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Liu,Chengwen Gao,Liping Wang,Xuemin Jian,Mingdi Ma,Tong Li,XiWei Hao,Qian Zhang,Yuanbin Chen,Jing Zhao,Haitao Niu,Chengzhan Zhu,Jie Zhao,Nan Xia,Zhiqiang Li,Qian Dong

Biomedicine & pharmacotherapy = Biomedecine & phar 115:108924 PubMed31100540

2019

KMT2D inhibits the growth and metastasis of bladder Cancer cells by maintaining the tumor suppressor genes.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Sun,Tong Wu,Xiaoliang Sun,Zilian Cui,Haiyang Zhang,Qinghua Xia,Dong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-KMT2D / MLL2 antibody

Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody (AB224156)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2D / MLL2 antibody (AB224156)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

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