Rabbit Polyclonal KMT2D / MLL2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KMT2D aa 4350-4500.
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Publications
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- Journal of personalized medicine 11:2021Expression Analysis and Mutational Status of Histone Methyltransferase at Different Upper Tract Urothelial Carcinoma Locations.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEkaterina Laukhtina,Ursula Lemberger,Andreas Bruchbacher,Dafina Ilijazi,Stephan Korn,Florian Berndl,David D'Andrea,Martin Susani,Dmitry Enikeev,Eva Compérat,Shahrokh F Shariat,Melanie R HasslerPubMed 34834500
- Frontiers in oncology 11:6695602021Trans-Ancestry Mutation Landscape of Hepatoblastoma Genomes in Children.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie Liu,Chengwen Gao,Liping Wang,Xuemin Jian,Mingdi Ma,Tong Li,XiWei Hao,Qian Zhang,Yuanbin Chen,Jing Zhao,Haitao Niu,Chengzhan Zhu,Jie Zhao,Nan Xia,Zhiqiang Li,Qian DongPubMed 33968779
- Biomedicine & pharmacotherapy = Biomedecine & phar 115:1089242019KMT2D inhibits the growth and metastasis of bladder Cancer cells by maintaining the tumor suppressor genes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPeng Sun et. al.PubMed 31100540
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human KMT2D aa 4350-4500. The exact immunogen used to generate this antibody is proprietary information. Database link O14686
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Predicted | Predicted |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) (PubMed:25561738). Part of chromatin remodeling machinery predominantly forms H3K4me1 methylation marks at active chromatin sites where transcription and DNA repair take place (PubMed:17500065, PubMed:25561738). Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription (PubMed:16603732).
Alternative names
ALR, MLL2, MLL4, KMT2D, Histone-lysine N-methyltransferase 2D, Lysine N-methyltransferase 2D, ALL1-related protein, Myeloid/lymphoid or mixed-lineage leukemia protein 2
Recommended products
Rabbit Polyclonal KMT2D / MLL2 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human KMT2D aa 4350-4500.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human KMT2D aa 4350-4500. The exact immunogen used to generate this antibody is proprietary information. Database link O14686
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The KMT2D also known as MLL2 is an important histone methyltransferase with a known mass of approximately 553 kDa. This enzyme plays a mechanical role in the methylation of histone H3 on lysine 4 (H3K4) which facilitates chromatin remodeling and transcriptional regulation. KMT2D is expressed in various tissues including the liver lungs and heart indicative of its diverse regulatory roles across different biological systems.
- Biological function summary
KMT2D is an important regulatory factor that influences gene expression. It is part of a larger complex that includes other interacting proteins necessary for histone modification. Through its enzymatic activity KMT2D impacts gene transcription by modifying chromatin structure making it more or less accessible for transcription factors to bind DNA. This control over gene expression has implications in development differentiation and cellular response to external stimuli.
- Pathways
KMT2D integrates into the broader framework of chromatin modification and transcriptional regulation pathways. It interacts with proteins such as ASH2L and RBBP5 which are components of the COMPASS-like complex playing roles in modifying histones to regulate gene expression dynamically. KMT2D's function intersects particularly with the Hedgehog and Wnt signaling pathways both important for developmental processes and cellular proliferation.
- Associated diseases and disorders
KMT2D has strong associations with several conditions. Mutations or dysregulation of this methyltransferase can lead to developmental disorders such as Kabuki syndrome characterized by intellectual disability and growth delays. KMT2D anomalies also appear in cancer where its dysregulation affects pathways involving the enhancer of filament genes (EFG) and histone methyltransferases like NSD2 contributing to oncogenesis by altering gene expression profiles critical for cell growth.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-KMT2D / MLL2 antibody (ab224156)
Paraffin-embedded human cerebral cortex tissue stained for KMT2D / MLL2 using ab224156 at 1/200 dilution in immunohistochemical analysis.
Immunocytochemistry/ Immunofluorescence - Anti-KMT2D / MLL2 antibody (ab224156)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for KMT2D / MLL2 (green) using ab224156 at 4 μg/ml in ICC/IF.
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